Journal: Blood Vessels, Thrombosis & Hemostasis

Article Title: Platelet recruitment kinetics are impacted by von Willebrand factor quality in hemostatic adjuncts

doi: 10.1016/j.bvth.2025.100076

Figure Lengend Snippet: Coagulopathic resuscitation model establishment in vitro. (A) Schematics of coagulopathy induction and resuscitation, and subsequent workflow. WB was diluted 3:7 with normal saline to induce dilutional coagulopathy (dWB) or was dosed with 75 ng/mL tPA to induce hyperfibrinolytic coagulopathy (lyWB). Cryo or FibCon was mixed 1:5 with dWB and lyWB, providing resuscitation and yielding r:dWB and r:lyWB, respectively. (B) Microfluidic assay setup schematic. Sample syringe containing resuscitated coagulopathies (rCryo:dWB, rCryo:lyWB, rFibCon:dWB, rFibCon:lyWB) was perfused via syringe pump for constant flow rate perfusion through the microfluidic flow chamber, and chambers were imaged in real time using an inverted fluorescent microscope. Images in panels A and B were created by K.A.T. and S.M.S. using BioRender.com . (C) Computational fluid dynamics simulation yielding flow streamlines through the microfluidic chamber (top). Representative phase images (bottom left) from a WB control at 500 s −1 that resulted in a BT of 4.7 minutes (bottom right). (D) BTs of dWB and lyWB upon perfusion through the microfluidic model of bleeding; assay cutoff of 1200 seconds determined by a quadrupling of the mean of WB controls. Data are reported as mean (standard deviation). N = 2 to 3. PRP, platelet-rich plasma; PPP, platelet-poor plasma; Q, flow; TF, tissue factor; tPA, tissue plasminogen activator.

Article Snippet: Hyperfibrinolytic coagulopathy was not well resuscitated with adjuncts under flow, especially at higher shear rates, in contrast to static assays, in which Cryo rescued lysis better than FibCon (LI60, ROTEM EXTEM ( ; ), consistent with previous reports.

Techniques: In Vitro, Saline, Microscopy, Control, Standard Deviation, Clinical Proteomics

Journal: Blood Vessels, Thrombosis & Hemostasis

Article Title: Platelet recruitment kinetics are impacted by von Willebrand factor quality in hemostatic adjuncts

doi: 10.1016/j.bvth.2025.100076

Figure Lengend Snippet: Cryo better resuscitates in vitro–induced dilutional coagulopathy than FibCon. (A) Representative phase images of clot formation in the injury site over the course of perfusion experiments in the microfluidic model of bleeding. (B) BTs, (C) closure frequency (the percentage of experiments that achieved complete closure), and (D) terminal clot surface area for rCryo:dWB and rFibCon:dWB at all 3 shear rates. In panel B, dotted line indicates assay cutoff at 1200 seconds and is representative of no occlusion. (E) Lag-phase duration, (F) growth rates, (G) clot lysis duration, and (H) number of total independently identified events extracted from kinetic analyses of clot formation in rCryo:dWB and rFibCon:dWB (see and the for details). For panels B through H, N = 6 biological replicates per group and data are represented as mean (standard deviation), except panel C, which is a visualization of the frequency. rCryo:dWB and rFibCon:dWB were compared using an unpaired Student t test (Mann-Whitney U test). ∗∗ P < .01; ∗ P < .05.

Article Snippet: Hyperfibrinolytic coagulopathy was not well resuscitated with adjuncts under flow, especially at higher shear rates, in contrast to static assays, in which Cryo rescued lysis better than FibCon (LI60, ROTEM EXTEM ( ; ), consistent with previous reports.

Techniques: In Vitro, Shear, Lysis, Standard Deviation, MANN-WHITNEY

Journal: Blood Vessels, Thrombosis & Hemostasis

Article Title: Platelet recruitment kinetics are impacted by von Willebrand factor quality in hemostatic adjuncts

doi: 10.1016/j.bvth.2025.100076

Figure Lengend Snippet: Cryo and FibCon both fail to rescue in vitro–induced lytic coagulopathy under physiological flow conditions. (A) Representative phase images of clot formation in the injury site over the course of perfusion experiments in the microfluidic model of bleeding. (B) BTs, (C) closure frequency (the percentage of experiments that achieved complete closure), and (D) terminal clot surface area for rCryo:lyWB and rFibCon:lyWB at all 3 shear rates. In panel B, dotted line indicates assay cutoff at 1200 seconds and is representative of no occlusion. (E) Lag-phase duration, (F) growth rates, (G) clot lysis duration, and (H) number of total independently identified events extracted from kinetic analyses of clot formation in rCryo:lyWB and rFibCon:lyWB (see and the for details). For panels B through H, N = 6 biological replicates per group and data are represented as mean (standard deviation), except panel C, which is a visualization of the frequency. rCryo:lyWB and rFibCon:lyWB were compared using an unpaired Student t test (Mann-Whitney U test).

Article Snippet: Hyperfibrinolytic coagulopathy was not well resuscitated with adjuncts under flow, especially at higher shear rates, in contrast to static assays, in which Cryo rescued lysis better than FibCon (LI60, ROTEM EXTEM ( ; ), consistent with previous reports.

Techniques: In Vitro, Shear, Lysis, Standard Deviation, MANN-WHITNEY