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MedChemExpress mitochondrial membrane potential assay mitochondrial membrane potential
Mitochondrial Membrane Potential Assay Mitochondrial Membrane Potential, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ALPCO mouse serum ferritin elisa kit
Pharmacological CB2R activation limits hepatic iron accumulation by modulating the hepcidin-FPN1 axis. a Representative Prussian blue staining of liver sections from pharmacological treatment groups (black arrows indicate positive blue iron deposits; scale bars = 100 and 20 µm). b Liver iron concentrations quantified using a ferrozine-based assay ( n = 4). c <t>Serum</t> <t>ferritin</t> levels determined using <t>ELISA</t> ( n = 5). d Serum hepcidin levels using ELISA ( n = 6). e Hepatic HAMP mRNA expression determined by qRT-PCR ( n = 5). f Representative Western blots of iron-related proteins FPN1, FTH, and FTL in the liver across different treatment groups, with β-actin as the loading control. * P < 0.05, ** P < 0.01, *** P < 0.001. Data are presented as the mean ± SD for each group. Individual data points are displayed as dots
Mouse Serum Ferritin Elisa Kit, supplied by ALPCO, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pharmacological CB2R activation limits hepatic iron accumulation by modulating the hepcidin-FPN1 axis. a Representative Prussian blue staining of liver sections from pharmacological treatment groups (black arrows indicate positive blue iron deposits; scale bars = 100 and 20 µm). b Liver iron concentrations quantified using a ferrozine-based assay ( n = 4). c <t>Serum</t> <t>ferritin</t> levels determined using <t>ELISA</t> ( n = 5). d Serum hepcidin levels using ELISA ( n = 6). e Hepatic HAMP mRNA expression determined by qRT-PCR ( n = 5). f Representative Western blots of iron-related proteins FPN1, FTH, and FTL in the liver across different treatment groups, with β-actin as the loading control. * P < 0.05, ** P < 0.01, *** P < 0.001. Data are presented as the mean ± SD for each group. Individual data points are displayed as dots
Ferritin, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology ferritin
Pharmacological CB2R activation limits hepatic iron accumulation by modulating the hepcidin-FPN1 axis. a Representative Prussian blue staining of liver sections from pharmacological treatment groups (black arrows indicate positive blue iron deposits; scale bars = 100 and 20 µm). b Liver iron concentrations quantified using a ferrozine-based assay ( n = 4). c <t>Serum</t> <t>ferritin</t> levels determined using <t>ELISA</t> ( n = 5). d Serum hepcidin levels using ELISA ( n = 6). e Hepatic HAMP mRNA expression determined by qRT-PCR ( n = 5). f Representative Western blots of iron-related proteins FPN1, FTH, and FTL in the liver across different treatment groups, with β-actin as the loading control. * P < 0.05, ** P < 0.01, *** P < 0.001. Data are presented as the mean ± SD for each group. Individual data points are displayed as dots
Ferritin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cormay Inc ferritin
Pharmacological CB2R activation limits hepatic iron accumulation by modulating the hepcidin-FPN1 axis. a Representative Prussian blue staining of liver sections from pharmacological treatment groups (black arrows indicate positive blue iron deposits; scale bars = 100 and 20 µm). b Liver iron concentrations quantified using a ferrozine-based assay ( n = 4). c <t>Serum</t> <t>ferritin</t> levels determined using <t>ELISA</t> ( n = 5). d Serum hepcidin levels using ELISA ( n = 6). e Hepatic HAMP mRNA expression determined by qRT-PCR ( n = 5). f Representative Western blots of iron-related proteins FPN1, FTH, and FTL in the liver across different treatment groups, with β-actin as the loading control. * P < 0.05, ** P < 0.01, *** P < 0.001. Data are presented as the mean ± SD for each group. Individual data points are displayed as dots
Ferritin, supplied by Cormay Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology ferritin
Pharmacological CB2R activation limits hepatic iron accumulation by modulating the hepcidin-FPN1 axis. a Representative Prussian blue staining of liver sections from pharmacological treatment groups (black arrows indicate positive blue iron deposits; scale bars = 100 and 20 µm). b Liver iron concentrations quantified using a ferrozine-based assay ( n = 4). c <t>Serum</t> <t>ferritin</t> levels determined using <t>ELISA</t> ( n = 5). d Serum hepcidin levels using ELISA ( n = 6). e Hepatic HAMP mRNA expression determined by qRT-PCR ( n = 5). f Representative Western blots of iron-related proteins FPN1, FTH, and FTL in the liver across different treatment groups, with β-actin as the loading control. * P < 0.05, ** P < 0.01, *** P < 0.001. Data are presented as the mean ± SD for each group. Individual data points are displayed as dots
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Musashi Engineering Inc isoform fthl17c ferritin
Pharmacological CB2R activation limits hepatic iron accumulation by modulating the hepcidin-FPN1 axis. a Representative Prussian blue staining of liver sections from pharmacological treatment groups (black arrows indicate positive blue iron deposits; scale bars = 100 and 20 µm). b Liver iron concentrations quantified using a ferrozine-based assay ( n = 4). c <t>Serum</t> <t>ferritin</t> levels determined using <t>ELISA</t> ( n = 5). d Serum hepcidin levels using ELISA ( n = 6). e Hepatic HAMP mRNA expression determined by qRT-PCR ( n = 5). f Representative Western blots of iron-related proteins FPN1, FTH, and FTL in the liver across different treatment groups, with β-actin as the loading control. * P < 0.05, ** P < 0.01, *** P < 0.001. Data are presented as the mean ± SD for each group. Individual data points are displayed as dots
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Cusabio enzyme linked immunosorbent assay kit
Pharmacological CB2R activation limits hepatic iron accumulation by modulating the hepcidin-FPN1 axis. a Representative Prussian blue staining of liver sections from pharmacological treatment groups (black arrows indicate positive blue iron deposits; scale bars = 100 and 20 µm). b Liver iron concentrations quantified using a ferrozine-based assay ( n = 4). c <t>Serum</t> <t>ferritin</t> levels determined using <t>ELISA</t> ( n = 5). d Serum hepcidin levels using ELISA ( n = 6). e Hepatic HAMP mRNA expression determined by qRT-PCR ( n = 5). f Representative Western blots of iron-related proteins FPN1, FTH, and FTL in the liver across different treatment groups, with β-actin as the loading control. * P < 0.05, ** P < 0.01, *** P < 0.001. Data are presented as the mean ± SD for each group. Individual data points are displayed as dots
Enzyme Linked Immunosorbent Assay Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MedChemExpress mitochondrial membrane potential δψm
LT-NPs-NIR attenuate oxidative stress, preserve <t>mitochondrial</t> integrity, and suppress senescence in TSPCs by inhibiting the mtDNA-STING-NF-κB axis. (A) Schematic of the experimental design. (B) TSPC proliferation assessed by CCK-8 assay. (C, E) Immunofluorescence staining and quantification of HSP70 (n = 3). (D, F) Mitochondrial membrane potential <t>(ΔΨm)</t> visualized by JC-1 staining (red: high potential; green: low potential) and quantification. (G) Multi-SIM of mtDNA (magenta) and TOMM20 (green) with colocalization analysis. (H) Western blot of cGAS-STING-IRF3-NF-κB pathway proteins. (I, K) Colony-forming unit fibroblast (CFU-F) assay and quantification of self-renewal capacity (n = 3). (J) Senescence-associated β-galactosidase (SA-β-gal) activity. (L, M) Apoptosis analysis by Annexin V/PI flow cytometry and quantification (n = 3). Scale bars: 100 μm (C); 5 μm (D, G); 200 μm (J). Significance: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
Mitochondrial Membrane Potential δψm, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MedChemExpress mitochondrial antioxidant mitotempo
LT-NPs-NIR attenuate oxidative stress, preserve <t>mitochondrial</t> integrity, and suppress senescence in TSPCs by inhibiting the mtDNA-STING-NF-κB axis. (A) Schematic of the experimental design. (B) TSPC proliferation assessed by CCK-8 assay. (C, E) Immunofluorescence staining and quantification of HSP70 (n = 3). (D, F) Mitochondrial membrane potential <t>(ΔΨm)</t> visualized by JC-1 staining (red: high potential; green: low potential) and quantification. (G) Multi-SIM of mtDNA (magenta) and TOMM20 (green) with colocalization analysis. (H) Western blot of cGAS-STING-IRF3-NF-κB pathway proteins. (I, K) Colony-forming unit fibroblast (CFU-F) assay and quantification of self-renewal capacity (n = 3). (J) Senescence-associated β-galactosidase (SA-β-gal) activity. (L, M) Apoptosis analysis by Annexin V/PI flow cytometry and quantification (n = 3). Scale bars: 100 μm (C); 5 μm (D, G); 200 μm (J). Significance: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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Pharmacological CB2R activation limits hepatic iron accumulation by modulating the hepcidin-FPN1 axis. a Representative Prussian blue staining of liver sections from pharmacological treatment groups (black arrows indicate positive blue iron deposits; scale bars = 100 and 20 µm). b Liver iron concentrations quantified using a ferrozine-based assay ( n = 4). c Serum ferritin levels determined using ELISA ( n = 5). d Serum hepcidin levels using ELISA ( n = 6). e Hepatic HAMP mRNA expression determined by qRT-PCR ( n = 5). f Representative Western blots of iron-related proteins FPN1, FTH, and FTL in the liver across different treatment groups, with β-actin as the loading control. * P < 0.05, ** P < 0.01, *** P < 0.001. Data are presented as the mean ± SD for each group. Individual data points are displayed as dots

Journal: Molecular Biomedicine

Article Title: Activating Cannabinoid receptor 2 alleviates iron overload-induced liver damage via dual modulation of STAT3/hepcidin and Nrf2/FPN1 pathways

doi: 10.1186/s43556-026-00541-1

Figure Lengend Snippet: Pharmacological CB2R activation limits hepatic iron accumulation by modulating the hepcidin-FPN1 axis. a Representative Prussian blue staining of liver sections from pharmacological treatment groups (black arrows indicate positive blue iron deposits; scale bars = 100 and 20 µm). b Liver iron concentrations quantified using a ferrozine-based assay ( n = 4). c Serum ferritin levels determined using ELISA ( n = 5). d Serum hepcidin levels using ELISA ( n = 6). e Hepatic HAMP mRNA expression determined by qRT-PCR ( n = 5). f Representative Western blots of iron-related proteins FPN1, FTH, and FTL in the liver across different treatment groups, with β-actin as the loading control. * P < 0.05, ** P < 0.01, *** P < 0.001. Data are presented as the mean ± SD for each group. Individual data points are displayed as dots

Article Snippet: The mouse serum Ferritin elisa kit (41-FERMS-E01) was purchased from ALPCO (Beijing, China).

Techniques: Activation Assay, Staining, Ferrozine Assay, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot, Control

LT-NPs-NIR attenuate oxidative stress, preserve mitochondrial integrity, and suppress senescence in TSPCs by inhibiting the mtDNA-STING-NF-κB axis. (A) Schematic of the experimental design. (B) TSPC proliferation assessed by CCK-8 assay. (C, E) Immunofluorescence staining and quantification of HSP70 (n = 3). (D, F) Mitochondrial membrane potential (ΔΨm) visualized by JC-1 staining (red: high potential; green: low potential) and quantification. (G) Multi-SIM of mtDNA (magenta) and TOMM20 (green) with colocalization analysis. (H) Western blot of cGAS-STING-IRF3-NF-κB pathway proteins. (I, K) Colony-forming unit fibroblast (CFU-F) assay and quantification of self-renewal capacity (n = 3). (J) Senescence-associated β-galactosidase (SA-β-gal) activity. (L, M) Apoptosis analysis by Annexin V/PI flow cytometry and quantification (n = 3). Scale bars: 100 μm (C); 5 μm (D, G); 200 μm (J). Significance: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Journal: Bioactive Materials

Article Title: On-demand mild photothermal cascade platform reprogramming mitochondrial immunity for tendon rejuvenation

doi: 10.1016/j.bioactmat.2026.01.004

Figure Lengend Snippet: LT-NPs-NIR attenuate oxidative stress, preserve mitochondrial integrity, and suppress senescence in TSPCs by inhibiting the mtDNA-STING-NF-κB axis. (A) Schematic of the experimental design. (B) TSPC proliferation assessed by CCK-8 assay. (C, E) Immunofluorescence staining and quantification of HSP70 (n = 3). (D, F) Mitochondrial membrane potential (ΔΨm) visualized by JC-1 staining (red: high potential; green: low potential) and quantification. (G) Multi-SIM of mtDNA (magenta) and TOMM20 (green) with colocalization analysis. (H) Western blot of cGAS-STING-IRF3-NF-κB pathway proteins. (I, K) Colony-forming unit fibroblast (CFU-F) assay and quantification of self-renewal capacity (n = 3). (J) Senescence-associated β-galactosidase (SA-β-gal) activity. (L, M) Apoptosis analysis by Annexin V/PI flow cytometry and quantification (n = 3). Scale bars: 100 μm (C); 5 μm (D, G); 200 μm (J). Significance: ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: Mitochondrial membrane potential (ΔΨm) was evaluated via JC-1 staining (MedChemExpress, USA).

Techniques: CCK-8 Assay, Immunofluorescence, Staining, Membrane, Western Blot, Activity Assay, Flow Cytometry