Journal: PLoS ONE

Article Title: An inter-island comparison of Darwin’s finches reveals the impact of habitat, host phylogeny, and island on the gut microbiome

doi: 10.1371/journal.pone.0226432

Figure Lengend Snippet: A) Phylogeny of Darwin’s finch species on Floreana Island based on whole-genome resequencing and the mean relative abundance of bacterial phyla across all gut microbiome samples of each species. Species abbreviations are given in . (Note: the hybrid cluster was not represented in the whole-genome resequencing dataset and therefore lacks a branch on this phylogeny). B) Relative abundance of the bacterial phyla in individual microbiome samples grouped according to species and habitat. The three nestlings are bracketed within the small ground finch lowland. Any bacterial phylum with mean relative abundance within a given finch species below 1% was omitted from both plots.

Article Snippet: To evaluate whether island of origin affects the gut microbiome composition of Darwin’s finches, data from this study were analyzed in conjunction with samples from Santa Cruz Island for the species that occur on both islands: small ground finch (Floreana: 25, Santa Cruz: 13), medium ground finch (F: 8, S: 6), cactus finch (F: 6, S: 6), and small tree finch (F: 14, S: 9), totaling 87 fecal microbiome samples [ ]; ).

Techniques:

Journal: PLoS ONE

Article Title: An inter-island comparison of Darwin’s finches reveals the impact of habitat, host phylogeny, and island on the gut microbiome

doi: 10.1371/journal.pone.0226432

Figure Lengend Snippet: A) Gut microbiome samples from Floreana are plotted on the first two principal coordinate axes, with point color and point shape indicating host species and habitat, respectively. Overall a separation between highland and lowland samples can be seen along the first axis, with highland samples and lowland samples mostly to the left and right, respectively. B) Individual amplicon sequence variants (ASVs) are plotted in the same ordination space as the gut microbiome samples. Bacterial phyla with at least 1% relative abundance across samples are color-coded; all other ASVs are gray. The demarcation between highland and lowland samples is recapitulated by the ASVs, with Proteobacteria and Actinobacteria corresponding to highland (left) and lowland (right) samples, respectively.

Article Snippet: To evaluate whether island of origin affects the gut microbiome composition of Darwin’s finches, data from this study were analyzed in conjunction with samples from Santa Cruz Island for the species that occur on both islands: small ground finch (Floreana: 25, Santa Cruz: 13), medium ground finch (F: 8, S: 6), cactus finch (F: 6, S: 6), and small tree finch (F: 14, S: 9), totaling 87 fecal microbiome samples [ ]; ).

Techniques: Amplification, Sequencing

Journal: PLoS ONE

Article Title: An inter-island comparison of Darwin’s finches reveals the impact of habitat, host phylogeny, and island on the gut microbiome

doi: 10.1371/journal.pone.0226432

Figure Lengend Snippet: Point color and shape indicate host species and habitat, respectively. A) Individual δ 13 C and δ 15 N values for each finch with gut microbiome samples. B) Mean δ 13 C and δ 15 N values for each species and habitat with standard deviation. The ground finch species (SGF, MGF, and CF) all had at least one sample in both habitats and have distinct δ 13 C and δ 15 N values dependent on the habitat of origin.

Article Snippet: To evaluate whether island of origin affects the gut microbiome composition of Darwin’s finches, data from this study were analyzed in conjunction with samples from Santa Cruz Island for the species that occur on both islands: small ground finch (Floreana: 25, Santa Cruz: 13), medium ground finch (F: 8, S: 6), cactus finch (F: 6, S: 6), and small tree finch (F: 14, S: 9), totaling 87 fecal microbiome samples [ ]; ).

Techniques: Standard Deviation

Journal: PLoS ONE

Article Title: An inter-island comparison of Darwin’s finches reveals the impact of habitat, host phylogeny, and island on the gut microbiome

doi: 10.1371/journal.pone.0226432

Figure Lengend Snippet: Results of variation partitioning using weighted UniFrac distances between gut microbiome samples against Darwin’s finch phylogeny (first two principal coordinate axes), stable isotope values ( δ 13 C and δ 15 N values), and foraging data (first two principal component axes) visualized with a Venn diagram. Adjusted R 2 values for each component are plotted inside the circles. All testable components include the p-value calculated using distance based redundancy analysis. Adjusted R 2 values in [a], [b], and [c] are the amount of variation explained uniquely by the corresponding explanatory table. Parts [d], [e], and [f] are amounts of variation that can be explained by either table in the overlap and part [g] is shared by all three tables. Foraging data is the only table with a positive adjusted R 2 value after controlling for overlapping variance.

Article Snippet: To evaluate whether island of origin affects the gut microbiome composition of Darwin’s finches, data from this study were analyzed in conjunction with samples from Santa Cruz Island for the species that occur on both islands: small ground finch (Floreana: 25, Santa Cruz: 13), medium ground finch (F: 8, S: 6), cactus finch (F: 6, S: 6), and small tree finch (F: 14, S: 9), totaling 87 fecal microbiome samples [ ]; ).

Techniques: