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Figure 6 The panel shows representative images of H9C2 cell stained by dihydroethidium (DHE) to determine ROS content (A). Quantitative analyses of DHE fluorescence intensities are shown on the right (B). MDA (C), GSH (D) and SOD (E) expression in H9C2 cell of OGD/R. Level of <t>Fe2+</t> (F) expression in H9C2 cell of OGD/R.Western blot detected the expression of related proteins (G). The quantification of the expression of related proteins (H–J). The data are presented as the mean±SD, n=3–5. *p<0.05, **p<0.01, ***p<0.001 and ****p<0.0001.
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Figure 6 The panel shows representative images of H9C2 cell stained by dihydroethidium (DHE) to determine ROS content (A). Quantitative analyses of DHE fluorescence intensities are shown on the right (B). MDA (C), GSH (D) and SOD (E) expression in H9C2 cell of OGD/R. Level of Fe2+ (F) expression in H9C2 cell of OGD/R.Western blot detected the expression of related proteins (G). The quantification of the expression of related proteins (H–J). The data are presented as the mean±SD, n=3–5. *p<0.05, **p<0.01, ***p<0.001 and ****p<0.0001.

Journal: Journal of Inflammation Research

Article Title: Bergenin Alleviates Myocardial Ischemia/Reperfusion Injury via Regulating SIRT1 Through Ferroptosis

doi: 10.2147/jir.s497618

Figure Lengend Snippet: Figure 6 The panel shows representative images of H9C2 cell stained by dihydroethidium (DHE) to determine ROS content (A). Quantitative analyses of DHE fluorescence intensities are shown on the right (B). MDA (C), GSH (D) and SOD (E) expression in H9C2 cell of OGD/R. Level of Fe2+ (F) expression in H9C2 cell of OGD/R.Western blot detected the expression of related proteins (G). The quantification of the expression of related proteins (H–J). The data are presented as the mean±SD, n=3–5. *p<0.05, **p<0.01, ***p<0.001 and ****p<0.0001.

Article Snippet: The Measurement of Fe2+ Content Cell Ferrous Iron Colorimetric Assay Kit (Elabscience E-BC-K881-M) was used for the measurement of Fe2+ content.

Techniques: Staining, Fluorescence, Expressing, Western Blot