Journal: Renal Failure

Article Title: Salvianolic acid C alleviates acute kidney injury by restoring fructose-1,6-bisphosphatase 1-mediated gluconeogenesis

doi: 10.1080/0886022X.2026.2629902

Figure Lengend Snippet: SAC promotes renal gluconeogenesis in cisplatin-induced AKI. (A–C) qPCR analysis of Fbp1 , G6pc , and Pck1 . (D) Representative Western blot images showing protein expression levels of FBP1, G6PC, and PCK1. (E–G) Quantitative analysis of the Western blot results. The bar graphs represent the relative protein levels of FBP1, G6PC, and PCK1, calculated as the ratio of the integrated density (IntDen) of each target band to that of the corresponding β-actin band. Data are presented as mean ± SEM. (H,I) Serum glucose and lactate assessment of mice in Vehicle, SAC, Cis and Cis + SAC groups. (J,K) Renal glucose and lactate assessment of mice in Vehicle, SAC, Cis, and Cis + SAC groups. NS represents not significant. * p < 0.05. ** p < 0.01. *** p < 0.001.

Article Snippet: Sections were sequentially incubated with primary antibodies: LRP2 (Cat# GB112109 , Servicebio, Wuhan, Hubei, China), a marker localized to renal tubules, and FBP1 (Cat# 12842-1-AP, Proteintech, Rosemont, IL, USA), overnight at 4 °C.

Techniques: Western Blot, Expressing

Journal: Renal Failure

Article Title: Salvianolic acid C alleviates acute kidney injury by restoring fructose-1,6-bisphosphatase 1-mediated gluconeogenesis

doi: 10.1080/0886022X.2026.2629902

Figure Lengend Snippet: SAC protects HK2 cells from cisplatin-induced injury and rescues gluconeogenic pathway disruption in vitro . (A,B) qPCR analysis of Kim-1 , Ngal . (C) Representative Western blot images showing protein expression levels of KIM-1 and NGAL. (D,E) Quantitative analysis of the Western blot results. The bar graphs represent the relative protein levels of KIM-1 and NGAL, calculated as the ratio of the integrated density (IntDen) of each target band to that of the corresponding β-actin band. Data are presented as mean ± SEM. (F) Representative Western blot images showing protein expression levels of FBP1, G6PC, and PCK1. (G–I) Quantitative analysis of the Western blot results. The bar graphs represent the relative protein levels of FBP1, G6PC, and PCK1, calculated as the ratio of the integrated density (IntDen) of each target band to that of the corresponding β-actin band. Data are presented as mean ± SEM. (J,K) Lactate and glucose assessment of cell culture supernatant. NS represents not significant. * p < 0.05. ** p < 0.01. *** p < 0.001.

Article Snippet: Sections were sequentially incubated with primary antibodies: LRP2 (Cat# GB112109 , Servicebio, Wuhan, Hubei, China), a marker localized to renal tubules, and FBP1 (Cat# 12842-1-AP, Proteintech, Rosemont, IL, USA), overnight at 4 °C.

Techniques: Disruption, In Vitro, Western Blot, Expressing, Cell Culture

Journal: Renal Failure

Article Title: Salvianolic acid C alleviates acute kidney injury by restoring fructose-1,6-bisphosphatase 1-mediated gluconeogenesis

doi: 10.1080/0886022X.2026.2629902

Figure Lengend Snippet: FBP1 inhibition reverses the renoprotective effects of SAC in cisplatin-induced AKI. (A,B) Scr levels and BUN levels. (C) Representative photomicrographs of H&E staining, PAS staining and Cleaved-caspased-3 staining in renal tissues (original magnification ×20, ×40; Scale bar: 100 μm, 50 μm). (D) Quantitative assessment of tubular injury. (E) Quantitative assessment of PAS-positive area. (F) Quantitative assessment of cleaved-caspased-3 Area. (G–I) qPCR analysis of Kim-1 , Ngal , Fbp1 . (J) Representative Western blot images showing protein expression levels of FBP1 and NGAL. (K,L) Quantitative analysis of the Western blot results. The bar graphs represent the relative protein levels of FBP1 and NGAL, calculated as the ratio of the integrated density (IntDen) of each target band to that of the corresponding β-actin band. Data are presented as mean ± SEM. NS represents not significant. * p < 0.05. ** p < 0.01. *** p < 0.001.

Article Snippet: Sections were sequentially incubated with primary antibodies: LRP2 (Cat# GB112109 , Servicebio, Wuhan, Hubei, China), a marker localized to renal tubules, and FBP1 (Cat# 12842-1-AP, Proteintech, Rosemont, IL, USA), overnight at 4 °C.

Techniques: Inhibition, Staining, Western Blot, Expressing

Journal: Renal Failure

Article Title: Salvianolic acid C alleviates acute kidney injury by restoring fructose-1,6-bisphosphatase 1-mediated gluconeogenesis

doi: 10.1080/0886022X.2026.2629902

Figure Lengend Snippet: SAC promotes PGC1α/FBP1 axis in AKI mouse kidneys. (A) Representative images of multiplex immunofluorescence staining in renal tissues showing DAPI (blue, nuclei), LRP2 (green, proximal tubules), and FBP1 (red) (original magnification ×40; Scale bar: 50 μm). (B) Quantitative assessment of FBP1-positive area. (C) Representative photomicrographs of FOXO1 staining in renal tissues (original magnification ×40; Scale bar: 50 μm). (D) Quantitative assessment of FOXO1-positive cells. (E) Representative photomicrographs of PGC1α staining in renal tissues (original magnification ×40; Scale bar: 50 μm). (F) Quantitative assessment of PGC1α-positive Area. NS represents not significant. * p < 0.05. ** p < 0.01. *** p < 0.001.

Article Snippet: Sections were sequentially incubated with primary antibodies: LRP2 (Cat# GB112109 , Servicebio, Wuhan, Hubei, China), a marker localized to renal tubules, and FBP1 (Cat# 12842-1-AP, Proteintech, Rosemont, IL, USA), overnight at 4 °C.

Techniques: Multiplex Assay, Immunofluorescence, Staining

Journal: Renal Failure

Article Title: Salvianolic acid C alleviates acute kidney injury by restoring fructose-1,6-bisphosphatase 1-mediated gluconeogenesis

doi: 10.1080/0886022X.2026.2629902

Figure Lengend Snippet: SAC interacts with FBP1 (Uniprot: P09467 ). Three-dimensional structure of SAC-FBP1 docking is exhibited. (A) 2D structural map of SAC-FBP1 binding. (B) The predicted best mode of SAC and FBP1. (C) The intermolecular forces between SAC and FBP1 binding. (D) The ranking table of molecular docking model binding energies used for different binding sites. (E) SPR binding curve of the interaction between FBP1 protein and SAC.

Article Snippet: Sections were sequentially incubated with primary antibodies: LRP2 (Cat# GB112109 , Servicebio, Wuhan, Hubei, China), a marker localized to renal tubules, and FBP1 (Cat# 12842-1-AP, Proteintech, Rosemont, IL, USA), overnight at 4 °C.

Techniques: Binding Assay