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facs sorting  (AMS Biotechnology)
98
AMS Biotechnology facs sorting
(A-B) Generation of HLA-A2 <t>+</t> <t>Luciferase</t> + human oligodendrocytic cell line MO3.13-A2-Luc. (A-B) <t>FACS</t> analysis of the (A) HLA-A2 expression on wildtype (WT) MO3.13 cells (left), MO3.13-A2 cells 2 weeks after transfection with HLA-A2 expression vector (middle), and MO3.13-A2 cells after the FACS sorting for HLA-A2 (right). (B) GFP expression on MO3.13-A2-Luc cells after lentiviral transduction (left), GFP expression on FACS sorted MO3.13-A2-Luc cells (middle) and HLA-A2 expression on MO3.13-A2-Luc cells (right). Grey histograms represent the isotype control and untransduced cells for the analysis of HLA-A2 and GFP expression respectively. (C-E) Optimization of siRNA reverse transfection protocol for MO3.13-A2-Luc cells. (C) Real-time live cell imaging of MO3.13-A2-Luc cells transfected either with a non-targeting siRNA control (Scr) or with a siRNA cocktail targeting genes essential for cell survival (cell death siRNA, siCD). Transfected cells were imaged via the IncuCyte SX5 system for 72 h and real-time % cell confluency was quantified. (D) RT-qPCR analysis of PDL1 mRNA expression in MO3.13-A2-Luc cells transfected either with Scr siRNA or pool of 4 non-overlapping siRNAs targeting PDL1 (siCD274). Results are presented as fold change compared to the Scr after β-actin mRNA normalization. (E) FACS analysis of PD-L1 surface expression on MO3.13-A2-Luc cells transfected either with Scr or PD-L1 specific siRNA pool. Left: representative histograms indicating % PD-L1 expression, right: mean fluorescent intensity (MFI) of PD-L1 from 2 independent FACS data. (F) Phenotypic characterization of FluTC. The expression of CD4 and CD8 was determined on CD3 + FluTC, whereas the expression of effector and memory markers CD45RO and CD62L and co-inhibitory immune checkpoint molecules PD-1, LAG-3 and TIM-3 was determined on CD3 + CD8 + FluTC. (Tn: naïve T cells, Tcm: central memory T cells, Teff: terminal effector T cells, Tem: effector memory T cells). Grey histograms represent the isotype control. (G) Luciferase-based cytotoxicity assay to optimize the co-culture conditions for MO3.13-A2-Luc and FluTC. MO3.13-A2-Luc were pulsed with serial dilutions of the flu-peptide and co-cultured with FluTC. After 20 h of co-culture, remaining relative luciferase units (RLU) were measured. RLU measured in pulsed samples were normalized to RLU of unpulsed (no peptide) control. Statistical significance was calculated compared with no peptide control. (H) Selection of cytotoxicity and viability controls for the HTP screen. MO3.13-A2-Luc cells were transfected with the indicated siRNAs. After 72 h of transfection cells were pulsed with 0,01 µg/ml flu-peptide and co-cultured either with FluTC or treated with control T cell medium (CM). 20 h following co-culture, remaining RLU was measured. The RLU of each sample was normalized to the Scr1 control in the cytotoxicity (FluTC) or viability (CM) setting to determine the impact of gene knockdown. (A-G) Representative data of at least 2 independent experiments. (H) Cumulative data of three independent experiments each performed with four replicates per sample. (D, G-H) Graphs show mean +/- SD. p-values were calculated using two-tailed student’s t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001.
Facs Sorting, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+sorting/bio_rxiv__64898__2026__04__21__719872-204-26-20?v=AMS+Biotechnology
Average 98 stars, based on 1 article reviews
facs sorting - by Bioz Stars, 2026-07
98/100 stars
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fluorescence activated cell sorting facs apparatus  (Sony Biotechnology)
99
Sony Biotechnology fluorescence activated cell sorting facs apparatus
(A-B) Generation of HLA-A2 <t>+</t> <t>Luciferase</t> + human oligodendrocytic cell line MO3.13-A2-Luc. (A-B) <t>FACS</t> analysis of the (A) HLA-A2 expression on wildtype (WT) MO3.13 cells (left), MO3.13-A2 cells 2 weeks after transfection with HLA-A2 expression vector (middle), and MO3.13-A2 cells after the FACS sorting for HLA-A2 (right). (B) GFP expression on MO3.13-A2-Luc cells after lentiviral transduction (left), GFP expression on FACS sorted MO3.13-A2-Luc cells (middle) and HLA-A2 expression on MO3.13-A2-Luc cells (right). Grey histograms represent the isotype control and untransduced cells for the analysis of HLA-A2 and GFP expression respectively. (C-E) Optimization of siRNA reverse transfection protocol for MO3.13-A2-Luc cells. (C) Real-time live cell imaging of MO3.13-A2-Luc cells transfected either with a non-targeting siRNA control (Scr) or with a siRNA cocktail targeting genes essential for cell survival (cell death siRNA, siCD). Transfected cells were imaged via the IncuCyte SX5 system for 72 h and real-time % cell confluency was quantified. (D) RT-qPCR analysis of PDL1 mRNA expression in MO3.13-A2-Luc cells transfected either with Scr siRNA or pool of 4 non-overlapping siRNAs targeting PDL1 (siCD274). Results are presented as fold change compared to the Scr after β-actin mRNA normalization. (E) FACS analysis of PD-L1 surface expression on MO3.13-A2-Luc cells transfected either with Scr or PD-L1 specific siRNA pool. Left: representative histograms indicating % PD-L1 expression, right: mean fluorescent intensity (MFI) of PD-L1 from 2 independent FACS data. (F) Phenotypic characterization of FluTC. The expression of CD4 and CD8 was determined on CD3 + FluTC, whereas the expression of effector and memory markers CD45RO and CD62L and co-inhibitory immune checkpoint molecules PD-1, LAG-3 and TIM-3 was determined on CD3 + CD8 + FluTC. (Tn: naïve T cells, Tcm: central memory T cells, Teff: terminal effector T cells, Tem: effector memory T cells). Grey histograms represent the isotype control. (G) Luciferase-based cytotoxicity assay to optimize the co-culture conditions for MO3.13-A2-Luc and FluTC. MO3.13-A2-Luc were pulsed with serial dilutions of the flu-peptide and co-cultured with FluTC. After 20 h of co-culture, remaining relative luciferase units (RLU) were measured. RLU measured in pulsed samples were normalized to RLU of unpulsed (no peptide) control. Statistical significance was calculated compared with no peptide control. (H) Selection of cytotoxicity and viability controls for the HTP screen. MO3.13-A2-Luc cells were transfected with the indicated siRNAs. After 72 h of transfection cells were pulsed with 0,01 µg/ml flu-peptide and co-cultured either with FluTC or treated with control T cell medium (CM). 20 h following co-culture, remaining RLU was measured. The RLU of each sample was normalized to the Scr1 control in the cytotoxicity (FluTC) or viability (CM) setting to determine the impact of gene knockdown. (A-G) Representative data of at least 2 independent experiments. (H) Cumulative data of three independent experiments each performed with four replicates per sample. (D, G-H) Graphs show mean +/- SD. p-values were calculated using two-tailed student’s t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001.
Fluorescence Activated Cell Sorting Facs Apparatus, supplied by Sony Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+sorting/pm41956330-91-20-26?v=Sony+Biotechnology
Average 99 stars, based on 1 article reviews
fluorescence activated cell sorting facs apparatus - by Bioz Stars, 2026-07
99/100 stars
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fluorescence activated cell sorting facs buffer  (Moregate Exports Pty Ltd)
86
Moregate Exports Pty Ltd fluorescence activated cell sorting facs buffer
(A-B) Generation of HLA-A2 <t>+</t> <t>Luciferase</t> + human oligodendrocytic cell line MO3.13-A2-Luc. (A-B) <t>FACS</t> analysis of the (A) HLA-A2 expression on wildtype (WT) MO3.13 cells (left), MO3.13-A2 cells 2 weeks after transfection with HLA-A2 expression vector (middle), and MO3.13-A2 cells after the FACS sorting for HLA-A2 (right). (B) GFP expression on MO3.13-A2-Luc cells after lentiviral transduction (left), GFP expression on FACS sorted MO3.13-A2-Luc cells (middle) and HLA-A2 expression on MO3.13-A2-Luc cells (right). Grey histograms represent the isotype control and untransduced cells for the analysis of HLA-A2 and GFP expression respectively. (C-E) Optimization of siRNA reverse transfection protocol for MO3.13-A2-Luc cells. (C) Real-time live cell imaging of MO3.13-A2-Luc cells transfected either with a non-targeting siRNA control (Scr) or with a siRNA cocktail targeting genes essential for cell survival (cell death siRNA, siCD). Transfected cells were imaged via the IncuCyte SX5 system for 72 h and real-time % cell confluency was quantified. (D) RT-qPCR analysis of PDL1 mRNA expression in MO3.13-A2-Luc cells transfected either with Scr siRNA or pool of 4 non-overlapping siRNAs targeting PDL1 (siCD274). Results are presented as fold change compared to the Scr after β-actin mRNA normalization. (E) FACS analysis of PD-L1 surface expression on MO3.13-A2-Luc cells transfected either with Scr or PD-L1 specific siRNA pool. Left: representative histograms indicating % PD-L1 expression, right: mean fluorescent intensity (MFI) of PD-L1 from 2 independent FACS data. (F) Phenotypic characterization of FluTC. The expression of CD4 and CD8 was determined on CD3 + FluTC, whereas the expression of effector and memory markers CD45RO and CD62L and co-inhibitory immune checkpoint molecules PD-1, LAG-3 and TIM-3 was determined on CD3 + CD8 + FluTC. (Tn: naïve T cells, Tcm: central memory T cells, Teff: terminal effector T cells, Tem: effector memory T cells). Grey histograms represent the isotype control. (G) Luciferase-based cytotoxicity assay to optimize the co-culture conditions for MO3.13-A2-Luc and FluTC. MO3.13-A2-Luc were pulsed with serial dilutions of the flu-peptide and co-cultured with FluTC. After 20 h of co-culture, remaining relative luciferase units (RLU) were measured. RLU measured in pulsed samples were normalized to RLU of unpulsed (no peptide) control. Statistical significance was calculated compared with no peptide control. (H) Selection of cytotoxicity and viability controls for the HTP screen. MO3.13-A2-Luc cells were transfected with the indicated siRNAs. After 72 h of transfection cells were pulsed with 0,01 µg/ml flu-peptide and co-cultured either with FluTC or treated with control T cell medium (CM). 20 h following co-culture, remaining RLU was measured. The RLU of each sample was normalized to the Scr1 control in the cytotoxicity (FluTC) or viability (CM) setting to determine the impact of gene knockdown. (A-G) Representative data of at least 2 independent experiments. (H) Cumulative data of three independent experiments each performed with four replicates per sample. (D, G-H) Graphs show mean +/- SD. p-values were calculated using two-tailed student’s t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001.
Fluorescence Activated Cell Sorting Facs Buffer, supplied by Moregate Exports Pty Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+sorting/pm42319180-93-16-27?v=Moregate+Exports+Pty+Ltd
Average 86 stars, based on 1 article reviews
fluorescence activated cell sorting facs buffer - by Bioz Stars, 2026-07
86/100 stars
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fluorescence activated cell sorting facs  (Biomark Inc)
86
Biomark Inc fluorescence activated cell sorting facs
(A-B) Generation of HLA-A2 <t>+</t> <t>Luciferase</t> + human oligodendrocytic cell line MO3.13-A2-Luc. (A-B) <t>FACS</t> analysis of the (A) HLA-A2 expression on wildtype (WT) MO3.13 cells (left), MO3.13-A2 cells 2 weeks after transfection with HLA-A2 expression vector (middle), and MO3.13-A2 cells after the FACS sorting for HLA-A2 (right). (B) GFP expression on MO3.13-A2-Luc cells after lentiviral transduction (left), GFP expression on FACS sorted MO3.13-A2-Luc cells (middle) and HLA-A2 expression on MO3.13-A2-Luc cells (right). Grey histograms represent the isotype control and untransduced cells for the analysis of HLA-A2 and GFP expression respectively. (C-E) Optimization of siRNA reverse transfection protocol for MO3.13-A2-Luc cells. (C) Real-time live cell imaging of MO3.13-A2-Luc cells transfected either with a non-targeting siRNA control (Scr) or with a siRNA cocktail targeting genes essential for cell survival (cell death siRNA, siCD). Transfected cells were imaged via the IncuCyte SX5 system for 72 h and real-time % cell confluency was quantified. (D) RT-qPCR analysis of PDL1 mRNA expression in MO3.13-A2-Luc cells transfected either with Scr siRNA or pool of 4 non-overlapping siRNAs targeting PDL1 (siCD274). Results are presented as fold change compared to the Scr after β-actin mRNA normalization. (E) FACS analysis of PD-L1 surface expression on MO3.13-A2-Luc cells transfected either with Scr or PD-L1 specific siRNA pool. Left: representative histograms indicating % PD-L1 expression, right: mean fluorescent intensity (MFI) of PD-L1 from 2 independent FACS data. (F) Phenotypic characterization of FluTC. The expression of CD4 and CD8 was determined on CD3 + FluTC, whereas the expression of effector and memory markers CD45RO and CD62L and co-inhibitory immune checkpoint molecules PD-1, LAG-3 and TIM-3 was determined on CD3 + CD8 + FluTC. (Tn: naïve T cells, Tcm: central memory T cells, Teff: terminal effector T cells, Tem: effector memory T cells). Grey histograms represent the isotype control. (G) Luciferase-based cytotoxicity assay to optimize the co-culture conditions for MO3.13-A2-Luc and FluTC. MO3.13-A2-Luc were pulsed with serial dilutions of the flu-peptide and co-cultured with FluTC. After 20 h of co-culture, remaining relative luciferase units (RLU) were measured. RLU measured in pulsed samples were normalized to RLU of unpulsed (no peptide) control. Statistical significance was calculated compared with no peptide control. (H) Selection of cytotoxicity and viability controls for the HTP screen. MO3.13-A2-Luc cells were transfected with the indicated siRNAs. After 72 h of transfection cells were pulsed with 0,01 µg/ml flu-peptide and co-cultured either with FluTC or treated with control T cell medium (CM). 20 h following co-culture, remaining RLU was measured. The RLU of each sample was normalized to the Scr1 control in the cytotoxicity (FluTC) or viability (CM) setting to determine the impact of gene knockdown. (A-G) Representative data of at least 2 independent experiments. (H) Cumulative data of three independent experiments each performed with four replicates per sample. (D, G-H) Graphs show mean +/- SD. p-values were calculated using two-tailed student’s t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001.
Fluorescence Activated Cell Sorting Facs, supplied by Biomark Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+sorting/pmc13114128-29-33-42?v=Biomark+Inc
Average 86 stars, based on 1 article reviews
fluorescence activated cell sorting facs - by Bioz Stars, 2026-07
86/100 stars
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facs pinsgfp entpd3 sorting pinsgfp clusters  (Eppendorf AG)
93
Eppendorf AG facs pinsgfp entpd3 sorting pinsgfp clusters
(A-B) Generation of HLA-A2 <t>+</t> <t>Luciferase</t> + human oligodendrocytic cell line MO3.13-A2-Luc. (A-B) <t>FACS</t> analysis of the (A) HLA-A2 expression on wildtype (WT) MO3.13 cells (left), MO3.13-A2 cells 2 weeks after transfection with HLA-A2 expression vector (middle), and MO3.13-A2 cells after the FACS sorting for HLA-A2 (right). (B) GFP expression on MO3.13-A2-Luc cells after lentiviral transduction (left), GFP expression on FACS sorted MO3.13-A2-Luc cells (middle) and HLA-A2 expression on MO3.13-A2-Luc cells (right). Grey histograms represent the isotype control and untransduced cells for the analysis of HLA-A2 and GFP expression respectively. (C-E) Optimization of siRNA reverse transfection protocol for MO3.13-A2-Luc cells. (C) Real-time live cell imaging of MO3.13-A2-Luc cells transfected either with a non-targeting siRNA control (Scr) or with a siRNA cocktail targeting genes essential for cell survival (cell death siRNA, siCD). Transfected cells were imaged via the IncuCyte SX5 system for 72 h and real-time % cell confluency was quantified. (D) RT-qPCR analysis of PDL1 mRNA expression in MO3.13-A2-Luc cells transfected either with Scr siRNA or pool of 4 non-overlapping siRNAs targeting PDL1 (siCD274). Results are presented as fold change compared to the Scr after β-actin mRNA normalization. (E) FACS analysis of PD-L1 surface expression on MO3.13-A2-Luc cells transfected either with Scr or PD-L1 specific siRNA pool. Left: representative histograms indicating % PD-L1 expression, right: mean fluorescent intensity (MFI) of PD-L1 from 2 independent FACS data. (F) Phenotypic characterization of FluTC. The expression of CD4 and CD8 was determined on CD3 + FluTC, whereas the expression of effector and memory markers CD45RO and CD62L and co-inhibitory immune checkpoint molecules PD-1, LAG-3 and TIM-3 was determined on CD3 + CD8 + FluTC. (Tn: naïve T cells, Tcm: central memory T cells, Teff: terminal effector T cells, Tem: effector memory T cells). Grey histograms represent the isotype control. (G) Luciferase-based cytotoxicity assay to optimize the co-culture conditions for MO3.13-A2-Luc and FluTC. MO3.13-A2-Luc were pulsed with serial dilutions of the flu-peptide and co-cultured with FluTC. After 20 h of co-culture, remaining relative luciferase units (RLU) were measured. RLU measured in pulsed samples were normalized to RLU of unpulsed (no peptide) control. Statistical significance was calculated compared with no peptide control. (H) Selection of cytotoxicity and viability controls for the HTP screen. MO3.13-A2-Luc cells were transfected with the indicated siRNAs. After 72 h of transfection cells were pulsed with 0,01 µg/ml flu-peptide and co-cultured either with FluTC or treated with control T cell medium (CM). 20 h following co-culture, remaining RLU was measured. The RLU of each sample was normalized to the Scr1 control in the cytotoxicity (FluTC) or viability (CM) setting to determine the impact of gene knockdown. (A-G) Representative data of at least 2 independent experiments. (H) Cumulative data of three independent experiments each performed with four replicates per sample. (D, G-H) Graphs show mean +/- SD. p-values were calculated using two-tailed student’s t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001.
Facs Pinsgfp Entpd3 Sorting Pinsgfp Clusters, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+sorting/us12594306-246-4-13?v=Eppendorf+AG
Average 93 stars, based on 1 article reviews
facs pinsgfp entpd3 sorting pinsgfp clusters - by Bioz Stars, 2026-07
93/100 stars
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fluorescence activated cell sorting facs buffer  (Thermo Fisher)
99
Thermo Fisher fluorescence activated cell sorting facs buffer
(A-B) Generation of HLA-A2 <t>+</t> <t>Luciferase</t> + human oligodendrocytic cell line MO3.13-A2-Luc. (A-B) <t>FACS</t> analysis of the (A) HLA-A2 expression on wildtype (WT) MO3.13 cells (left), MO3.13-A2 cells 2 weeks after transfection with HLA-A2 expression vector (middle), and MO3.13-A2 cells after the FACS sorting for HLA-A2 (right). (B) GFP expression on MO3.13-A2-Luc cells after lentiviral transduction (left), GFP expression on FACS sorted MO3.13-A2-Luc cells (middle) and HLA-A2 expression on MO3.13-A2-Luc cells (right). Grey histograms represent the isotype control and untransduced cells for the analysis of HLA-A2 and GFP expression respectively. (C-E) Optimization of siRNA reverse transfection protocol for MO3.13-A2-Luc cells. (C) Real-time live cell imaging of MO3.13-A2-Luc cells transfected either with a non-targeting siRNA control (Scr) or with a siRNA cocktail targeting genes essential for cell survival (cell death siRNA, siCD). Transfected cells were imaged via the IncuCyte SX5 system for 72 h and real-time % cell confluency was quantified. (D) RT-qPCR analysis of PDL1 mRNA expression in MO3.13-A2-Luc cells transfected either with Scr siRNA or pool of 4 non-overlapping siRNAs targeting PDL1 (siCD274). Results are presented as fold change compared to the Scr after β-actin mRNA normalization. (E) FACS analysis of PD-L1 surface expression on MO3.13-A2-Luc cells transfected either with Scr or PD-L1 specific siRNA pool. Left: representative histograms indicating % PD-L1 expression, right: mean fluorescent intensity (MFI) of PD-L1 from 2 independent FACS data. (F) Phenotypic characterization of FluTC. The expression of CD4 and CD8 was determined on CD3 + FluTC, whereas the expression of effector and memory markers CD45RO and CD62L and co-inhibitory immune checkpoint molecules PD-1, LAG-3 and TIM-3 was determined on CD3 + CD8 + FluTC. (Tn: naïve T cells, Tcm: central memory T cells, Teff: terminal effector T cells, Tem: effector memory T cells). Grey histograms represent the isotype control. (G) Luciferase-based cytotoxicity assay to optimize the co-culture conditions for MO3.13-A2-Luc and FluTC. MO3.13-A2-Luc were pulsed with serial dilutions of the flu-peptide and co-cultured with FluTC. After 20 h of co-culture, remaining relative luciferase units (RLU) were measured. RLU measured in pulsed samples were normalized to RLU of unpulsed (no peptide) control. Statistical significance was calculated compared with no peptide control. (H) Selection of cytotoxicity and viability controls for the HTP screen. MO3.13-A2-Luc cells were transfected with the indicated siRNAs. After 72 h of transfection cells were pulsed with 0,01 µg/ml flu-peptide and co-cultured either with FluTC or treated with control T cell medium (CM). 20 h following co-culture, remaining RLU was measured. The RLU of each sample was normalized to the Scr1 control in the cytotoxicity (FluTC) or viability (CM) setting to determine the impact of gene knockdown. (A-G) Representative data of at least 2 independent experiments. (H) Cumulative data of three independent experiments each performed with four replicates per sample. (D, G-H) Graphs show mean +/- SD. p-values were calculated using two-tailed student’s t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001.
Fluorescence Activated Cell Sorting Facs Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+sorting/pm41917203-192-5-31?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
fluorescence activated cell sorting facs buffer - by Bioz Stars, 2026-07
99/100 stars
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facs sorting  (Sony Biotechnology)
99
Sony Biotechnology facs sorting
(A-B) Generation of HLA-A2 <t>+</t> <t>Luciferase</t> + human oligodendrocytic cell line MO3.13-A2-Luc. (A-B) <t>FACS</t> analysis of the (A) HLA-A2 expression on wildtype (WT) MO3.13 cells (left), MO3.13-A2 cells 2 weeks after transfection with HLA-A2 expression vector (middle), and MO3.13-A2 cells after the FACS sorting for HLA-A2 (right). (B) GFP expression on MO3.13-A2-Luc cells after lentiviral transduction (left), GFP expression on FACS sorted MO3.13-A2-Luc cells (middle) and HLA-A2 expression on MO3.13-A2-Luc cells (right). Grey histograms represent the isotype control and untransduced cells for the analysis of HLA-A2 and GFP expression respectively. (C-E) Optimization of siRNA reverse transfection protocol for MO3.13-A2-Luc cells. (C) Real-time live cell imaging of MO3.13-A2-Luc cells transfected either with a non-targeting siRNA control (Scr) or with a siRNA cocktail targeting genes essential for cell survival (cell death siRNA, siCD). Transfected cells were imaged via the IncuCyte SX5 system for 72 h and real-time % cell confluency was quantified. (D) RT-qPCR analysis of PDL1 mRNA expression in MO3.13-A2-Luc cells transfected either with Scr siRNA or pool of 4 non-overlapping siRNAs targeting PDL1 (siCD274). Results are presented as fold change compared to the Scr after β-actin mRNA normalization. (E) FACS analysis of PD-L1 surface expression on MO3.13-A2-Luc cells transfected either with Scr or PD-L1 specific siRNA pool. Left: representative histograms indicating % PD-L1 expression, right: mean fluorescent intensity (MFI) of PD-L1 from 2 independent FACS data. (F) Phenotypic characterization of FluTC. The expression of CD4 and CD8 was determined on CD3 + FluTC, whereas the expression of effector and memory markers CD45RO and CD62L and co-inhibitory immune checkpoint molecules PD-1, LAG-3 and TIM-3 was determined on CD3 + CD8 + FluTC. (Tn: naïve T cells, Tcm: central memory T cells, Teff: terminal effector T cells, Tem: effector memory T cells). Grey histograms represent the isotype control. (G) Luciferase-based cytotoxicity assay to optimize the co-culture conditions for MO3.13-A2-Luc and FluTC. MO3.13-A2-Luc were pulsed with serial dilutions of the flu-peptide and co-cultured with FluTC. After 20 h of co-culture, remaining relative luciferase units (RLU) were measured. RLU measured in pulsed samples were normalized to RLU of unpulsed (no peptide) control. Statistical significance was calculated compared with no peptide control. (H) Selection of cytotoxicity and viability controls for the HTP screen. MO3.13-A2-Luc cells were transfected with the indicated siRNAs. After 72 h of transfection cells were pulsed with 0,01 µg/ml flu-peptide and co-cultured either with FluTC or treated with control T cell medium (CM). 20 h following co-culture, remaining RLU was measured. The RLU of each sample was normalized to the Scr1 control in the cytotoxicity (FluTC) or viability (CM) setting to determine the impact of gene knockdown. (A-G) Representative data of at least 2 independent experiments. (H) Cumulative data of three independent experiments each performed with four replicates per sample. (D, G-H) Graphs show mean +/- SD. p-values were calculated using two-tailed student’s t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001.
Facs Sorting, supplied by Sony Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+sorting/bio_rxiv__64898__2026__03__18__712348-183-19-21?v=Sony+Biotechnology
Average 99 stars, based on 1 article reviews
facs sorting - by Bioz Stars, 2026-07
99/100 stars
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fluorescence activated cell sorting facs flow cytometer  (Danaher Inc)
97
Danaher Inc fluorescence activated cell sorting facs flow cytometer
Effects of HC on the expression of proinflammatory macrophage (CD80) and anti-inflammatory macrophage (CD163) surface markers in RAW264.7 cells. A: Percentage of CD80(−)/CD163(−) (M0) macrophages. B: Percentage of CD80(+)/CD163(−) (M1) macrophages. C: Percentage of CD80(−)/CD163(+) (M2) macrophages. D: Representative <t>FACS</t> plots of each experimental group. The data are presented as the means ± SDs (n = 3 for each treatment group). ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0005, ∗∗∗∗ p < 0.0001; ns: not significant.
Fluorescence Activated Cell Sorting Facs Flow Cytometer, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facs+sorting/pmc12957811-144-15-23?v=Danaher+Inc
Average 97 stars, based on 1 article reviews
fluorescence activated cell sorting facs flow cytometer - by Bioz Stars, 2026-07
97/100 stars
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(A-B) Generation of HLA-A2 + Luciferase + human oligodendrocytic cell line MO3.13-A2-Luc. (A-B) FACS analysis of the (A) HLA-A2 expression on wildtype (WT) MO3.13 cells (left), MO3.13-A2 cells 2 weeks after transfection with HLA-A2 expression vector (middle), and MO3.13-A2 cells after the FACS sorting for HLA-A2 (right). (B) GFP expression on MO3.13-A2-Luc cells after lentiviral transduction (left), GFP expression on FACS sorted MO3.13-A2-Luc cells (middle) and HLA-A2 expression on MO3.13-A2-Luc cells (right). Grey histograms represent the isotype control and untransduced cells for the analysis of HLA-A2 and GFP expression respectively. (C-E) Optimization of siRNA reverse transfection protocol for MO3.13-A2-Luc cells. (C) Real-time live cell imaging of MO3.13-A2-Luc cells transfected either with a non-targeting siRNA control (Scr) or with a siRNA cocktail targeting genes essential for cell survival (cell death siRNA, siCD). Transfected cells were imaged via the IncuCyte SX5 system for 72 h and real-time % cell confluency was quantified. (D) RT-qPCR analysis of PDL1 mRNA expression in MO3.13-A2-Luc cells transfected either with Scr siRNA or pool of 4 non-overlapping siRNAs targeting PDL1 (siCD274). Results are presented as fold change compared to the Scr after β-actin mRNA normalization. (E) FACS analysis of PD-L1 surface expression on MO3.13-A2-Luc cells transfected either with Scr or PD-L1 specific siRNA pool. Left: representative histograms indicating % PD-L1 expression, right: mean fluorescent intensity (MFI) of PD-L1 from 2 independent FACS data. (F) Phenotypic characterization of FluTC. The expression of CD4 and CD8 was determined on CD3 + FluTC, whereas the expression of effector and memory markers CD45RO and CD62L and co-inhibitory immune checkpoint molecules PD-1, LAG-3 and TIM-3 was determined on CD3 + CD8 + FluTC. (Tn: naïve T cells, Tcm: central memory T cells, Teff: terminal effector T cells, Tem: effector memory T cells). Grey histograms represent the isotype control. (G) Luciferase-based cytotoxicity assay to optimize the co-culture conditions for MO3.13-A2-Luc and FluTC. MO3.13-A2-Luc were pulsed with serial dilutions of the flu-peptide and co-cultured with FluTC. After 20 h of co-culture, remaining relative luciferase units (RLU) were measured. RLU measured in pulsed samples were normalized to RLU of unpulsed (no peptide) control. Statistical significance was calculated compared with no peptide control. (H) Selection of cytotoxicity and viability controls for the HTP screen. MO3.13-A2-Luc cells were transfected with the indicated siRNAs. After 72 h of transfection cells were pulsed with 0,01 µg/ml flu-peptide and co-cultured either with FluTC or treated with control T cell medium (CM). 20 h following co-culture, remaining RLU was measured. The RLU of each sample was normalized to the Scr1 control in the cytotoxicity (FluTC) or viability (CM) setting to determine the impact of gene knockdown. (A-G) Representative data of at least 2 independent experiments. (H) Cumulative data of three independent experiments each performed with four replicates per sample. (D, G-H) Graphs show mean +/- SD. p-values were calculated using two-tailed student’s t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001.

Journal: bioRxiv

Article Title: Multifaceted immune resistance landscapes in human oligodendrocytes protect against cytotoxic T cells and are dysregulated in MS brain cell subsets

doi: 10.64898/2026.04.21.719872

Figure Lengend Snippet: (A-B) Generation of HLA-A2 + Luciferase + human oligodendrocytic cell line MO3.13-A2-Luc. (A-B) FACS analysis of the (A) HLA-A2 expression on wildtype (WT) MO3.13 cells (left), MO3.13-A2 cells 2 weeks after transfection with HLA-A2 expression vector (middle), and MO3.13-A2 cells after the FACS sorting for HLA-A2 (right). (B) GFP expression on MO3.13-A2-Luc cells after lentiviral transduction (left), GFP expression on FACS sorted MO3.13-A2-Luc cells (middle) and HLA-A2 expression on MO3.13-A2-Luc cells (right). Grey histograms represent the isotype control and untransduced cells for the analysis of HLA-A2 and GFP expression respectively. (C-E) Optimization of siRNA reverse transfection protocol for MO3.13-A2-Luc cells. (C) Real-time live cell imaging of MO3.13-A2-Luc cells transfected either with a non-targeting siRNA control (Scr) or with a siRNA cocktail targeting genes essential for cell survival (cell death siRNA, siCD). Transfected cells were imaged via the IncuCyte SX5 system for 72 h and real-time % cell confluency was quantified. (D) RT-qPCR analysis of PDL1 mRNA expression in MO3.13-A2-Luc cells transfected either with Scr siRNA or pool of 4 non-overlapping siRNAs targeting PDL1 (siCD274). Results are presented as fold change compared to the Scr after β-actin mRNA normalization. (E) FACS analysis of PD-L1 surface expression on MO3.13-A2-Luc cells transfected either with Scr or PD-L1 specific siRNA pool. Left: representative histograms indicating % PD-L1 expression, right: mean fluorescent intensity (MFI) of PD-L1 from 2 independent FACS data. (F) Phenotypic characterization of FluTC. The expression of CD4 and CD8 was determined on CD3 + FluTC, whereas the expression of effector and memory markers CD45RO and CD62L and co-inhibitory immune checkpoint molecules PD-1, LAG-3 and TIM-3 was determined on CD3 + CD8 + FluTC. (Tn: naïve T cells, Tcm: central memory T cells, Teff: terminal effector T cells, Tem: effector memory T cells). Grey histograms represent the isotype control. (G) Luciferase-based cytotoxicity assay to optimize the co-culture conditions for MO3.13-A2-Luc and FluTC. MO3.13-A2-Luc were pulsed with serial dilutions of the flu-peptide and co-cultured with FluTC. After 20 h of co-culture, remaining relative luciferase units (RLU) were measured. RLU measured in pulsed samples were normalized to RLU of unpulsed (no peptide) control. Statistical significance was calculated compared with no peptide control. (H) Selection of cytotoxicity and viability controls for the HTP screen. MO3.13-A2-Luc cells were transfected with the indicated siRNAs. After 72 h of transfection cells were pulsed with 0,01 µg/ml flu-peptide and co-cultured either with FluTC or treated with control T cell medium (CM). 20 h following co-culture, remaining RLU was measured. The RLU of each sample was normalized to the Scr1 control in the cytotoxicity (FluTC) or viability (CM) setting to determine the impact of gene knockdown. (A-G) Representative data of at least 2 independent experiments. (H) Cumulative data of three independent experiments each performed with four replicates per sample. (D, G-H) Graphs show mean +/- SD. p-values were calculated using two-tailed student’s t-test. * = p < 0.05, ** = p < 0.01, *** = p < 0.005, **** = p < 0.001.

Article Snippet: MO3.13-A2-Luc cells were generated after transduction of MO3.13-A2 cells with lentiviral particles encoding firefly luciferase and GFP (GenTarget Inc – Amsbio, #LVP020 (CMV-Luciferase (firefly)-2A-GFP (Puro)) and FACS sorting.

Techniques: Luciferase, Expressing, Transfection, Plasmid Preparation, Transduction, Control, Live Cell Imaging, Quantitative RT-PCR, Cytotoxicity Assay, Co-Culture Assay, Cell Culture, Selection, Knockdown, Two Tailed Test

Effects of HC on the expression of proinflammatory macrophage (CD80) and anti-inflammatory macrophage (CD163) surface markers in RAW264.7 cells. A: Percentage of CD80(−)/CD163(−) (M0) macrophages. B: Percentage of CD80(+)/CD163(−) (M1) macrophages. C: Percentage of CD80(−)/CD163(+) (M2) macrophages. D: Representative FACS plots of each experimental group. The data are presented as the means ± SDs (n = 3 for each treatment group). ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0005, ∗∗∗∗ p < 0.0001; ns: not significant.

Journal: Journal of Traditional and Complementary Medicine

Article Title: Anti-inflammatory and polarization-modulating effects of Houttuynia cordata in LPS-stimulated RAW264.7 macrophages

doi: 10.1016/j.jtcme.2025.05.001

Figure Lengend Snippet: Effects of HC on the expression of proinflammatory macrophage (CD80) and anti-inflammatory macrophage (CD163) surface markers in RAW264.7 cells. A: Percentage of CD80(−)/CD163(−) (M0) macrophages. B: Percentage of CD80(+)/CD163(−) (M1) macrophages. C: Percentage of CD80(−)/CD163(+) (M2) macrophages. D: Representative FACS plots of each experimental group. The data are presented as the means ± SDs (n = 3 for each treatment group). ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0005, ∗∗∗∗ p < 0.0001; ns: not significant.

Article Snippet: After the cells were fixed with 4 % paraformaldehyde, phagocytic uptake was analyzed via a Fluorescence-activated cell sorting (FACS) flow cytometer (CytoFLEX S, Backman, USA).

Techniques: Expressing