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Thermo Fisher gene exp fabp7 mm00445225 m1
Validation of the specificity of the ChAT-Cre/Ribotag mice. (a) RTlzqPCR revealed enrichment of cell type-specific transcripts in the IPs of ChAT and depletion of the glial transcript <t>Fabp7.</t> (b) TPM of motoneuron-specific genes expressed in the IP and I. (c) TPM of glial-specific genes in both the IP and I. (d) TPM of regeneration-specific genes expressed in the crush and control samples. (e, f) PCA of the crush and control samples included in the study.
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Validation of the specificity of the ChAT-Cre/Ribotag mice. (a) RTlzqPCR revealed enrichment of cell type-specific transcripts in the IPs of ChAT and depletion of the glial transcript <t>Fabp7.</t> (b) TPM of motoneuron-specific genes expressed in the IP and I. (c) TPM of glial-specific genes in both the IP and I. (d) TPM of regeneration-specific genes expressed in the crush and control samples. (e, f) PCA of the crush and control samples included in the study.
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Effects of chronic stress on the protein expression levels of <t>Fabp7</t> and Cxcl12 in the hippocampus and hypothalamus. (A) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hippocampus of the control and stress groups. Nuclei were counterstained with DAPI (blue). Scale bar: 100 μm, Zoom: 10 μm. (B, C) Quantification of the intensity and area of Fabp7 immunofluorescence in the hippocampus of the control and stress mice. (D) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (E, F) Quantification of the intensity and area of Fabp7 immunofluorescence in the hypothalamus of the control and stress groups. (G) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hippocampus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (H, I) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hippocampus of the control and stress groups. (J) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (K, L) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hypothalamus of the control and stress groups. All data are presented as mean ± SEM (n = 3–5 mice per group). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 stress vs control in adolescence or adolescence vs adulthood in the stress group (unpaired t -test).
Primary Antibodies Against Fabp7, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of chronic stress on the protein expression levels of <t>Fabp7</t> and Cxcl12 in the hippocampus and hypothalamus. (A) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hippocampus of the control and stress groups. Nuclei were counterstained with DAPI (blue). Scale bar: 100 μm, Zoom: 10 μm. (B, C) Quantification of the intensity and area of Fabp7 immunofluorescence in the hippocampus of the control and stress mice. (D) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (E, F) Quantification of the intensity and area of Fabp7 immunofluorescence in the hypothalamus of the control and stress groups. (G) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hippocampus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (H, I) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hippocampus of the control and stress groups. (J) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (K, L) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hypothalamus of the control and stress groups. All data are presented as mean ± SEM (n = 3–5 mice per group). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 stress vs control in adolescence or adolescence vs adulthood in the stress group (unpaired t -test).
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Effects of chronic stress on the protein expression levels of <t>Fabp7</t> and Cxcl12 in the hippocampus and hypothalamus. (A) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hippocampus of the control and stress groups. Nuclei were counterstained with DAPI (blue). Scale bar: 100 μm, Zoom: 10 μm. (B, C) Quantification of the intensity and area of Fabp7 immunofluorescence in the hippocampus of the control and stress mice. (D) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (E, F) Quantification of the intensity and area of Fabp7 immunofluorescence in the hypothalamus of the control and stress groups. (G) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hippocampus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (H, I) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hippocampus of the control and stress groups. (J) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (K, L) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hypothalamus of the control and stress groups. All data are presented as mean ± SEM (n = 3–5 mice per group). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 stress vs control in adolescence or adolescence vs adulthood in the stress group (unpaired t -test).
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Effects of chronic stress on the protein expression levels of <t>Fabp7</t> and Cxcl12 in the hippocampus and hypothalamus. (A) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hippocampus of the control and stress groups. Nuclei were counterstained with DAPI (blue). Scale bar: 100 μm, Zoom: 10 μm. (B, C) Quantification of the intensity and area of Fabp7 immunofluorescence in the hippocampus of the control and stress mice. (D) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (E, F) Quantification of the intensity and area of Fabp7 immunofluorescence in the hypothalamus of the control and stress groups. (G) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hippocampus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (H, I) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hippocampus of the control and stress groups. (J) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (K, L) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hypothalamus of the control and stress groups. All data are presented as mean ± SEM (n = 3–5 mice per group). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 stress vs control in adolescence or adolescence vs adulthood in the stress group (unpaired t -test).
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Effects of chronic stress on the protein expression levels of <t>Fabp7</t> and Cxcl12 in the hippocampus and hypothalamus. (A) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hippocampus of the control and stress groups. Nuclei were counterstained with DAPI (blue). Scale bar: 100 μm, Zoom: 10 μm. (B, C) Quantification of the intensity and area of Fabp7 immunofluorescence in the hippocampus of the control and stress mice. (D) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (E, F) Quantification of the intensity and area of Fabp7 immunofluorescence in the hypothalamus of the control and stress groups. (G) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hippocampus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (H, I) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hippocampus of the control and stress groups. (J) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (K, L) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hypothalamus of the control and stress groups. All data are presented as mean ± SEM (n = 3–5 mice per group). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 stress vs control in adolescence or adolescence vs adulthood in the stress group (unpaired t -test).
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Effects of chronic stress on the protein expression levels of <t>Fabp7</t> and Cxcl12 in the hippocampus and hypothalamus. (A) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hippocampus of the control and stress groups. Nuclei were counterstained with DAPI (blue). Scale bar: 100 μm, Zoom: 10 μm. (B, C) Quantification of the intensity and area of Fabp7 immunofluorescence in the hippocampus of the control and stress mice. (D) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (E, F) Quantification of the intensity and area of Fabp7 immunofluorescence in the hypothalamus of the control and stress groups. (G) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hippocampus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (H, I) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hippocampus of the control and stress groups. (J) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (K, L) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hypothalamus of the control and stress groups. All data are presented as mean ± SEM (n = 3–5 mice per group). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 stress vs control in adolescence or adolescence vs adulthood in the stress group (unpaired t -test).
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Image Search Results


Validation of the specificity of the ChAT-Cre/Ribotag mice. (a) RTlzqPCR revealed enrichment of cell type-specific transcripts in the IPs of ChAT and depletion of the glial transcript Fabp7. (b) TPM of motoneuron-specific genes expressed in the IP and I. (c) TPM of glial-specific genes in both the IP and I. (d) TPM of regeneration-specific genes expressed in the crush and control samples. (e, f) PCA of the crush and control samples included in the study.

Journal: bioRxiv

Article Title: Assessment of Motoneuronal Regeneration and Wallerian Degeneration Following Axotomy in Postnatal Mice

doi: 10.64898/2025.12.03.691768

Figure Lengend Snippet: Validation of the specificity of the ChAT-Cre/Ribotag mice. (a) RTlzqPCR revealed enrichment of cell type-specific transcripts in the IPs of ChAT and depletion of the glial transcript Fabp7. (b) TPM of motoneuron-specific genes expressed in the IP and I. (c) TPM of glial-specific genes in both the IP and I. (d) TPM of regeneration-specific genes expressed in the crush and control samples. (e, f) PCA of the crush and control samples included in the study.

Article Snippet: Specific transcripts were detected via TaqMan assays: Actb (Mm02619580_g1), Fabp7 (Mm00445225_m1), and Chat (Mm01221882_m1).

Techniques: Biomarker Discovery, Control

Effects of chronic stress on the protein expression levels of Fabp7 and Cxcl12 in the hippocampus and hypothalamus. (A) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hippocampus of the control and stress groups. Nuclei were counterstained with DAPI (blue). Scale bar: 100 μm, Zoom: 10 μm. (B, C) Quantification of the intensity and area of Fabp7 immunofluorescence in the hippocampus of the control and stress mice. (D) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (E, F) Quantification of the intensity and area of Fabp7 immunofluorescence in the hypothalamus of the control and stress groups. (G) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hippocampus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (H, I) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hippocampus of the control and stress groups. (J) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (K, L) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hypothalamus of the control and stress groups. All data are presented as mean ± SEM (n = 3–5 mice per group). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 stress vs control in adolescence or adolescence vs adulthood in the stress group (unpaired t -test).

Journal: Neurobiology of Stress

Article Title: Age-dependent effects of chronic traumatic and social isolation stress on mice social behavior

doi: 10.1016/j.ynstr.2025.100773

Figure Lengend Snippet: Effects of chronic stress on the protein expression levels of Fabp7 and Cxcl12 in the hippocampus and hypothalamus. (A) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hippocampus of the control and stress groups. Nuclei were counterstained with DAPI (blue). Scale bar: 100 μm, Zoom: 10 μm. (B, C) Quantification of the intensity and area of Fabp7 immunofluorescence in the hippocampus of the control and stress mice. (D) Representative confocal images showing the immunofluorescence staining of Fabp7 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (E, F) Quantification of the intensity and area of Fabp7 immunofluorescence in the hypothalamus of the control and stress groups. (G) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hippocampus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (H, I) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hippocampus of the control and stress groups. (J) Representative confocal images showing the immunofluorescence staining of Cxcl12 (green) in the hypothalamus of the control and stress groups. Scale bar: 100 μm, Zoom: 10 μm. (K, L) Quantification of the intensity and area of Cxcl12 immunofluorescence in the hypothalamus of the control and stress groups. All data are presented as mean ± SEM (n = 3–5 mice per group). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 stress vs control in adolescence or adolescence vs adulthood in the stress group (unpaired t -test).

Article Snippet: The sections were then blocked with a solution containing 2 % bovine serum albumin and 0.3 % Triton X-100 in PBS for 1 h. Primary antibodies against Fabp7 (mouse, 1:1000, NBP3-05531, Novus Biologicals, Littleton, CO, USA), Egr2 (rabbit, 1:2000, ab245228, Abcam, Cambridge, UK), and Cxcl12 (mouse, 1:100, MA5-23759, Invitrogen, Waltham, MA, USA) were applied overnight at 4 °C.

Techniques: Expressing, Immunofluorescence, Staining, Control