Journal: PeerJ

Article Title: Coagulation factor II receptor-like 1 as a prognostic and immuno-modulatory factor in head and neck squamous cell carcinoma

doi: 10.7717/peerj.20970

Figure Lengend Snippet: (A)The expression pattern of F2RL1 across 33 cancer types in the TCGA database. (B–D) Volcano plots showing the differential expression of molecules in the GSE9844 , GSE55548 , and GSE55547 GEO datasets, with circled positions representing F2RL1. (E) Upregulated expression of F2RL1 in HNSCC compared to normal tissues. (F) Upregulated expression of F2RL1 in paired tissue samples of HNSCC. (G) Validation of F2RL1 expression by RT-qPCR, with experimental groups including HNSCC tumor tissues and related cell lines FaDu and HN-6, and a control group consisting of normal human oral epithelial cells. (M) Western blot results for each group. (H–L) Immunohistochemical staining of F2RL1 in HNSCC from various sources in the HPA database, including adenocarcinoma (H, J), squamous cell carcinoma of oral tissue (I), lymph node (K), skeletal muscle (L).

Article Snippet: Immunohistochemistry information regarding F2RL1 was acquired from the Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Expressing, Quantitative Proteomics, Biomarker Discovery, Quantitative RT-PCR, Control, Western Blot, Immunohistochemical staining, Staining

Journal: PeerJ

Article Title: Coagulation factor II receptor-like 1 as a prognostic and immuno-modulatory factor in head and neck squamous cell carcinoma

doi: 10.7717/peerj.20970

Figure Lengend Snippet: (A–E) Comparison of F2RL1 levels in HNSCC patients with various clinical variables revealed significant differences in gender (A), race (B), histology grade (C), radiation therapy (D), and neck lymph node metastasis (E). (G) ROC analysis showed an AUC of 0.741. (H) ROC curve for the histology grade clinical variable (G1&G2 vs G3&G4) had an AUC of 0.617. (J, K) Kaplan–Meier curves for OS and DSS according to different F2RL1 expression levels. (I) Line plots illustrating the nanogram clinical prediction models for 1-year, 3-year, and 5-year survival of HNSCC patients. (F) Prognostic calibration correction curve.

Article Snippet: Immunohistochemistry information regarding F2RL1 was acquired from the Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Comparison, Expressing

Journal: PeerJ

Article Title: Coagulation factor II receptor-like 1 as a prognostic and immuno-modulatory factor in head and neck squamous cell carcinoma

doi: 10.7717/peerj.20970

Figure Lengend Snippet: (A) Venn diagram illustrating immune-related genes. (B) Heatmap displaying the co-expression of immune-related genes and F2RL1. (C) Protein-protein interaction analysis of immune-related genes. (D) Selection of 10 HUB genes utilizing the Cytoscape plugin CytoHubba. (E) Chord diagram showing the correlation between HUB genes. (F) Comparison of expression levels of HUB genes in HNSCC tissues and normal tissues.

Article Snippet: Immunohistochemistry information regarding F2RL1 was acquired from the Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Expressing, Selection, Comparison

Journal: PeerJ

Article Title: Coagulation factor II receptor-like 1 as a prognostic and immuno-modulatory factor in head and neck squamous cell carcinoma

doi: 10.7717/peerj.20970

Figure Lengend Snippet: (A) Heatmap displaying the top 15 genes positively and negatively correlated with F2RL1 expression in HNSCC. (B) GO and KEGG analysis performed on F2RL1-associated genes in HNSCC. (C, D) GSEA analysis conducted on F2RL1-associated genes in HNSCC. (C) Represents positive correlation, while (D) represents negative correlation. (E) GO and KEGG analysis conducted on F2RL1 with immune-related genes in HNSCC.

Article Snippet: Immunohistochemistry information regarding F2RL1 was acquired from the Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Expressing

Journal: PeerJ

Article Title: Coagulation factor II receptor-like 1 as a prognostic and immuno-modulatory factor in head and neck squamous cell carcinoma

doi: 10.7717/peerj.20970

Figure Lengend Snippet: (A) Comparison of F2RL1 expression levels and enrichment scores of 24 immune cell types. (B) Heatmap displaying the correlation between F2RL1-related 10 HUB genes and 24 immune cell types. (C) Lollipop chart exhibiting the correlation results between F2RL1 and immune infiltration. (D–G) Analysis of the correlation between F2RL1 and individual immune cells in HNSCC. (H–K) Impact of F2RL1 on survival curves with different immune cell infiltration patterns in HNSCC. (L–O) Immune checkpoints associated with F2RL1 in HNSCC.

Article Snippet: Immunohistochemistry information regarding F2RL1 was acquired from the Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Comparison, Expressing

Journal: PeerJ

Article Title: Coagulation factor II receptor-like 1 as a prognostic and immuno-modulatory factor in head and neck squamous cell carcinoma

doi: 10.7717/peerj.20970

Figure Lengend Snippet: (A) Heatmap depicting the methylation levels of the F2RL1 gene in HNSCC. (B) Genomic localization of the F2RL1 gene on the chromosome. (C) Common mutation sites and frequencies of the F2RL1 gene in HNSCC. (D–L) Kaplan–Meier curves illustrating the prognostic significance of individual CpG sites within the F2RL1 gene in HNSCC.

Article Snippet: Immunohistochemistry information regarding F2RL1 was acquired from the Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Methylation, Mutagenesis

Journal: PeerJ

Article Title: Coagulation factor II receptor-like 1 as a prognostic and immuno-modulatory factor in head and neck squamous cell carcinoma

doi: 10.7717/peerj.20970

Figure Lengend Snippet: (A) Heatmap illustrating the co-expression of F2RL1 with 20 m6A-related genes in HNSCC. (B–M) Scatter plots showing the co-expression of F2RL1 with m6A-related genes in HNSCC. Significantly different expressions were observed for IGF2BP2, VIRMA, FTO, ZC3H13, YTHDF3, HNRNPC, YTHDF1, YTHDF2, IGF2BP1, METTL3, RBM15B, and METTL14. (N–S) Kaplan–Meier survival curves depicting the prognostic significance of F2RL1 with 20 m6A-related genes in HNSCC. Among them, IGF2BP2, IGF2BP3, FTO, HNRNPC, IGF2BP1, and YTHDC2 exhibited prognostic value in HNSCC.

Article Snippet: Immunohistochemistry information regarding F2RL1 was acquired from the Human Protein Atlas (HPA) database ( http://www.proteinatlas.org/ ).

Techniques: Expressing

Journal: Future Science OA

Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

doi: 10.1080/20565623.2025.2535221

Figure Lengend Snippet: PAR2 expression level in lung cancer cells transfected either with pcDNA3-PAR2 or PAR2 shRNA. (A) PAR2 expression level after A549 cells were transfected with pcDNA3-PAR2; (B) PAR2 expression level after H1299 cells were transfected with pcDNA3-PAR2; (C) PAR2 expression level after A549 cells were transfected with PAR2 shRNA; (D) PAR2 expression level after H1299 cells were transfected with PAR2 shRNA.

Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

Techniques: Expressing, Transfection, shRNA

Journal: Future Science OA

Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

doi: 10.1080/20565623.2025.2535221

Figure Lengend Snippet: Overexpression PAR2 promoted growth of lung cancer cells with or without paclitaxel. (A–D) The growth of A549 cells with or without paclitaxel after transfection with pcDNA3-PAR2; (E–H) The growth of H1299 cells with or without paclitaxel after transfection with pcDNA3-PAR2.

Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

Techniques: Over Expression, Transfection

Journal: Future Science OA

Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

doi: 10.1080/20565623.2025.2535221

Figure Lengend Snippet: Knockdown PAR2 decreased growth of lung cancer cells with or without paclitaxel. (A–D) The growth of A549 cells with or without paclitaxel after transfection with PAR2 shRNA; (E–H) The growth of H1299 cells with or without paclitaxel after transfection with PAR2 shRNA.

Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

Techniques: Knockdown, Transfection, shRNA

Journal: Future Science OA

Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

doi: 10.1080/20565623.2025.2535221

Figure Lengend Snippet: PAR2 inhibited paclitaxel-associated apoptosis in lung cancer cells. (A) Caspase 3/7 activity in A549 cell transfected with pcDNA3-PAR2; (B) Caspase 3/7 activity in H1299 cell transfected with pcDNA3-PAR2; (C–F) Bcl-2 and BAX expression in A549 and H1299 cells.

Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

Techniques: Activity Assay, Transfection, Expressing

Journal: Future Science OA

Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

doi: 10.1080/20565623.2025.2535221

Figure Lengend Snippet: PAR2 played essential roles in invasion and migration of lung cancer cells. (A, B) up-regulation of PAR2 increased migration and invasion of A549 cells. (C, D) down-regulation of PAR2 decreaased migration and invasion of A549 cells. (E, F) up-regulation of PAR2 increased migration and invasion of H1299 cells. (G, H) down-regulation of PAR2 decreased migration and invasion of H1299 cells.

Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

Techniques: Migration

Journal: Future Science OA

Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

doi: 10.1080/20565623.2025.2535221

Figure Lengend Snippet: PAR2 altered PTEN/AKT protein expression in lung cancer cells. (A–C) Impact of up-regulation of PAR2 on expression of p-AKT, AKT and PTEN in A549 and H1299 cells. (D–F) Impact of down-regulation of PAR2 on expression of p-AKT, AKT and PTEN in A549 and H1299 cells.

Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

Techniques: Expressing

Journal: Future Science OA

Article Title: PAR2 regulates proliferation, migration of lung cancer and chemotherapy sensitivity by involving PTEN pathway

doi: 10.1080/20565623.2025.2535221

Figure Lengend Snippet: PAR2 expression in human lung cancer tissue. (A) PAR2 levels in different stage of lung cancer tissue and normal lung tissue. (B) Immunohistochemical studies for Ki-67 and PTEN on different levels of PAR2 in lung tissue.

Article Snippet: The primers were synthesized by Origene (USA) and the sequences were as follow: PAR2 forward: 5′-CTCCTCTCTGTCATCTGGTTCC-3′ and reverse 5′-TGCACACTGAGGCAGGTCATGA-3′.

Techniques: Expressing, Immunohistochemical staining