expression plasmid encoding rbfox1 (OriGene)
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OriGene
expression plasmid encoding rbfox1
Expression Plasmid Encoding Rbfox1, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+plasmids/RBFOX1+(NM_018723)+Human+Tagged+ORF+Clone/pm42904565-63-20-24
Average 94 stars, based on 1 article reviews
Expression Plasmid Encoding Rbfox1, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/expression+plasmids/RBFOX1+(NM_018723)+Human+Tagged+ORF+Clone/pm42904565-63-20-24
Average 94 stars, based on 1 article reviews
expression plasmid encoding rbfox1 - by Bioz Stars,
2026-11
94/100 stars
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Generated:Article Title: Potential Interaction of Pinocembrin with Drug Transporters and Hepatic Drug-Metabolizing Enzymes Article Snippet: The Chinese hamster ovary (CHO-K1) cells obtained from CLS (Deutschland, Germany) were cultured in complete Kaighn’s modification of Ham’s F-12 (with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin). .. The CHO-K1 cells overexpressing hOCT1, hOCT2, hMATE1, and hMATE2 were generated by the transfection of parent CHO-K1 cells with the Article Title: Autosomal recessive HOXA3 deficiency causes congenital athymia and laryngeal malformation. Article Snippet: The promoter of mouse Zfp703 (Origene, CAT#: MR213466) was cloned into the pGL4.10[luc2] firefly reporter vector (Promega). .. Transfection:Article Title: Potential Interaction of Pinocembrin with Drug Transporters and Hepatic Drug-Metabolizing Enzymes Article Snippet: The Chinese hamster ovary (CHO-K1) cells obtained from CLS (Deutschland, Germany) were cultured in complete Kaighn’s modification of Ham’s F-12 (with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin). .. The CHO-K1 cells overexpressing hOCT1, hOCT2, hMATE1, and hMATE2 were generated by the transfection of parent CHO-K1 cells with the Article Title: Potential Interaction of Pinocembrin with Drug Transporters and Hepatic Drug-Metabolizing Enzymes Article Snippet: Madin–Darby canine kidney (MDCK) cells obtained from AddexBio Technologies (San Diego, CA, USA) were maintained in minimum essential medium (MEM) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. .. To generate MDCK cells overexpressing hOAT1 (hOAT1-MDCK) and hOAT3 (OAT3-MDCK), the parent MDCK cells were transfected with the Article Title: Potential Interaction of Pinocembrin with Drug Transporters and Hepatic Drug-Metabolizing Enzymes Article Snippet: Madin–Darby canine kidney (MDCK) cells obtained from AddexBio Technologies (San Diego, CA, USA) were maintained in minimum essential medium (MEM) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. .. To generate MDCK cells overexpressing hOAT1 (hOAT1-MDCK) and hOAT3 (OAT3-MDCK), the parent MDCK cells were transfected with the Expressing:Article Title: Potential Interaction of Pinocembrin with Drug Transporters and Hepatic Drug-Metabolizing Enzymes Article Snippet: The Chinese hamster ovary (CHO-K1) cells obtained from CLS (Deutschland, Germany) were cultured in complete Kaighn’s modification of Ham’s F-12 (with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin). .. The CHO-K1 cells overexpressing hOCT1, hOCT2, hMATE1, and hMATE2 were generated by the transfection of parent CHO-K1 cells with the Article Title: A Novel Tetravalent CD95/Fas Fusion Protein With Superior CD95L/FasL Antagonism. Article Snippet: Antibodies were purchased from the following suppliers: SigmaAldrich, St. Louis, Missouri (anti- Flag M2, #F- 3165; anti- β- actin #A1978- 200; horseradish peroxidase [HRP] coupled anti- rabbit antibody, #7074), BD Biosciences, NJ (anti- PARP, #551025), Cell Signaling, MA (anti- Caspase- 9, #9502; anti- Caspase- 3 #8G10; anti- IκBα #L35A5; anti- P- IκBα #14D4), Enzo life sciences (anti- Caspase- 8 #ADI- AAM- 118- E) and Dako, Glostrup, Denmark ([HRP]- conjugated polyclonal rabbit anti- mouse antibody, #P0260). .. Article Title: Potential Interaction of Pinocembrin with Drug Transporters and Hepatic Drug-Metabolizing Enzymes Article Snippet: Madin–Darby canine kidney (MDCK) cells obtained from AddexBio Technologies (San Diego, CA, USA) were maintained in minimum essential medium (MEM) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. .. To generate MDCK cells overexpressing hOAT1 (hOAT1-MDCK) and hOAT3 (OAT3-MDCK), the parent MDCK cells were transfected with the Article Title: Potential Interaction of Pinocembrin with Drug Transporters and Hepatic Drug-Metabolizing Enzymes Article Snippet: Madin–Darby canine kidney (MDCK) cells obtained from AddexBio Technologies (San Diego, CA, USA) were maintained in minimum essential medium (MEM) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. .. To generate MDCK cells overexpressing hOAT1 (hOAT1-MDCK) and hOAT3 (OAT3-MDCK), the parent MDCK cells were transfected with the Article Title: Autosomal recessive HOXA3 deficiency causes congenital athymia and laryngeal malformation. Article Snippet: The promoter of mouse Zfp703 (Origene, CAT#: MR213466) was cloned into the pGL4.10[luc2] firefly reporter vector (Promega). .. Article Title: FVIII Trafficking Dynamics Across Subcellular Organelles Using CRISPR/Cas9 Specific Gene Knockouts. Article Snippet: Knockout cell lines were then transfected using Lipofectamine 2000 (Thermo Fisher #11668019, Langerwehe, Germany). .. Lipofectamine was mixed with 2 μg Article Title: Druggable genome CRISPRi screen in 3D hydrogels reveals regulators of cortactin-driven actin remodeling in invading glioblastoma cells Article Snippet: The coding sequences for the amino acids of ACP1 and AURKB were cloned onto this vector using Gibson Assembly (NEB Gibson Assembly Master Mix, E2611S). .. The protein sequences of ACP1 and AURKB were PCR amplified from Plasmid Preparation:Article Title: Potential Interaction of Pinocembrin with Drug Transporters and Hepatic Drug-Metabolizing Enzymes Article Snippet: The Chinese hamster ovary (CHO-K1) cells obtained from CLS (Deutschland, Germany) were cultured in complete Kaighn’s modification of Ham’s F-12 (with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin). .. The CHO-K1 cells overexpressing hOCT1, hOCT2, hMATE1, and hMATE2 were generated by the transfection of parent CHO-K1 cells with the Article Title: Potential Interaction of Pinocembrin with Drug Transporters and Hepatic Drug-Metabolizing Enzymes Article Snippet: Madin–Darby canine kidney (MDCK) cells obtained from AddexBio Technologies (San Diego, CA, USA) were maintained in minimum essential medium (MEM) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. .. To generate MDCK cells overexpressing hOAT1 (hOAT1-MDCK) and hOAT3 (OAT3-MDCK), the parent MDCK cells were transfected with the Article Title: Potential Interaction of Pinocembrin with Drug Transporters and Hepatic Drug-Metabolizing Enzymes Article Snippet: Madin–Darby canine kidney (MDCK) cells obtained from AddexBio Technologies (San Diego, CA, USA) were maintained in minimum essential medium (MEM) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. .. To generate MDCK cells overexpressing hOAT1 (hOAT1-MDCK) and hOAT3 (OAT3-MDCK), the parent MDCK cells were transfected with the other:Article Title: Highly conserved brain vascular receptor ALPL mediates transport of engineered AAV vectors across the blood-brain barrier. Article Snippet: 504 HEK293T-ALPL, HeLa-ALPL and MDCK-ALPL stable cell lines were generated with Chloramphenicol Acetyltransferase Assay:Article Title: Autosomal recessive HOXA3 deficiency causes congenital athymia and laryngeal malformation. Article Snippet: The promoter of mouse Zfp703 (Origene, CAT#: MR213466) was cloned into the pGL4.10[luc2] firefly reporter vector (Promega). .. Variant Assay:Article Title: Autosomal recessive HOXA3 deficiency causes congenital athymia and laryngeal malformation. Article Snippet: The promoter of mouse Zfp703 (Origene, CAT#: MR213466) was cloned into the pGL4.10[luc2] firefly reporter vector (Promega). .. Mutagenesis:Article Title: Autosomal recessive HOXA3 deficiency causes congenital athymia and laryngeal malformation. Article Snippet: The promoter of mouse Zfp703 (Origene, CAT#: MR213466) was cloned into the pGL4.10[luc2] firefly reporter vector (Promega). .. Polymerase Chain Reaction:Article Title: Druggable genome CRISPRi screen in 3D hydrogels reveals regulators of cortactin-driven actin remodeling in invading glioblastoma cells Article Snippet: The coding sequences for the amino acids of ACP1 and AURKB were cloned onto this vector using Gibson Assembly (NEB Gibson Assembly Master Mix, E2611S). .. The protein sequences of ACP1 and AURKB were PCR amplified from Amplification:Article Title: Druggable genome CRISPRi screen in 3D hydrogels reveals regulators of cortactin-driven actin remodeling in invading glioblastoma cells Article Snippet: The coding sequences for the amino acids of ACP1 and AURKB were cloned onto this vector using Gibson Assembly (NEB Gibson Assembly Master Mix, E2611S). .. The protein sequences of ACP1 and AURKB were PCR amplified from |
![( A ) Volcano plot showing dysregulated genes in lung adenocarcinoma tumors (TCGA-LUAD cohort) carrying driver mutations in NFE2L2 or KEAP1 [cutoffs: Log2(FC) = ±0.6 and P -adj ≤ 0.05]. ( B ) Gene Set Enrichment Analysis (GSEA) using Kyoto Encyclopedia of Genes and Genomes (KEGG) gene sets in the TCGA-LUAD cohort with mutations in NFE2L2 or KEAP1 . ( C ) mRNA expression of selected genes involved in formaldehyde (FA) detoxification in KEAP1-NFE2L2 mutated and nonmutated (WT) LUAD tumors. ( D ) mRNA expression of selected genes in normal lung and whole blood obtained from GTEX. ( E ) Single-cell RNA-seq data from mouse hematopoietic and progenitor cells (HPC) plotted with kleintools ( https://kleintools.hms.harvard.edu/tools/spring.html ) . ( F ) Representative images of Western blots of selected factors from (A) performed in different cell lines. ( G ) Cystine uptake assay performed in selected cell lines (mean ± SEM, n = 3). ( H ) FA tolerance of A549 cells lacking SLC7A11 (sg SLC7A11 ) complemented with a wild-type copy of SLC7A11 (pSLC7A11) grown in the presence of 100 μM BSO (mean ± SEM, n = 3 and two-way ANOVA, **** P < 0.0001). ( I ) FA tolerance for H1299 cells harboring empty vector (pEV) or pSLC7A11 in the presence of 100 μM BSO [mean ± SEM, n = 3 and two-way ANOVA (Tukey test between untreated (UT) and BSO at each point); for H1299 pEV: **** P < 0.0001 and *** P = 0.0002]. ( J ) Viability of A549 cells carrying sg SLC7A11 and sgNTC (nontargeting control) under cystine deprivation [one-way ANOVA (Tukey test)]. ( K ) Glutathione (GSH) levels of the indicated cell lines maintained during 48 hours under cystine deprivation and exposing to FA during the last 24 hours [one-way ANOVA (Tukey test), ** P = 0.0058]. ( L ) FA tolerance of A549 cells lacking ADH5 in the presence of BSO (100 μM) or ERA (5 μM) [mean ± SEM, n = 6 and two-way ANOVA (Tukey test between UT and BSO or ERA at each point for multiple comparison); * P = 0.0300 and **** P < 0.0001].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3651/pmc13693651/pmc13693651__sciadv.aea1431-f2.jpg)
