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Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; <t>EXP2:</t> NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).
Exp2, supplied by Nobilis Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exp2/pmc12722989-78-9-15?v=Nobilis+Therapeutics
Average 86 stars, based on 1 article reviews
exp2 - by Bioz Stars, 2026-08
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mouse anti exp2  (Proteintech)
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Proteintech mouse anti exp2
Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; <t>EXP2:</t> NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).
Mouse Anti Exp2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exp2/pmc12747541-341-28-47?v=Proteintech
Average 93 stars, based on 1 article reviews
mouse anti exp2 - by Bioz Stars, 2026-08
93/100 stars
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nonlinear curve fitting algorithm exp2  (MathWorks Inc)
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MathWorks Inc nonlinear curve fitting algorithm exp2
Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; <t>EXP2:</t> NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).
Nonlinear Curve Fitting Algorithm Exp2, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exp2/us12316352-296-9-14?v=MathWorks+Inc
Average 90 stars, based on 1 article reviews
nonlinear curve fitting algorithm exp2 - by Bioz Stars, 2026-08
90/100 stars
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non-linear curve fitting algorithm exp2  (MathWorks Inc)
90
MathWorks Inc non-linear curve fitting algorithm exp2
Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; <t>EXP2:</t> NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).
Non Linear Curve Fitting Algorithm Exp2, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exp2/us12316352-297-13-19?v=MathWorks+Inc
Average 90 stars, based on 1 article reviews
non-linear curve fitting algorithm exp2 - by Bioz Stars, 2026-08
90/100 stars
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exponential model exp2 in  (MathWorks Inc)
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MathWorks Inc exponential model exp2 in
Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; <t>EXP2:</t> NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).
Exponential Model Exp2 In, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exp2/pm40007345-100-2-5?v=MathWorks+Inc
Average 90 stars, based on 1 article reviews
exponential model exp2 in - by Bioz Stars, 2026-08
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mouse anti-exp2  (European Malaria Reagent)
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Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; <t>EXP2:</t> NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).
Mouse Anti Exp2, supplied by European Malaria Reagent, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exp2/pmc12011295-305-7-10?v=European+Malaria+Reagent
Average 90 stars, based on 1 article reviews
mouse anti-exp2 - by Bioz Stars, 2026-08
90/100 stars
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monoclonal antibody 7.7 (α-exp2)  (European Malaria Reagent)
90
European Malaria Reagent monoclonal antibody 7.7 (α-exp2)
Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; <t>EXP2:</t> NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).
Monoclonal Antibody 7.7 (α Exp2), supplied by European Malaria Reagent, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exp2/pm40198740-473-0-8?v=European+Malaria+Reagent
Average 90 stars, based on 1 article reviews
monoclonal antibody 7.7 (α-exp2) - by Bioz Stars, 2026-08
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rabbit anti-exp2 antibodies  (Gilson Inc)
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Gilson Inc rabbit anti-exp2 antibodies
Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; <t>EXP2:</t> NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).
Rabbit Anti Exp2 Antibodies, supplied by Gilson Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/exp2/pm40185976-302-53-62?v=Gilson+Inc
Average 90 stars, based on 1 article reviews
rabbit anti-exp2 antibodies - by Bioz Stars, 2026-08
90/100 stars
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Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; EXP2: NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).

Journal: Poultry Science

Article Title: Molecular characterization of the effects of essential oils on mRNA gene expression related to proinflammatory and antiviral pathways, virus load, and antibody production in chickens vaccinated against Newcastle disease virus, infectious bursal disease virus, and infectious bronchitis virus

doi: 10.1016/j.psj.2025.106178

Figure Lengend Snippet: Schematic representation of the experimental design of the study. Six hundred one-day-old broiler chickens Ross AP were distributed in four experiments (EXP) (150 chickens for each Experiment) [EXP1: no vaccine; EXP2: NDV vaccine virus (NOBILIS ND clone 30); EXP3: IBDV vaccine virus (Hipragumboro strain Winterfield clone CH/80), and EXP4: IBV vaccine virus (Hipraviar H120)]. The 150 chickens were randomly distributed in the 15 pens (ten chickens per pen), considering each pen an experimental unit. In each EXP, the following treatments were applied: T1 (Control): distilled water as a spray; T2 (EO blend): EO mixture (1,500 ppm in spray); T3 (EO Lippia alba ): Lippia alba essential oil (1,500 ppm in spray).

Article Snippet: The experimental design was as follows: EXP1, unvaccinated control; EXP2, Newcastle disease virus (NDV) vaccine (Nobilis ND LaSota Clone 30, 6.0 log10 EID50 dose); EXP3, infectious bursal disease virus (IBDV) vaccine (Hipragumboro, Winterfield CH/80 clone, 3.5 log10 TCID50 dose); and EXP4, infectious bronchitis virus (IBV) vaccine (Hipraviar Massachusetts H120, 6.5 log10 EID50 dose).

Techniques: Virus, Control

Relative mRNA expression (2⁻ΔΔCt) of innate immune-related genes in chickens from EX 2–4 vaccinated with different live viral vaccines. (A) EXP2 (NDV, trachea); (B) EXP3 (IBDV, bursa of Fabricius); (C) EXP4 (IBV, trachea). Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). Bars connected by * or ** denote significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each gene, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. TLR3, Toll-like receptor 3; TLR7, Toll-like receptor 7; MDA5, melanoma differentiation-associated gene 5; NF-κB, nuclear factor κB; IL-1β, interleukin 1β; IFN-β, interferon β; OAS, 2′−5′-oligoadenylate synthetase; PKR, protein kinase R; EO, essential oil.

Journal: Poultry Science

Article Title: Molecular characterization of the effects of essential oils on mRNA gene expression related to proinflammatory and antiviral pathways, virus load, and antibody production in chickens vaccinated against Newcastle disease virus, infectious bursal disease virus, and infectious bronchitis virus

doi: 10.1016/j.psj.2025.106178

Figure Lengend Snippet: Relative mRNA expression (2⁻ΔΔCt) of innate immune-related genes in chickens from EX 2–4 vaccinated with different live viral vaccines. (A) EXP2 (NDV, trachea); (B) EXP3 (IBDV, bursa of Fabricius); (C) EXP4 (IBV, trachea). Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). Bars connected by * or ** denote significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each gene, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. TLR3, Toll-like receptor 3; TLR7, Toll-like receptor 7; MDA5, melanoma differentiation-associated gene 5; NF-κB, nuclear factor κB; IL-1β, interleukin 1β; IFN-β, interferon β; OAS, 2′−5′-oligoadenylate synthetase; PKR, protein kinase R; EO, essential oil.

Article Snippet: The experimental design was as follows: EXP1, unvaccinated control; EXP2, Newcastle disease virus (NDV) vaccine (Nobilis ND LaSota Clone 30, 6.0 log10 EID50 dose); EXP3, infectious bursal disease virus (IBDV) vaccine (Hipragumboro, Winterfield CH/80 clone, 3.5 log10 TCID50 dose); and EXP4, infectious bronchitis virus (IBV) vaccine (Hipraviar Massachusetts H120, 6.5 log10 EID50 dose).

Techniques: Expressing, Vaccines, Comparison

Antibody titers against NDV, IBDV, and IBV in chickens from the four experimental groups. (A) EXP1 (non-vaccinated birds), (B) EXP2–4, birds vaccinated with NDV, IBDV, or IBV, respectively. Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). NDV antibodies were measured by hemagglutination inhibition (HI) and expressed as log₂ HI titers. IBDV and IBV antibodies were quantified by ELISA and expressed as sample-to-positive (S/P) ratios. Bars connected by * or ** indicate significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each virus, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. NDV, Newcastle disease virus; IBDV, infectious bursal disease virus; IBV, infectious bronchitis virus; EO, essential oil.

Journal: Poultry Science

Article Title: Molecular characterization of the effects of essential oils on mRNA gene expression related to proinflammatory and antiviral pathways, virus load, and antibody production in chickens vaccinated against Newcastle disease virus, infectious bursal disease virus, and infectious bronchitis virus

doi: 10.1016/j.psj.2025.106178

Figure Lengend Snippet: Antibody titers against NDV, IBDV, and IBV in chickens from the four experimental groups. (A) EXP1 (non-vaccinated birds), (B) EXP2–4, birds vaccinated with NDV, IBDV, or IBV, respectively. Samples were collected at 21 (7 dpv) and 28 days of age (14 dpv). NDV antibodies were measured by hemagglutination inhibition (HI) and expressed as log₂ HI titers. IBDV and IBV antibodies were quantified by ELISA and expressed as sample-to-positive (S/P) ratios. Bars connected by * or ** indicate significant differences ( p < 0.05 and p < 0.001, respectively) among treatments within each virus, based on one-way ANOVA (α = 0.05) followed by Tukey’s HSD multiple comparison test. NDV, Newcastle disease virus; IBDV, infectious bursal disease virus; IBV, infectious bronchitis virus; EO, essential oil.

Article Snippet: The experimental design was as follows: EXP1, unvaccinated control; EXP2, Newcastle disease virus (NDV) vaccine (Nobilis ND LaSota Clone 30, 6.0 log10 EID50 dose); EXP3, infectious bursal disease virus (IBDV) vaccine (Hipragumboro, Winterfield CH/80 clone, 3.5 log10 TCID50 dose); and EXP4, infectious bronchitis virus (IBV) vaccine (Hipraviar Massachusetts H120, 6.5 log10 EID50 dose).

Techniques: HI Assay, Enzyme-linked Immunosorbent Assay, Virus, Comparison