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Hydroxysafflor yellow A (HSYA) enhanced vascular plaque stability and alleviated aortic endothelial permeability in ApoE −/− mice. (a, b) Representative immunohistochemical images and quantification of VCAM‐1 expression in the aortic root. Scale bar: 200 μm. (c, d) Representative images of TUNEL <t>staining</t> and quantitative analysis. Scale bar: 200 μm. (e, f) <t>Evans</t> <t>blue</t> staining of the mid‐aorta and determination of Evans blue content. Scale bar: 200 μm. Data were presented as the mean ± SEM ( n = 3). ### p < .001 versus Ctrl group; *** p < .001 versus Model group.
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Hydroxysafflor yellow A (HSYA) enhanced vascular plaque stability and alleviated aortic endothelial permeability in ApoE −/− mice. (a, b) Representative immunohistochemical images and quantification of VCAM‐1 expression in the aortic root. Scale bar: 200 μm. (c, d) Representative images of TUNEL <t>staining</t> and quantitative analysis. Scale bar: 200 μm. (e, f) <t>Evans</t> <t>blue</t> staining of the mid‐aorta and determination of Evans blue content. Scale bar: 200 μm. Data were presented as the mean ± SEM ( n = 3). ### p < .001 versus Ctrl group; *** p < .001 versus Model group.
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Hydroxysafflor yellow A (HSYA) enhanced vascular plaque stability and alleviated aortic endothelial permeability in ApoE −/− mice. (a, b) Representative immunohistochemical images and quantification of VCAM‐1 expression in the aortic root. Scale bar: 200 μm. (c, d) Representative images of TUNEL <t>staining</t> and quantitative analysis. Scale bar: 200 μm. (e, f) <t>Evans</t> <t>blue</t> staining of the mid‐aorta and determination of Evans blue content. Scale bar: 200 μm. Data were presented as the mean ± SEM ( n = 3). ### p < .001 versus Ctrl group; *** p < .001 versus Model group.
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Hydroxysafflor yellow A (HSYA) enhanced vascular plaque stability and alleviated aortic endothelial permeability in ApoE −/− mice. (a, b) Representative immunohistochemical images and quantification of VCAM‐1 expression in the aortic root. Scale bar: 200 μm. (c, d) Representative images of TUNEL <t>staining</t> and quantitative analysis. Scale bar: 200 μm. (e, f) <t>Evans</t> <t>blue</t> staining of the mid‐aorta and determination of Evans blue content. Scale bar: 200 μm. Data were presented as the mean ± SEM ( n = 3). ### p < .001 versus Ctrl group; *** p < .001 versus Model group.
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Hydroxysafflor yellow A (HSYA) enhanced vascular plaque stability and alleviated aortic endothelial permeability in ApoE −/− mice. (a, b) Representative immunohistochemical images and quantification of VCAM‐1 expression in the aortic root. Scale bar: 200 μm. (c, d) Representative images of TUNEL <t>staining</t> and quantitative analysis. Scale bar: 200 μm. (e, f) <t>Evans</t> <t>blue</t> staining of the mid‐aorta and determination of Evans blue content. Scale bar: 200 μm. Data were presented as the mean ± SEM ( n = 3). ### p < .001 versus Ctrl group; *** p < .001 versus Model group.
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Hydroxysafflor yellow A (HSYA) enhanced vascular plaque stability and alleviated aortic endothelial permeability in ApoE −/− mice. (a, b) Representative immunohistochemical images and quantification of VCAM‐1 expression in the aortic root. Scale bar: 200 μm. (c, d) Representative images of TUNEL <t>staining</t> and quantitative analysis. Scale bar: 200 μm. (e, f) <t>Evans</t> <t>blue</t> staining of the mid‐aorta and determination of Evans blue content. Scale bar: 200 μm. Data were presented as the mean ± SEM ( n = 3). ### p < .001 versus Ctrl group; *** p < .001 versus Model group.
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Hydroxysafflor yellow A (HSYA) enhanced vascular plaque stability and alleviated aortic endothelial permeability in ApoE −/− mice. (a, b) Representative immunohistochemical images and quantification of VCAM‐1 expression in the aortic root. Scale bar: 200 μm. (c, d) Representative images of TUNEL staining and quantitative analysis. Scale bar: 200 μm. (e, f) Evans blue staining of the mid‐aorta and determination of Evans blue content. Scale bar: 200 μm. Data were presented as the mean ± SEM ( n = 3). ### p < .001 versus Ctrl group; *** p < .001 versus Model group.

Journal: Food Science & Nutrition

Article Title: Hydroxysafflor yellow A, a natural food pigment, ameliorates atherosclerosis in ApoE −/− mice by inhibiting the SphK1 / S1P / S1PR3 pathway

doi: 10.1002/fsn3.4466

Figure Lengend Snippet: Hydroxysafflor yellow A (HSYA) enhanced vascular plaque stability and alleviated aortic endothelial permeability in ApoE −/− mice. (a, b) Representative immunohistochemical images and quantification of VCAM‐1 expression in the aortic root. Scale bar: 200 μm. (c, d) Representative images of TUNEL staining and quantitative analysis. Scale bar: 200 μm. (e, f) Evans blue staining of the mid‐aorta and determination of Evans blue content. Scale bar: 200 μm. Data were presented as the mean ± SEM ( n = 3). ### p < .001 versus Ctrl group; *** p < .001 versus Model group.

Article Snippet: One hour before execution, mice were injected with 0.5% 2 mL/kg Evans blue staining solution (Macklin, Shanghai, China) in the tail vein.

Techniques: Permeability, Immunohistochemical staining, Expressing, TUNEL Assay, Staining