Journal: Frontiers in Cell and Developmental Biology

Article Title: Sirt1 coordinates the mitochondrial UPR and myocellular proteostasis to preserve muscle integrity during muscle atrophy in zebrafish

doi: 10.3389/fcell.2026.1761278

Figure Lengend Snippet: Inhibition of the mFAO sensitizes muscle to atrophic stress. (a) qRT-PCR analysis of zebrafish larvae treated with DEXA(S) and/or etomoxir (ETO) showing the expression of the atrophy genes fbxo32 and trim63a (DEXA(S) indicates a short-course DEXA treatment. * p < 0.05, t-test, two-tailed, error bar indicates s.d.). (b) Representative fluorescent images of LysoView-stained zebrafish larvae treated with DEXA(S) and/or ETO. Histogram showing quantification of fluorescent intensity (white rectangle indicates the area chosen for the fluorescence quantification. Scale bar represents 1 mm. * p < 0.05, one-way ANOVA and Tukey’s multiple comparison test; error bar indicates s.d.). (c) Representative confocal microscopy images of Alexa488-phalloidin-stained zebrafish larvae treated with DEXA(S) and/or ETO with or without the hormesis treatment (arrows indicate examples of detached muscle fibers). Boxplot shows quantification of larvae with 0, 1–2, 3–15, or >15 detached muscle fibers (* p < 0.05, Chi-square test. Scale bar represents 50 µM).

Article Snippet: To block Cpt1 activity, larval zebrafish at 4 dpf were treated with 10 μM etomoxir (ETO) (Selleckchem) with or without DEXA for 18 h–20 h. To suppress autophagy, 5 mM chloroquine (Sigma) was added to the larval zebrafish medium for 1-h incubation.

Techniques: Inhibition, Quantitative RT-PCR, Expressing, Two Tailed Test, Staining, Fluorescence, Comparison, Confocal Microscopy