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(A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), <t>IRS1,</t> <t>EGFR,</t> ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, <t>ERα,</t> Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.
Erα, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/er%CE%B1/Estrogen+Receptor+alpha+Antibody/bio_rxiv__64898__2026__09__22__753596-79-32-44
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erα - by Bioz Stars, 2026-10
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mouse anti erα  (Santa Cruz Biotechnology)
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(A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), <t>IRS1,</t> <t>EGFR,</t> ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, <t>ERα,</t> Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.
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mouse anti erα - by Bioz Stars, 2026-10
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p erα  (Wuling Biotechnology Co Ltd)
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(A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), <t>IRS1,</t> <t>EGFR,</t> ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, <t>ERα,</t> Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.
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(A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), <t>IRS1,</t> <t>EGFR,</t> ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, <t>ERα,</t> Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.
Goat Anti Human Cystatin B Stefin B, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti human cystatin b stefin b - by Bioz Stars, 2026-10
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erα  (Galectin Therapeutics)
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(A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), <t>IRS1,</t> <t>EGFR,</t> ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, <t>ERα,</t> Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.
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(A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), <t>IRS1,</t> <t>EGFR,</t> ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, <t>ERα,</t> Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.
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er alpha  (ABclonal Biotechnology)
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(A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), <t>IRS1,</t> <t>EGFR,</t> ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, <t>ERα,</t> Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.
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(A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), <t>IRS1,</t> <t>EGFR,</t> ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, <t>ERα,</t> Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.
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Image Search Results


(A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), IRS1, EGFR, ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, ERα, Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.

Journal: bioRxiv

Article Title: Transcriptomic Analysis of the Newborn Mammary Gland Identifies Prenatal Ethanol-Induced Remodeling of Insulin Signaling and Enhanced Proliferative Programming

doi: 10.64898/2026.09.22.753596

Figure Lengend Snippet: (A) Relative mRNA expression of IGFBP3 and IGFBP5 in control, PAE-L, and PAE-M newborn mammary tissues determined by quantitative RT-PCR. (B) Representative Western blots of selected insulin/IGF- and growth-factor-associated signaling proteins, including p-IRS1(T895), p-IRS1(S612), IRS1, EGFR, ErbB3, IGF1R, p-Akt1(S473), Akt1, p-ERK1/2, ERK2, ERα, Sox2, and IGFBP5. (C) Densitometric analysis of Western blot data. Phosphorylated proteins were normalized to their corresponding total proteins, and total protein levels were normalized to β-Actin, as appropriate.

Article Snippet: Proteins examined included IRS1(#3407), phospho-IRS1 (Ser612) (#3203), phospho-IRS1 (Tyr895) (#3070), phospho-Akt1 (Ser473) (#4060), ErbB3 (#12708), phospho-ERK1/2 (Thr202/Tyr204) (#9101) were purchased from Cell signaling Technology, EGFR (sc-03), IGF1R (sc-712), Akt1 (sc-5298), Erk2 (sc-1647), ERα (sc-8002), Sox2 (sc-398254), IGFBP5 (sc-515116) and β-Actin (sc-47778) were purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot, Analysis