Journal: Brazilian Journal of Medical and Biological Research

Article Title: Knockout of Mucin 1 inhibits the proliferation, migration, and invasion of human MDA-MB-231 cells by blocking autophagy flow

doi: 10.1590/1414-431X2026e15075

Figure Lengend Snippet: Mucin 1 (MUC1) expression in human breast cancer. A , MUC1 expression levels in breast cancer (UALCAN website). B , MUC1 expression levels in different subtypes of breast cancer (UALCAN website). C , MUC1 protein expression in breast cancer (Human Protein Atlas database). D , MUC1 expression in human normal mammary epithelial cells MCF10A, TNBC cells (MDA-MB-231), and TNBC tissues (scale bar=100 μm). Data are reported as means±SE. **P<0.01, ***P<0.001, ****P<0.0001; two-tailed unpaired Student's t -test. BRCA: breast cancer gene; TCGA: The Cancer Genome Atlas; TNBC: triple-negative breast cancer.

Article Snippet: The human triple-negative breast cancer cell line MDA-MB-231 and the normal human breast epithelial cells MCF10A were obtained from the Procell Biotechnology Company (China).

Techniques: Expressing, Two Tailed Test

Journal: Frontiers in Pharmacology

Article Title: LITAF suppresses breast cancer and paclitaxel resistance by ubiquitinating and degrading PCMT1 to inhibit COX-2-dependent arachidonic acid metabolism

doi: 10.3389/fphar.2026.1706420

Figure Lengend Snippet: PCMT1 is involved in PTX resistance of BC cells, a process potentially involving COX-2-mediated AA metabolism. (A) RT-qPCR analysis of PCMT1 mRNA levels in tumor tissues and paired adjacent normal tissues from 30 BC patients treated with PTX. (B) Immunoblotting analysis of PCMT1 and COX-2 protein levels in tumor tissues and paired adjacent normal tissues from PTX-treated BC patients (n = 10; 5 PTX-sensitive and 5 PTX-resistant). (C) Correlation between PCMT1 and COX-2 expression was assessed using the Pearson correlation coefficient. (D) RT-qPCR analysis of PCMT1 mRNA levels in MCF10A, BC cells (MDA-MB-231, MCF-7), and PTX-resistant cells (MDA-MB-231/PTX, MCF-7/PTX) (n = 3). (E) Immunoblotting analysis of PCMT1 and COX-2 protein levels in BC cells and PTX-resistant cells (n = 3). (F) IC50 values of BC cells and PTX-resistant cells assessed using the CCK-8 assay (n = 3). Values are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Human normal mammary epithelial cells MCF10A (ATCC, USA) were cultured in DMEM/F12 (Procell) supplemented with HS (5%), EGF (20 ng/mL), hydrocortisone (0.5 μg/mL), insulin (0.5 μg/mL), NEAA (1%), and P/S (1%).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, CCK-8 Assay

Journal: Journal of Medicinal Chemistry

Article Title: Exploring Gallium(III) Complexes as Emerging Therapeutic Candidates for Breast Cancer

doi: 10.1021/acs.jmedchem.5c03480

Figure Lengend Snippet: (A) Dose–response curves of Ga3 and Ga6 compared to cisplatin in nontransformed cell lines HaCaT and MCF10A. (B) Selectivity Index values for Ga3 , Ga6 and cisplatin. Data represent the mean ± SD from three independent experiments performed in triplicate.

Article Snippet: The cell lines MCF7, SKBR3, BT549, and MDA-MB-231, the immortalized nontransformed keratinocyte cell line HaCaT, and the human mammary epithelial cell line MCF10A were acquired from ATCC.

Techniques:

Journal: Journal of Medicinal Chemistry

Article Title: Exploring Gallium(III) Complexes as Emerging Therapeutic Candidates for Breast Cancer

doi: 10.1021/acs.jmedchem.5c03480

Figure Lengend Snippet: Matrigel assay. (A) 3D structures of MCF7, MDA-MB-231 and MCF10A seeded in Matrigel matrix and treated with compound at indicated doses for 72-h. Scale bar = 250 μm. (B) The diameter of 3D structures was quantified using ImageJ. Data are the average ± SEM of three independent experiments. The values for the statistical analyses are * p ≤ 0.05; *** p ≤ 0.001.

Article Snippet: The cell lines MCF7, SKBR3, BT549, and MDA-MB-231, the immortalized nontransformed keratinocyte cell line HaCaT, and the human mammary epithelial cell line MCF10A were acquired from ATCC.

Techniques: Matrigel Assay