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Anti-PD-1 therapy improves the histopathology of lung and tumor tissue but induces myocarditis in wild-type mice. (A) Representative photographs of the lungs containing the tumors. HE staining was performed to examine the histopathology of (B) tumor (black arrow, nucleus; red arrow, inflammatory cell infiltration), (C) lung (arrow, interstitial congestion) and (D) heart tissue (black arrow, intercellular space; red arrow, inflammatory cell infiltration). (E) Masson's trichrome staining was performed to examine the histopathology of heart tissue. Arrow, collagen fiber. Scale bar, 50 µm. * P<0.05 vs. control, ### P<0.001 vs. LLC. HE, hematoxylin-eosin; LLC, Lewis lung carcinoma.

Journal: Biomedical Reports

Article Title: Preliminary exploration of the role of CD8 + T cells in anti-PD-1 antibody-induced myocarditis in C57BL/6 mice with Lewis lung carcinoma

doi: 10.3892/br.2026.2129

Figure Lengend Snippet: Anti-PD-1 therapy improves the histopathology of lung and tumor tissue but induces myocarditis in wild-type mice. (A) Representative photographs of the lungs containing the tumors. HE staining was performed to examine the histopathology of (B) tumor (black arrow, nucleus; red arrow, inflammatory cell infiltration), (C) lung (arrow, interstitial congestion) and (D) heart tissue (black arrow, intercellular space; red arrow, inflammatory cell infiltration). (E) Masson's trichrome staining was performed to examine the histopathology of heart tissue. Arrow, collagen fiber. Scale bar, 50 µm. * P<0.05 vs. control, ### P<0.001 vs. LLC. HE, hematoxylin-eosin; LLC, Lewis lung carcinoma.

Article Snippet: Masson's trichrome (cat. no. C0189S) and hematoxylin & eosin (HE) staining kit (cat. no. C0105S) were purchased from Beyotime Institute of Biotechnology.

Techniques: Histopathology, Staining, Control

Effects of anti-PD-1 therapy on the histopathology of tumor, lung and heart tissue in CD8 -/- mice. (A) Representative photographs of the lungs containing the tumors. HE staining was performed to examine the histopathology of (B) tumor (black arrow, nucleus; red arrow, inflammatory cell infiltration), (C) lung (arrow, interstitial congestion) and (D) heart tissue (black arrow, intercellular space; red arrow, inflammatory cell infiltration). (E) Masson's trichrome staining was performed to examine the histopathology of heart tissue. Arrow, collagen fiber. Scale bar, 50 µm. *** P<0.001. HE, hematoxylin-eosin; LLC, Lewis lung carcinoma; ns, not significant.

Journal: Biomedical Reports

Article Title: Preliminary exploration of the role of CD8 + T cells in anti-PD-1 antibody-induced myocarditis in C57BL/6 mice with Lewis lung carcinoma

doi: 10.3892/br.2026.2129

Figure Lengend Snippet: Effects of anti-PD-1 therapy on the histopathology of tumor, lung and heart tissue in CD8 -/- mice. (A) Representative photographs of the lungs containing the tumors. HE staining was performed to examine the histopathology of (B) tumor (black arrow, nucleus; red arrow, inflammatory cell infiltration), (C) lung (arrow, interstitial congestion) and (D) heart tissue (black arrow, intercellular space; red arrow, inflammatory cell infiltration). (E) Masson's trichrome staining was performed to examine the histopathology of heart tissue. Arrow, collagen fiber. Scale bar, 50 µm. *** P<0.001. HE, hematoxylin-eosin; LLC, Lewis lung carcinoma; ns, not significant.

Article Snippet: Masson's trichrome (cat. no. C0189S) and hematoxylin & eosin (HE) staining kit (cat. no. C0105S) were purchased from Beyotime Institute of Biotechnology.

Techniques: Histopathology, Staining