Journal: iScience

Article Title: The transcription factor IRF4 determines the anti-tumor immunity of CD8 + T cells

doi: 10.1016/j.isci.2023.108087

Figure Lengend Snippet: Key resources table

Article Snippet: The retroviral particles were generated by transfecting plat-E cells with the IRF4-GFP vector or the empty GFP-control vector, following the manufacturer's instructions (Cell Biolabs).

Techniques: Virus, Plasmid Preparation, Recombinant, Staining, Software

Journal: BMC Complementary Medicine and Therapies

Article Title: Chrysoeriol suppresses hyperproliferation of rheumatoid arthritis fibroblast-like synoviocytes and inhibits JAK2/STAT3 signaling

doi: 10.1186/s12906-022-03553-w

Figure Lengend Snippet: Over-activation of STAT3 attenuated the inhibitory effects of CSR on RA-FLS hyperproliferation. RA-FLS were transduced with Ad-STAT3C (RA-FLS STAT3C ) or Ad-Empty (RA-FLS Empty vector ) plasmid. ( a ) Green fluorescent protein (GFP) expression and the protein levels of phospho-STAT3 (Tyr 705), STAT3 and Flag in RA-FLS Empty vector and RA-FLS STAT3C . Cells expressing GFP displayed green fluorescence. Representative green fluorescent and bright-field microscopy images of RA-FLS Empty vector and RA-FLS STAT3C are shown in the left panel (Scale bar: 10 μm). Representative immunoblotting results are shown in the right panel. Each membrane was cut based on molecular weights of proteins to be examined before hybridisation with primary antibodies. Original images of all blots are presented in Additional file . ( b ) Over-activation of STAT3 attenuated the inhibitory effect of CSR on RA-FLS hyperproliferation. Transduced RA-FLS were incubated with indicated concentrations of CSR for 1 h and then stimulated with IL-6/sIL-6R (100 ng/ml each) for 48 h. Cell viability was assessed using CCK-8 assays. Data are expressed as mean ± SD of 3 independent experiments. Differences of relative cell viabilities between CSR-treated RA-FLS Empty vector and CSR-treated RA-FLS STAT3C were calculated. * P < 0.05, ** P < 0.01

Article Snippet: Adenovirus expressing green fluorescent protein (GFP)-flag-tagged STAT3C (Ad-STAT3C) and control adenovirus expressing GFP (Ad-Empty vector) were purchased from Vigene Biosciences (Shandong, China).

Techniques: Activation Assay, Transduction, Plasmid Preparation, Expressing, Fluorescence, Microscopy, Western Blot, Hybridization, Incubation, CCK-8 Assay

Journal: Journal of Ginseng Research

Article Title: Ginsenoside Rg3 in combination with artesunate overcomes sorafenib resistance in hepatoma cell and mouse models

doi: 10.1016/j.jgr.2021.07.002

Figure Lengend Snippet: Over-activation of STAT3 diminishes the inhibitory effects of Rg3-plus-ART on cell viability in HepG2-SR cells. HepG2-SR cells were transduced with the Ad-Empty vector (HepG2-SR Empty vector ) or the Ad-STAT3C plasmid (HepG2-SR STAT3C ). (A) GFP expression and the protein levels of STAT3, phospho-STAT3 (Tyr 705) and Flag in HepG2-SR Empty vector and HepG2-SR STAT3C cells. Representative green fluorescent and bright-field microscopy images of HepG2-SR Empty vector and HepG2-SR STAT3C (the left panel, scale bar, 10 μm), and representative immunoblotting results (the right panel) are shown. (B) Over-activation of STAT3 diminishes the anti-proliferative effects of Rg3-plus-ART in HepG2-SR cells. Differences of relative cell viabilities between Rg3-plus-ART-treated HepG2-SR Empty vector cells and Rg3-plus-ART-treated HepG2-SR STAT3C cells were calculated (n = 3). ∗ P < 0.05, ∗∗ P < 0.01.

Article Snippet: Adenovirus expressing GFP (green fluorescent protein)-Flag-tagged STAT3C (Ad-STAT3C) and control adenovirus expressing GFP (Ad-Empty vector) were purchased from Vigene Biosciences (Shandong, China).

Techniques: Activation Assay, Transduction, Plasmid Preparation, Expressing, Microscopy, Western Blot