Journal: Journal of Hepatocellular Carcinoma

Article Title: CT Radiomics Combined with Metabolic-Biomarkers Enables Early Recurrence Prediction in Hepatocellular Carcinoma

doi: 10.2147/JHC.S547186

Figure Lengend Snippet: Differential expression and prognostic value of EEF1E1. ( A ) Differential expression of EEF1E1 in TCGA-LIHC cohort, ( B ) OEP000321 cohort and ( C ) GSE14520 cohort. ( D ) Risk accumulation curve of EEF1E1 mRNA expression for early recurrence in TCGA-LIHC and ( E ) OEP000321 cohort. ( F ) Correlation between EEF1E1 mRNA expression with Rad-scores. ( G ) IHC stains image of EEF1E1 protein levels in HCC and para cancer tissues. (**** p < 0.0001).

Article Snippet: The main steps were as follows: rabbit anti-human EEF1E1 (Proteintech Cat No: 10805-1-AP, 1:200 dilution) was incubated at 4°C overnight, followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibody (ZSGB-BIO, ZB-2301) for one h at 37°C, and staining with 3,3-diaminobenzidine tetrahydrochloride (DAB).

Techniques: Quantitative Proteomics, Expressing

Journal: Journal of Hepatocellular Carcinoma

Article Title: CT Radiomics Combined with Metabolic-Biomarkers Enables Early Recurrence Prediction in Hepatocellular Carcinoma

doi: 10.2147/JHC.S547186

Figure Lengend Snippet: Relation between EEF1E1 protein levels and HCC recurrence status. ( A ) IHC stains image of EEF1E1 protein levels in different recurrence risk of HCC tissues. ( B ) Statistical analysis was performed using the Chi-square test to compare the relative levels of EEF1E1 negative (EEF1E1 − ) or positive (EEF1E1 + ) between HCC with recurrence and HCC without recurrence ( p = 0.010). ( C ) Boxplot of IHC scores of EEF1E1 between non-recurrence and recurrence patients. ( D ) Risk accumulation curve of EEF1E1 protein level for early recurrence in IHC validation cohort. ( E ) Correlation between Rad-scores and EEF1E1 IHC scores.

Article Snippet: The main steps were as follows: rabbit anti-human EEF1E1 (Proteintech Cat No: 10805-1-AP, 1:200 dilution) was incubated at 4°C overnight, followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibody (ZSGB-BIO, ZB-2301) for one h at 37°C, and staining with 3,3-diaminobenzidine tetrahydrochloride (DAB).

Techniques: Biomarker Discovery

Journal: Cell reports

Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome

doi: 10.1016/j.celrep.2021.109806

Figure Lengend Snippet: Existing studies frame rocaglates as pure eIF4A/translation inhibitors. This study conceptually redefines rocaglates as translation remodelers that reprogram both the translation machinery, e.g., increasing eEF1ε1 translational activity, as well as the translatome, including the upregulation of numerous proteins, e.g., GEF-H1, that mediate rocaglate cytotoxicity in cancer cells. As a proof of concept, GEF-H1 activates RHOA, leading to increased JNK phosphorylation and apoptotic signaling resulting in reduced cell viability.

Article Snippet: eEF1ε1 , Proteintech , Cat#10805-1-AP; RRID:AB_2097140.

Techniques: Activity Assay, Phospho-proteomics

Journal: Cell reports

Article Title: Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome

doi: 10.1016/j.celrep.2021.109806

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: eEF1ε1 , Proteintech , Cat#10805-1-AP; RRID:AB_2097140.

Techniques: Plasmid Preparation, Derivative Assay, Recombinant, Protease Inhibitor, cDNA Synthesis, SYBR Green Assay, Mass Spectrometry, Construct, Software

Journal: Frontiers in Oncology

Article Title: A Novel HCC Prognosis Predictor EEF1E1 Is Related to Immune Infiltration and May Be Involved in EEF1E1/ATM/p53 Signaling

doi: 10.3389/fonc.2021.700972

Figure Lengend Snippet: The expression of EEF1E1 in HCC. (A) Comparing the expression of EEF1E1 in cancer and normal. (B) Comparing the expression of EEF1E1 in cancer and paracancerous. (C) The expression of EEF1E1 in HCC from Human protein Atlas.

Article Snippet: The immunohistochemistry of EEF1E1 expression in HCC was downloaded from Human Protein Atlas ( ) ( http://www.proteinatlas.org ).

Techniques: Expressing