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Effects of analyzed extracts on the area of cerebral thrombosis of zebrafish larvae. (A) Area of cerebral thrombosis and (B) representative images of the zebrafish larvae (5 days post-fertilization). Area inside the dashed lines indicates the observed area. Scale bar, 50 µm. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml <t>edaravone;</t> Pon, 3 µg/ml ponatinib.
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edaravone dexborneol treated group  (MedChemExpress)
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Effects of analyzed extracts on the area of cerebral thrombosis of zebrafish larvae. (A) Area of cerebral thrombosis and (B) representative images of the zebrafish larvae (5 days post-fertilization). Area inside the dashed lines indicates the observed area. Scale bar, 50 µm. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml <t>edaravone;</t> Pon, 3 µg/ml ponatinib.
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control edaravone eda  (MedChemExpress)
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Effects of analyzed extracts on the area of cerebral thrombosis of zebrafish larvae. (A) Area of cerebral thrombosis and (B) representative images of the zebrafish larvae (5 days post-fertilization). Area inside the dashed lines indicates the observed area. Scale bar, 50 µm. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml <t>edaravone;</t> Pon, 3 µg/ml ponatinib.
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edaravone  (Shanghai Yuanye Biochemicals)
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Effects of analyzed extracts on the area of cerebral thrombosis of zebrafish larvae. (A) Area of cerebral thrombosis and (B) representative images of the zebrafish larvae (5 days post-fertilization). Area inside the dashed lines indicates the observed area. Scale bar, 50 µm. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml <t>edaravone;</t> Pon, 3 µg/ml ponatinib.
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edaravone edv  (MedChemExpress)
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Effects of analyzed extracts on the area of cerebral thrombosis of zebrafish larvae. (A) Area of cerebral thrombosis and (B) representative images of the zebrafish larvae (5 days post-fertilization). Area inside the dashed lines indicates the observed area. Scale bar, 50 µm. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml <t>edaravone;</t> Pon, 3 µg/ml ponatinib.
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Effects of analyzed extracts on the area of cerebral thrombosis of zebrafish larvae. (A) Area of cerebral thrombosis and (B) representative images of the zebrafish larvae (5 days post-fertilization). Area inside the dashed lines indicates the observed area. Scale bar, 50 µm. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml <t>edaravone;</t> Pon, 3 µg/ml ponatinib.
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edaravone  (Macklin Inc)
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Effects of analyzed extracts on the area of cerebral thrombosis of zebrafish larvae. (A) Area of cerebral thrombosis and (B) representative images of the zebrafish larvae (5 days post-fertilization). Area inside the dashed lines indicates the observed area. Scale bar, 50 µm. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml <t>edaravone;</t> Pon, 3 µg/ml ponatinib.
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rapamycin  (MedChemExpress)
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Effects of analyzed extracts on the area of cerebral thrombosis of zebrafish larvae. (A) Area of cerebral thrombosis and (B) representative images of the zebrafish larvae (5 days post-fertilization). Area inside the dashed lines indicates the observed area. Scale bar, 50 µm. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml <t>edaravone;</t> Pon, 3 µg/ml ponatinib.
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Effects of analyzed extracts on the area of cerebral thrombosis of zebrafish larvae. (A) Area of cerebral thrombosis and (B) representative images of the zebrafish larvae (5 days post-fertilization). Area inside the dashed lines indicates the observed area. Scale bar, 50 µm. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml edaravone; Pon, 3 µg/ml ponatinib.

Journal: Biomedical Reports

Article Title: Toxicity evaluation and anti-ischemic stroke activity of selected natural extracts in zebrafish

doi: 10.3892/br.2026.2172

Figure Lengend Snippet: Effects of analyzed extracts on the area of cerebral thrombosis of zebrafish larvae. (A) Area of cerebral thrombosis and (B) representative images of the zebrafish larvae (5 days post-fertilization). Area inside the dashed lines indicates the observed area. Scale bar, 50 µm. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml edaravone; Pon, 3 µg/ml ponatinib.

Article Snippet: Ponatinib (Pon; lot no. #13771), aspirin (lot no. #323026) and edaravone (Eda; lot no. #35508) were purchased from MedChemExpress.

Techniques:

Effects of extracts on the total distance traveled of zebrafish larvae. Total distance traveled by zebrafish larvae (5 days post-fertilization) exposed to Pon and (A) CA, (B) MO, (C) CL, (D) PN and (E) CS extracts. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml edaravone; Pon, 3 µg/ml ponatinib.

Journal: Biomedical Reports

Article Title: Toxicity evaluation and anti-ischemic stroke activity of selected natural extracts in zebrafish

doi: 10.3892/br.2026.2172

Figure Lengend Snippet: Effects of extracts on the total distance traveled of zebrafish larvae. Total distance traveled by zebrafish larvae (5 days post-fertilization) exposed to Pon and (A) CA, (B) MO, (C) CL, (D) PN and (E) CS extracts. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml edaravone; Pon, 3 µg/ml ponatinib.

Article Snippet: Ponatinib (Pon; lot no. #13771), aspirin (lot no. #323026) and edaravone (Eda; lot no. #35508) were purchased from MedChemExpress.

Techniques:

Effect of extracts on the average speed of zebrafish larvae. Average speed of zebrafish larvae (5 days post-fertilization) exposed to Pon and (A) CA, (B) MO, (C) CL, (D) PN and (E) CS extracts. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml edaravone; Pon, 3 µg/ml ponatinib.

Journal: Biomedical Reports

Article Title: Toxicity evaluation and anti-ischemic stroke activity of selected natural extracts in zebrafish

doi: 10.3892/br.2026.2172

Figure Lengend Snippet: Effect of extracts on the average speed of zebrafish larvae. Average speed of zebrafish larvae (5 days post-fertilization) exposed to Pon and (A) CA, (B) MO, (C) CL, (D) PN and (E) CS extracts. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml edaravone; Pon, 3 µg/ml ponatinib.

Article Snippet: Ponatinib (Pon; lot no. #13771), aspirin (lot no. #323026) and edaravone (Eda; lot no. #35508) were purchased from MedChemExpress.

Techniques:

Representative swimming path of zebrafish larvae (5 days post-fertilization). CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml edaravone; Pon, 3 µg/ml ponatinib.

Journal: Biomedical Reports

Article Title: Toxicity evaluation and anti-ischemic stroke activity of selected natural extracts in zebrafish

doi: 10.3892/br.2026.2172

Figure Lengend Snippet: Representative swimming path of zebrafish larvae (5 days post-fertilization). CA, Centella asiatica ; MO, Moringa oleifera ; CL, Curcuma longa ; PN, Piper nigrum ; CS, Channa striata; Asp, 12.5 µg/ml aspirin; Eda, 12.5 µg/ml edaravone; Pon, 3 µg/ml ponatinib.

Article Snippet: Ponatinib (Pon; lot no. #13771), aspirin (lot no. #323026) and edaravone (Eda; lot no. #35508) were purchased from MedChemExpress.

Techniques: