Journal: International Journal of Molecular Sciences

Article Title: Three-Dimensional Bioprinting of an In Vitro Lung Model

doi: 10.3390/ijms24065852

Figure Lengend Snippet: Flow cytometry histograms. The histograms represent the cytometry of the WJ-MSC (sample C6) for the markers CD34, CD45, CD73, CD90, CD105, HLA-ABC, and HLA-DR, respectively (purple), as well as the isotypic control (gray). 1E2= 1 × 10 2 , 1E3= 1 × 10 3 , 1E4= 1 × 10 4 and, 1E5= 1 × 10 5 .

Article Snippet: Their characterization followed the International Society for Cell Therapy, which defines that for a cell to be considered an MSC, it must show adherence to the plastic substrate, express the cell surface markers CD73 (ecto-5-prime-nucleotidase), CD90 (Thy-1), and CD105 (endoglin) through flow cytometry, not express CD34 and CD45 (common leukocyte antigen) surface markers through flow cytometry, have multipotent differentiation capacity, that is, ability to differentiate into adipocytes, chondrocytes, and osteocytes, and present histocompatibility, which means express MHC class I (HLA-ABC) and not express MHC class II (HLA-DR).

Techniques: Flow Cytometry, Cytometry, Control

Journal: International Journal of Molecular Sciences

Article Title: Three-Dimensional Bioprinting of an In Vitro Lung Model

doi: 10.3390/ijms24065852

Figure Lengend Snippet: Cytometry panel.

Article Snippet: Their characterization followed the International Society for Cell Therapy, which defines that for a cell to be considered an MSC, it must show adherence to the plastic substrate, express the cell surface markers CD73 (ecto-5-prime-nucleotidase), CD90 (Thy-1), and CD105 (endoglin) through flow cytometry, not express CD34 and CD45 (common leukocyte antigen) surface markers through flow cytometry, have multipotent differentiation capacity, that is, ability to differentiate into adipocytes, chondrocytes, and osteocytes, and present histocompatibility, which means express MHC class I (HLA-ABC) and not express MHC class II (HLA-DR).

Techniques: Cytometry, Control

Journal: Cells

Article Title: The Generation of Human iPSC Lines from Three Individuals with Dravet Syndrome and Characterization of Neural Differentiation Markers in iPSC-Derived Ventral Forebrain Organoid Model

doi: 10.3390/cells12020339

Figure Lengend Snippet: The list of antibodies used for validation of pluripotency of reprogrammed iPSC lines and their differentiation potential.

Article Snippet: Primary antibodies mouse anti-Oct4 (Octamer-Binding Protein 4, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-SOX2 (SRY, Sex-Determining Region Y, Box 2, Invitrogen, Waltham, MA USA), rabbit anti-NANOG (Nanog Homeobox, Cell Signaling Technology, Danvers, MA, USA), mouse anti-SSEA4 (Stage-specific Embryonic Antigen 4, Millipore, Burlington, MA, USA), rabbit anti-Ki67 (Marker Of Proliferation Ki-67, Millipore, Burlington, MA, USA), mouse anti-CD73 (Ecto-5′-nucleotidase, Santa Cruz, Dallas, TX, USA), and mouse anti-SeV (Sendai virus, Invitrogen, Waltham, MA USA) were diluted in pre-incubation buffer and incubated at 4 °C overnight.

Techniques: Marker