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R&D Systems
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Thermo Fisher
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Millipore
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GenScript corporation
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Danaher Inc
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Assay Designs Inc
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Journal: bioRxiv
Article Title: Smooth muscle LRRC8A knockout preserves vascular function in AngII hypertension
doi: 10.1101/2025.05.08.652978
Figure Lengend Snippet: Antioxidant enzyme expression, proliferation and senescence are altered in abdominal aortae from LRRC8A KO mice. ( A ) CuZnSOD expression was not altered by LRRC8A KO or by AngII hypertension. ecSOD was increased by AngII only in KO vessels. Catalase and HO-1 expression were reduced in KO AngII hypertension compared to WT AngII. ( B ) iNOS and LRRC8A expression was enhanced by AngII hypertension in WT aortae. PCNA expression was increased in AngII hypertension ( p = 0.06), but this effect was reduced in KO vessels. ( C ) The senescence marker β-galactosidase (β-gal) increased by AngII hypertension in both WT and KO, but this effect was reduced in KO vessels. Data are mean ± SEM normalized to WT Sham. * p < 0.05 compared to WT Sham. † p < 0.05 (n=3 to 4).
Article Snippet: Antibodies were as follows: sGCα (#12605-1-AP), sGCβ (#19011-1-AP), cofilin (#66057-1-Ig) from Proteintech (Rosemont, IL), p-CPI-17 (#AB52174, Abcam, Cambridge, UK), CPI-17 (#sc-48406), ERM (#sc-271456), GAPDH (#sc-59540) from Santa Cruz Biotechnology (Dallas, TX), p-ERM (#3726), p-cofilin (#3313), Catalase (#14097), HO-1 (43966) from Cell Signaling Technology (Danvers, MA), CuZnSOD (#AF3418),
Techniques: Expressing, Marker