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(A) ASMC immunofluorescence staining for filamentous α-SMA, Igε-RI and Igε-RII. (B) Immune-blot of Igε-RI and Igε-RII siRNA treatment (48 hours) on protein expression; siCon: scrambled siRNA, U: untreated cells, GAPDH: house-keeping protein. <t>(C)</t> <t>IgE</t> induced <t>ECM.</t> (D) Collagen deposition (48 hours) and its modulation by siRNA targeting Igε-RI, Igε-RII or by Omalizumab. IgG served as control. Statistics: ANOVA. siCon: control siRNA; U: untreated cells.
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(A) ASMC immunofluorescence staining for filamentous α-SMA, Igε-RI and Igε-RII. (B) Immune-blot of Igε-RI and Igε-RII siRNA treatment (48 hours) on protein expression; siCon: scrambled siRNA, U: untreated cells, GAPDH: house-keeping protein. <t>(C)</t> <t>IgE</t> induced <t>ECM.</t> (D) Collagen deposition (48 hours) and its modulation by siRNA targeting Igε-RI, Igε-RII or by Omalizumab. IgG served as control. Statistics: ANOVA. siCon: control siRNA; U: untreated cells.
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(A) ASMC immunofluorescence staining for filamentous α-SMA, Igε-RI and Igε-RII. (B) Immune-blot of Igε-RI and Igε-RII siRNA treatment (48 hours) on protein expression; siCon: scrambled siRNA, U: untreated cells, GAPDH: house-keeping protein. <t>(C)</t> <t>IgE</t> induced <t>ECM.</t> (D) Collagen deposition (48 hours) and its modulation by siRNA targeting Igε-RI, Igε-RII or by Omalizumab. IgG served as control. Statistics: ANOVA. siCon: control siRNA; U: untreated cells.
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Image Search Results


(A) ASMC immunofluorescence staining for filamentous α-SMA, Igε-RI and Igε-RII. (B) Immune-blot of Igε-RI and Igε-RII siRNA treatment (48 hours) on protein expression; siCon: scrambled siRNA, U: untreated cells, GAPDH: house-keeping protein. (C) IgE induced ECM. (D) Collagen deposition (48 hours) and its modulation by siRNA targeting Igε-RI, Igε-RII or by Omalizumab. IgG served as control. Statistics: ANOVA. siCon: control siRNA; U: untreated cells.

Journal: PLoS ONE

Article Title: The Role of IgE-Receptors in IgE-Dependent Airway Smooth Muscle Cell Remodelling

doi: 10.1371/journal.pone.0056015

Figure Lengend Snippet: (A) ASMC immunofluorescence staining for filamentous α-SMA, Igε-RI and Igε-RII. (B) Immune-blot of Igε-RI and Igε-RII siRNA treatment (48 hours) on protein expression; siCon: scrambled siRNA, U: untreated cells, GAPDH: house-keeping protein. (C) IgE induced ECM. (D) Collagen deposition (48 hours) and its modulation by siRNA targeting Igε-RI, Igε-RII or by Omalizumab. IgG served as control. Statistics: ANOVA. siCon: control siRNA; U: untreated cells.

Article Snippet: To study the role of the two IgE receptors on IgE-induced ECM deposition, cells were pre-treated with 0, 1, or 5 µM of IgE receptor specific siRNAs (Santa Cruz Biotech) for 24 hours.

Techniques: Immunofluorescence, Staining, Expressing

Summary of TEVG studies that involved clinical trials and large animal models

Journal: Advanced Healthcare Materials

Article Title: Immunomodulation Strategies for the Successful Regeneration of a Tissue‐Engineered Vascular Graft

doi: 10.1002/adhm.202200045

Figure Lengend Snippet: Summary of TEVG studies that involved clinical trials and large animal models

Article Snippet: Human cadaveric SMC‐deposited ECM , Cell deposition and decellularization , 6 , Decellularized , In vivo (baboon model, arteriovenous access) , Patent for up to 6 months Patency rate was 88% No dilation and change in thickness for 24 weeks No sign of infection, calcification, aneurysmal dilation, and no substantial intimal hyperplasia , Niklason Lab/Humacyte , Dahl et al., 2011.

Techniques: Clinical Proteomics, In Vivo, Infection

Summary of TEVG studies that involved clinical trials and large animal models

Journal: Advanced Healthcare Materials

Article Title: Immunomodulation Strategies for the Successful Regeneration of a Tissue‐Engineered Vascular Graft

doi: 10.1002/adhm.202200045

Figure Lengend Snippet: Summary of TEVG studies that involved clinical trials and large animal models

Article Snippet: Human SMC‐deposited ECM , Cell deposition and decellularization , 6 , Decellularized , Phase 2 Clinical trial as arteriovenous access and ongoing Phase 3 trial , , Niklason Lab/Humacyte , Kirkto et al., 2019.

Techniques: Clinical Proteomics, In Vivo, Infection

Summary of TEVG studies that involved clinical trials and large animal models

Journal: Advanced Healthcare Materials

Article Title: Immunomodulation Strategies for the Successful Regeneration of a Tissue‐Engineered Vascular Graft

doi: 10.1002/adhm.202200045

Figure Lengend Snippet: Summary of TEVG studies that involved clinical trials and large animal models

Article Snippet: Allogeneic canine SMC‐deposited ECM , Cell deposition and decellularization , 3–4 , Autologous ECs , In vivo (canine carotid artery bypass graft) , Patency rate was 83% No sign of infection, calcification, aneurysmal dilation, and no intimal hyperplasia at anastomoses up to 1 year , Niklason Lab/Humacyte , Dahl et al., 2011.

Techniques: Clinical Proteomics, In Vivo, Infection