eclipse ni e upright fluorescence microscope (Nikon)
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eclipse ni e upright fluorescence microscope
Eclipse Ni E Upright Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59766 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ni-e+fluorescent+microscope/Objectives/pmc12863670-72-9-8
Average 99 stars, based on 59766 article reviews
Eclipse Ni E Upright Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59766 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+ni-e+fluorescent+microscope/Objectives/pmc12863670-72-9-8
Average 99 stars, based on 59766 article reviews
eclipse ni e upright fluorescence microscope - by Bioz Stars,
2026-10
99/100 stars
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![a Phase contrast and H&E staining of NC- and BA-MBOs. Three experiments were repeated with similar results. b Immunostaining of CK19 (green), CD31 (white), VIM (red) and Nucleus (blue; DAPI) in MBOs. Abundance of CK19 + cells ( n = 12 fields for NC or 14 fields for BA examined over 3 independent samples) and average size of gland-like structures ( n = 11 fields for NC or 7 fields for BA examined over 3 independent samples) were quantified (Mean ± SD). c Immunostaining of ZO1 (green), β-catenin (red), CK19 (white) and nucleus (blue; DAPI) in MBOs. Graphs show CK19 + cells exhibiting apical-basal polarity (Mean ± SD; n = 12 fields per 3 organoids). d Alcian Blue staining for MBOs. Mucin production in epithelium was determined by ratio of Alcian Blue + areas in CK19 + (from separately examined <t>immunofluorescence</t> data) areas (Mean ± SD; n = 15 fields [for NC] or 12 fields [for BA] examined over 3 independent samples). e Heatmap (left) and Volcano plot (right) of differentially expressed genes between NC- and BA-MBOs (up in BA: 350; down in BA: 851). f DAB staining of MBOs for MMP-7 and OPN. The staining intensities were quantified by imaging analysis (Mean ± SD; n = 12 fields per 3 organoids). g Representative plots and quantification of FACS for hybrid cells (pan-CK + /VIM + ) and cholangiocytes (pan-CK + /VIM − ) in Mono and MBO. The number and cell ratio were quantified (Mean ± SD; n = 6). For b – d , f , g , the p -values were shown in plots and were determined using two-tailed Welch’s t -test.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1497/pmc12271497/pmc12271497__41467_2025_61442_Fig2_HTML.jpg)
