Journal: bioRxiv

Article Title: A Transient Increase in Cardiomyocyte Protein O-GlcNAcylation Enhances Susceptibility to Pressure Overload-Induced Cardiac Remodeling

doi: 10.1101/2025.06.04.657956

Figure Lengend Snippet: Schematic showing the experimental timeline and procedures for the dnOGAh ON/OFF-TAC paradigm used in this study—from induction of dnOGA transgene via DOX, switching the transgene “off” via removal of DOX, and TAC procedure, to subsequent echocardiography and tissue harvesting for downstream analyses.

Article Snippet: At the prespecified timepoints shown in the experimental schema ( ) after 8 weeks of TAC/Sham, transthoracic echocardiography was performed on all mice in the study, as previously described., In brief, the mice were anesthetized via 2% isoflurane induction chamber inhalation then placed in a supine position on a heated echocardiography platform (Vevo integrated rail system, VisualSonics) set at 37°C to maintain body temperature; sedation was maintained via nose cone inhalation of 1.0-1.5% isoflurane which allowed for heart rate maintenance of ∼400 beats / min. Fur from the chest was removed via topical depilatory cream, and then the mice were then placed in a left-lateral-decubitus position.

Techniques:

Journal: bioRxiv

Article Title: A Transient Increase in Cardiomyocyte Protein O-GlcNAcylation Enhances Susceptibility to Pressure Overload-Induced Cardiac Remodeling

doi: 10.1101/2025.06.04.657956

Figure Lengend Snippet:

Article Snippet: At the prespecified timepoints shown in the experimental schema ( ) after 8 weeks of TAC/Sham, transthoracic echocardiography was performed on all mice in the study, as previously described., In brief, the mice were anesthetized via 2% isoflurane induction chamber inhalation then placed in a supine position on a heated echocardiography platform (Vevo integrated rail system, VisualSonics) set at 37°C to maintain body temperature; sedation was maintained via nose cone inhalation of 1.0-1.5% isoflurane which allowed for heart rate maintenance of ∼400 beats / min. Fur from the chest was removed via topical depilatory cream, and then the mice were then placed in a left-lateral-decubitus position.

Techniques:

Journal: bioRxiv

Article Title: A Transient Increase in Cardiomyocyte Protein O-GlcNAcylation Enhances Susceptibility to Pressure Overload-Induced Cardiac Remodeling

doi: 10.1101/2025.06.04.657956

Figure Lengend Snippet: A) Normalized ratio of wet-dry lung weight to TL for Con-TAC and ON/OFF-TAC mice compared to age-matched Sham controls (Con-Sham and ON/OFF-Sham) ( n ≥ 29). B) Normalized ratio of wet-dry lung weight to TL for Con-TAC and ON/OFF-TAC mice compared to age-matched Sham controls (Con-Sham and ON/OFF-Sham) split by sex. C-G) Cardiac function measures via echocardiography split by sex for: cardiac systolic function as measured by LV ejection fraction from M-mode LV traces ( C ), stroke volume from M-mode LV traces ( D ), left ventricular internal dimension at diastole (LVIDd) ( E ), cardiac wall thickening as measured by LV posterior wall thickness at diastole (LVPWd) ( F ), and inter-ventricular septum thickness at diastole (IVSd) ( G ). Statistical significance was based on two-way ANOVA followed by Tukey’s multiple comparisons test (* p < 0.05, ** p < 0.01, *** p < 0.005). All calculated and analysis data are mean ± S.E.M.

Article Snippet: At the prespecified timepoints shown in the experimental schema ( ) after 8 weeks of TAC/Sham, transthoracic echocardiography was performed on all mice in the study, as previously described., In brief, the mice were anesthetized via 2% isoflurane induction chamber inhalation then placed in a supine position on a heated echocardiography platform (Vevo integrated rail system, VisualSonics) set at 37°C to maintain body temperature; sedation was maintained via nose cone inhalation of 1.0-1.5% isoflurane which allowed for heart rate maintenance of ∼400 beats / min. Fur from the chest was removed via topical depilatory cream, and then the mice were then placed in a left-lateral-decubitus position.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: Three-dimensional printing of mitral valve models using echocardiographic data improves the knowledge of cardiology fellow physicians in training

doi: 10.3389/fcvm.2023.1307994

Figure Lengend Snippet: Three-dimensional echocardiography image of the mitral valve showing the normal shape of the annulus, both leaflets and the corresponding segments. (LAA, left atrial appendage; ALC, antero-lateral commissure; PMC, postero-medial commissure).

Article Snippet: Imaging of the MV and the acquisition of 3D data sets were completed using a (t7-1) transesophageal probe and (iE-33) echocardiography platforms (Philips, Eindhoven Netherlands).

Techniques:

Journal: Physiological Research

Article Title: Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Myocardial Injury Through TLR4-NLRP3 Pathway

doi: 10.33549/physiolres.934928

Figure Lengend Snippet: Exogenous H2S amelio-rates myocardial tissue injury. (A) Representative M-mode images from Control, LPS, and LPS+NaHS groups. (B) Represen-tative HE-stained left ventricular sections (scale bar = 50 μm). (C) The changes of left ventri-cular ejection fraction (LVEF). (D) Pathological score of the HE-stained left ventricular sec-tions. (E) H2S levels in the SIMD mice model plasma. (F) cTnI levels in the SIMD mice model plasma. (G) TNF-α levels in the SIMD mice model plasma. (H) IL-1β levels in the SIMD mice model plasma. (I–J) Represen-tative Western blots and quan-tification of TLR4 and NLRP3 protein expression in heart tissues. β-actin was used as the internal control. n=8 in every mice model group. n=5 in every Western blot group. Results are means ± SEM. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. P<0.05 was considered significant.

Article Snippet: To evaluate left ventricular function, mouse two-dimensional echocardiography was performed using a VisualSonics Vevo 770 platform.

Techniques: Control, Staining, Clinical Proteomics, Western Blot, Expressing

Journal: Physiological Research

Article Title: Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Myocardial Injury Through TLR4-NLRP3 Pathway

doi: 10.33549/physiolres.934928

Figure Lengend Snippet: NLRP3 inhibitor, MCC950, attenuates LPS-induced myocardial injury. (A) Representative M-mode images from LPS, LPS+MCC950 and LPS+NaHS+MCC950 groups (B) Representative HE-stained left ventricular sections (scale bar = 50 μm). (C) The changes of left ventricular ejection fraction (LVEF). (D) Pathological score of the HE-stained left ventricular sections. (E) cTnI levels in the SIMD mice model plasma. (F) TNF-α levels in the SIMD mice model plasma. (G) IL-1β levels in the SIMD mice model plasma. n=8 in each group. Results are means ± SEM. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. P<0.05 was considered significant.

Article Snippet: To evaluate left ventricular function, mouse two-dimensional echocardiography was performed using a VisualSonics Vevo 770 platform.

Techniques: Staining, Clinical Proteomics

Journal: Physiological Research

Article Title: Hydrogen Sulfide Alleviates Lipopolysaccharide-Induced Myocardial Injury Through TLR4-NLRP3 Pathway

doi: 10.33549/physiolres.934928

Figure Lengend Snippet: NaHS exerts protective effects mainly through the TLR4-NLRP3 signaling axis (A) Representative M-mode images from WT, WT+LPS, TLR4−/−+LPS, TLR4−/−+LPS+NaHS groups (B) Representative HE-stained left ventricular sections (scale bar = 50 μm). (C) The changes of left ventricular ejection fraction (LVEF). (D) Pathological score of the HE-stained left ventricular sections. (E) cTnI levels in the SIMD mice model plasma. (F) TNF-α levels in the SIMD mice model plasma. (G) IL-1β levels in the SIMD mice model plasma. n=8 in each group. Results are means ± SEM. * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001. P<0.05 was considered significant.

Article Snippet: To evaluate left ventricular function, mouse two-dimensional echocardiography was performed using a VisualSonics Vevo 770 platform.

Techniques: Staining, Clinical Proteomics