Journal: Biosensors

Article Title: Fast Detection of 2,4,6-Trinitrotoluene (TNT) at ppt Level by a Laser-Induced Immunofluorometric Biosensor

doi: 10.3390/bios10080089

Figure Lengend Snippet: A biosensor based on an immunofluorometric assay: The monoclonal antibody A1.1.1 against 2,4,6-trinitrotoluene (TNT) is labeled with the fluorescent dye Dy-654. This conjugate is briefly preincubated with the sample and pumped through the antigen-coated affinity column, which traps free antibody. Only antibody-TNT complexes reach the highly sensitive, laser-induced fluorescence (LIF) detector.

Article Snippet: In order to prepare the 4.54 mM Dy-654-NHS labeling solution, 0.2 mg of the NHS ester (0.18 µM) were dissolved in 40 µL of dry, amine-free DMF (labeling grade, Lumiprobe) and aliquoted to 10 µL portions and stored in the dark at −18 °C.

Techniques: Labeling, Affinity Column, Fluorescence

Journal: Biosensors

Article Title: Fast Detection of 2,4,6-Trinitrotoluene (TNT) at ppt Level by a Laser-Induced Immunofluorometric Biosensor

doi: 10.3390/bios10080089

Figure Lengend Snippet: Smoothed MALDI-TOF MS spectra of the antibody A1.1.1 and the conjugate with the fluorescent dye Dy-654 (top) and structure of the label Dy-654-COOH (MW 1007.08 g/mol, Dyomics).

Article Snippet: In order to prepare the 4.54 mM Dy-654-NHS labeling solution, 0.2 mg of the NHS ester (0.18 µM) were dissolved in 40 µL of dry, amine-free DMF (labeling grade, Lumiprobe) and aliquoted to 10 µL portions and stored in the dark at −18 °C.

Techniques:

Journal: Biosensors

Article Title: Fast Detection of 2,4,6-Trinitrotoluene (TNT) at ppt Level by a Laser-Induced Immunofluorometric Biosensor

doi: 10.3390/bios10080089

Figure Lengend Snippet: Determination of the limit of detection (LOD) (3s) and LOQ (10s) for the fluorescence label Dy-654-COOH (top) and calibration line in the linear range from 4–1000 pM (bottom).

Article Snippet: In order to prepare the 4.54 mM Dy-654-NHS labeling solution, 0.2 mg of the NHS ester (0.18 µM) were dissolved in 40 µL of dry, amine-free DMF (labeling grade, Lumiprobe) and aliquoted to 10 µL portions and stored in the dark at −18 °C.

Techniques: Fluorescence