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dpx2 cell  (Puracyp Inc)

 
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    Structured Review

    Puracyp Inc dpx2 cell
    Dpx2 Cell, supplied by Puracyp Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dpx2+cells/dpx2+cells/us12297192-226-5-9
    Average 90 stars, based on 1 article reviews
    dpx2 cell - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Stable Transfection:

    Article Title: Metabolism of Beclomethasone Dipropionate by Cytochrome P450 3A Enzymes
    Article Snippet: A549 cells (human lung adenocarcinoma) (ATCC, Manassas, VA) were cultured in Dulbecco’s modified Eagle’s medium containing 5% fetal bovine serum (Life Technologies, Grand Island, NY). .. DPX2 cells (HepG2 background with human PXR stably overexpressed to drive the expression of a CYP3A4 reporter gene construct by PXR agonists) were provided by Dr. Judy Raucy (Puracyp Inc., Carlsbad, CA). .. DPX2 cells were cultured in Puracyp media (Puracyp Inc.) ( Lamb et al., 2010 ).

    Article Title: Comparative analysis of human CYP3A4 and rat CYP3A1 induction and relevant gene expression by bisphenol A and diethylstilbestrol: implications for toxicity testing paradigms.
    Article Snippet: Bisphenol A (BPA) and diethylstilbestrol (DES) are endocrine-disrupting chemicals that interact with the human pregnane X receptor (PXR).. CYP3A4 enzyme is essential in the hydroxylation of steroid hormones and is regulated by PXR.. In the present study, human and rat hepatoma cell lines were exposed to BPA and DES.

    Article Title: Chemical basis of pregnane X receptor activators in the herbal supplement Gancao (licorice)
    Article Snippet: Midazolam (MDZ) and its primary metabolite 1′-hydroxymidazolam (1′-OHMDZ) were obtained from Akorn Pharmaceuticals (Lake Forest, IL, USA) and Toronto Research Chemicals (Toronto, Ontario, Canada), respectively. .. DPX2 cells (Puracyp, Inc; Carlsbad, CA, USA) were used for screening PXR activators, which were derived from HepG2 cells and stably transfected with hPXR and a luciferase-linked CYP3A4 promoter. ..

    Expressing:

    Article Title: Metabolism of Beclomethasone Dipropionate by Cytochrome P450 3A Enzymes
    Article Snippet: A549 cells (human lung adenocarcinoma) (ATCC, Manassas, VA) were cultured in Dulbecco’s modified Eagle’s medium containing 5% fetal bovine serum (Life Technologies, Grand Island, NY). .. DPX2 cells (HepG2 background with human PXR stably overexpressed to drive the expression of a CYP3A4 reporter gene construct by PXR agonists) were provided by Dr. Judy Raucy (Puracyp Inc., Carlsbad, CA). .. DPX2 cells were cultured in Puracyp media (Puracyp Inc.) ( Lamb et al., 2010 ).

    Article Title: Comparative analysis of human CYP3A4 and rat CYP3A1 induction and relevant gene expression by bisphenol A and diethylstilbestrol: implications for toxicity testing paradigms.
    Article Snippet: Bisphenol A (BPA) and diethylstilbestrol (DES) are endocrine-disrupting chemicals that interact with the human pregnane X receptor (PXR).. CYP3A4 enzyme is essential in the hydroxylation of steroid hormones and is regulated by PXR.. In the present study, human and rat hepatoma cell lines were exposed to BPA and DES.

    Construct:

    Article Title: Metabolism of Beclomethasone Dipropionate by Cytochrome P450 3A Enzymes
    Article Snippet: A549 cells (human lung adenocarcinoma) (ATCC, Manassas, VA) were cultured in Dulbecco’s modified Eagle’s medium containing 5% fetal bovine serum (Life Technologies, Grand Island, NY). .. DPX2 cells (HepG2 background with human PXR stably overexpressed to drive the expression of a CYP3A4 reporter gene construct by PXR agonists) were provided by Dr. Judy Raucy (Puracyp Inc., Carlsbad, CA). .. DPX2 cells were cultured in Puracyp media (Puracyp Inc.) ( Lamb et al., 2010 ).

    Article Title: Comparative analysis of human CYP3A4 and rat CYP3A1 induction and relevant gene expression by bisphenol A and diethylstilbestrol: implications for toxicity testing paradigms.
    Article Snippet: Bisphenol A (BPA) and diethylstilbestrol (DES) are endocrine-disrupting chemicals that interact with the human pregnane X receptor (PXR).. CYP3A4 enzyme is essential in the hydroxylation of steroid hormones and is regulated by PXR.. In the present study, human and rat hepatoma cell lines were exposed to BPA and DES.

    Luciferase:

    Article Title: Comparative analysis of human CYP3A4 and rat CYP3A1 induction and relevant gene expression by bisphenol A and diethylstilbestrol: implications for toxicity testing paradigms.
    Article Snippet: Bisphenol A (BPA) and diethylstilbestrol (DES) are endocrine-disrupting chemicals that interact with the human pregnane X receptor (PXR).. CYP3A4 enzyme is essential in the hydroxylation of steroid hormones and is regulated by PXR.. In the present study, human and rat hepatoma cell lines were exposed to BPA and DES.

    Article Title: Chemical basis of pregnane X receptor activators in the herbal supplement Gancao (licorice)
    Article Snippet: Midazolam (MDZ) and its primary metabolite 1′-hydroxymidazolam (1′-OHMDZ) were obtained from Akorn Pharmaceuticals (Lake Forest, IL, USA) and Toronto Research Chemicals (Toronto, Ontario, Canada), respectively. .. DPX2 cells (Puracyp, Inc; Carlsbad, CA, USA) were used for screening PXR activators, which were derived from HepG2 cells and stably transfected with hPXR and a luciferase-linked CYP3A4 promoter. ..

    other:

    Article Title: Activation Status of the Pregnane X Receptor Influences Vemurafenib Availability in Humanized Mouse Models
    Article Snippet: Cells stably transfected with the PXR gene and a reporter construct with luciferase under the control of PXR-responsive elements of the CYP3A4 promoter (DPX2 cells, Puracyp Inc., Carlsbad, CA, USA) were treated with a range of concentrations of vemurafenib (0.06, 0.17, 0.51, 1.54, 4.62, 13.89, 41.67 and 125 μM) in triplicate for 48 hours and assayed for luminescence and fluorescence as per the manufacturer’s instructions.

    Article Title: Optimization of Benzoxazinorifamycins to Minimize hPXR Activation for the Treatment of Tuberculosis and HIV Coinfection.
    Article Snippet: These cells are available from Puracyp, Inc., as part of an hPXR activation assay system (Product DPX2-96-001) which also contains the materials needed to perform the reporter assays in a 96-well format.

    Article Title: Metabolism of Beclomethasone Dipropionate by Cytochrome P450 3A Enzymes
    Article Snippet: The authors thank Dr. Christopher Orton for initiating this research, Dr. Judy Raucy (Puracyp Inc.) for the kind donation of DPX2 cells, Dr. John G. Lamb for assistance in culturing the DPX2 cells, and Dr. Roger Gaedigk for the CYP3A5 probe and primer sequences.

    Article Title: The Chinese Herbal Medicine Sophora flavescens Activates Pregnane X Receptor
    Article Snippet: We thank Puracyp, Inc., for providing DPX2 cells and Biopredic International for providing HepaRG cells.

    Article Title: Activation status of the Pregnane X Receptor (PXR) influences Vemurafenib availability in humanized mouse models
    Article Snippet: PXR-transactivation assay Cells stably transfected with the PXR gene and a reporter construct with luciferase under the control of PXR-responsive elements of the CYP3A4 promoter (DPX2 cells, Puracyp Inc., Carlsbad, CA, USA) were treated with a range of concentrations of vemurafenib (0.06, 0.17, 0.51, 1.54, 4.62, 13.89, 41.67 and 125 μM) in triplicate for 48 hours and assayed for luminescence and fluorescence as per the manufacturer’s instructions.

    Derivative Assay:

    Article Title: Chemical basis of pregnane X receptor activators in the herbal supplement Gancao (licorice)
    Article Snippet: Midazolam (MDZ) and its primary metabolite 1′-hydroxymidazolam (1′-OHMDZ) were obtained from Akorn Pharmaceuticals (Lake Forest, IL, USA) and Toronto Research Chemicals (Toronto, Ontario, Canada), respectively. .. DPX2 cells (Puracyp, Inc; Carlsbad, CA, USA) were used for screening PXR activators, which were derived from HepG2 cells and stably transfected with hPXR and a luciferase-linked CYP3A4 promoter. ..

    Transfection:

    Article Title: Chemical basis of pregnane X receptor activators in the herbal supplement Gancao (licorice)
    Article Snippet: Midazolam (MDZ) and its primary metabolite 1′-hydroxymidazolam (1′-OHMDZ) were obtained from Akorn Pharmaceuticals (Lake Forest, IL, USA) and Toronto Research Chemicals (Toronto, Ontario, Canada), respectively. .. DPX2 cells (Puracyp, Inc; Carlsbad, CA, USA) were used for screening PXR activators, which were derived from HepG2 cells and stably transfected with hPXR and a luciferase-linked CYP3A4 promoter. ..



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    Gancao extract activates hPXR. Cell-based hPXR reporter assays were conducted using <t>DPX2</t> cells. (A) Effect of Gancao extract on hPXR activation. Gancao extract was diluted 100-fold. RIF (10 μmol/L) was used as a positive control for hPXR activation. (B) A dose-dependent hPXR activation by Gancao extract (100- to 1000-fold dilution). The data in control groups were set as 1. All data are expressed as means ± SEM ( n = 3). ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. control. Abbreviations: hPXR, human pregnane X receptor; RIF, rifampicin; SEM, standard error of the mean.
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    Gancao extract activates hPXR. Cell-based hPXR reporter assays were conducted using DPX2 cells. (A) Effect of Gancao extract on hPXR activation. Gancao extract was diluted 100-fold. RIF (10 μmol/L) was used as a positive control for hPXR activation. (B) A dose-dependent hPXR activation by Gancao extract (100- to 1000-fold dilution). The data in control groups were set as 1. All data are expressed as means ± SEM ( n = 3). ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. control. Abbreviations: hPXR, human pregnane X receptor; RIF, rifampicin; SEM, standard error of the mean.

    Journal: Liver Research

    Article Title: Chemical basis of pregnane X receptor activators in the herbal supplement Gancao (licorice) ☆

    doi: 10.1016/j.livres.2022.11.007

    Figure Lengend Snippet: Gancao extract activates hPXR. Cell-based hPXR reporter assays were conducted using DPX2 cells. (A) Effect of Gancao extract on hPXR activation. Gancao extract was diluted 100-fold. RIF (10 μmol/L) was used as a positive control for hPXR activation. (B) A dose-dependent hPXR activation by Gancao extract (100- to 1000-fold dilution). The data in control groups were set as 1. All data are expressed as means ± SEM ( n = 3). ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 vs. control. Abbreviations: hPXR, human pregnane X receptor; RIF, rifampicin; SEM, standard error of the mean.

    Article Snippet: DPX2 cells (Puracyp, Inc; Carlsbad, CA, USA) were used for screening PXR activators, which were derived from HepG2 cells and stably transfected with hPXR and a luciferase-linked CYP3A4 promoter.

    Techniques: Activation Assay, Positive Control, Control

    Screening of Gancao phytoconstituents for PXR activators. DPX2 cells were used for hPXR reporter assays. (A) Effects of the identified compounds (10 μmol/L each) from Gancao extract on hPXR activation. RIF (10 μmol/L) was used as a positive control. All data are expressed as means ± SEM ( n = 3). ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001 vs. control (set as 1). (B) Dose responses of GZ, GA, and glabridin on hPXR activation. The data in RIF group at 100 μmol/L were set as 1. All data are expressed as means ( n = 3). Abbreviations: GA, glycyrrhetinic acid; GZ, glycyrrhizin; hPXR, human pregnane X receptor; RIF, rifampicin; SEM, standard error of the mean.

    Journal: Liver Research

    Article Title: Chemical basis of pregnane X receptor activators in the herbal supplement Gancao (licorice) ☆

    doi: 10.1016/j.livres.2022.11.007

    Figure Lengend Snippet: Screening of Gancao phytoconstituents for PXR activators. DPX2 cells were used for hPXR reporter assays. (A) Effects of the identified compounds (10 μmol/L each) from Gancao extract on hPXR activation. RIF (10 μmol/L) was used as a positive control. All data are expressed as means ± SEM ( n = 3). ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001 vs. control (set as 1). (B) Dose responses of GZ, GA, and glabridin on hPXR activation. The data in RIF group at 100 μmol/L were set as 1. All data are expressed as means ( n = 3). Abbreviations: GA, glycyrrhetinic acid; GZ, glycyrrhizin; hPXR, human pregnane X receptor; RIF, rifampicin; SEM, standard error of the mean.

    Article Snippet: DPX2 cells (Puracyp, Inc; Carlsbad, CA, USA) were used for screening PXR activators, which were derived from HepG2 cells and stably transfected with hPXR and a luciferase-linked CYP3A4 promoter.

    Techniques: Activation Assay, Positive Control, Control

    DPX2 cells were used to calculate the EC50 (8.13 μM) and EMAX (8.54-fold) for vemurafenib mediated trans-activation of PXR

    Journal: Cancer research

    Article Title: Activation status of the Pregnane X Receptor (PXR) influences Vemurafenib availability in humanized mouse models

    doi: 10.1158/0008-5472.CAN-15-1454

    Figure Lengend Snippet: DPX2 cells were used to calculate the EC50 (8.13 μM) and EMAX (8.54-fold) for vemurafenib mediated trans-activation of PXR

    Article Snippet: We tested the capacity of crystalline vemurafenib to activate a PXR-driven luciferase reporter construct in the DPX2 cell line (Puracyp Inc.).

    Techniques: Activation Assay