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Western blot analysis of the protein levels of NOD-like receptor pyrin domain-containing 3, associated speck-like protein containing a CARD, and procaspase-1 in H9c2 cells after hypoxia/re-oxygenation treatment, with or without acetylcysteine or nicotinamide adenine dinucleotide oxidized form <t>(NAD</t> + ) ( n = 3). NLRP3: NOD-like receptor pyrin domain-containing 3; ASC: Associated speck-like protein containing a CARD; <t>GAPDH:</t> <t>Glyceraldehyde-3-phosphate</t> dehydrogenase; H/R: Hypoxia/re-oxygenation; NAD: Nicotinamide adenine dinucleotide; NAD + : Nicotinamide adenine dinucleotide oxidized form.
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Western blot analysis of the protein levels of NOD-like receptor pyrin domain-containing 3, associated speck-like protein containing a CARD, and procaspase-1 in H9c2 cells after hypoxia/re-oxygenation treatment, with or without acetylcysteine or nicotinamide adenine dinucleotide oxidized form <t>(NAD</t> + ) ( n = 3). NLRP3: NOD-like receptor pyrin domain-containing 3; ASC: Associated speck-like protein containing a CARD; <t>GAPDH:</t> <t>Glyceraldehyde-3-phosphate</t> dehydrogenase; H/R: Hypoxia/re-oxygenation; NAD: Nicotinamide adenine dinucleotide; NAD + : Nicotinamide adenine dinucleotide oxidized form.
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Western blot analysis of the protein levels of NOD-like receptor pyrin domain-containing 3, associated speck-like protein containing a CARD, and procaspase-1 in H9c2 cells after hypoxia/re-oxygenation treatment, with or without acetylcysteine or nicotinamide adenine dinucleotide oxidized form <t>(NAD</t> + ) ( n = 3). NLRP3: NOD-like receptor pyrin domain-containing 3; ASC: Associated speck-like protein containing a CARD; <t>GAPDH:</t> <t>Glyceraldehyde-3-phosphate</t> dehydrogenase; H/R: Hypoxia/re-oxygenation; NAD: Nicotinamide adenine dinucleotide; NAD + : Nicotinamide adenine dinucleotide oxidized form.
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Western blot analysis of the protein levels of NOD-like receptor pyrin domain-containing 3, associated speck-like protein containing a CARD, and procaspase-1 in H9c2 cells after hypoxia/re-oxygenation treatment, with or without acetylcysteine or nicotinamide adenine dinucleotide oxidized form <t>(NAD</t> + ) ( n = 3). NLRP3: NOD-like receptor pyrin domain-containing 3; ASC: Associated speck-like protein containing a CARD; <t>GAPDH:</t> <t>Glyceraldehyde-3-phosphate</t> dehydrogenase; H/R: Hypoxia/re-oxygenation; NAD: Nicotinamide adenine dinucleotide; NAD + : Nicotinamide adenine dinucleotide oxidized form.
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Western blot analysis of the protein levels of NOD-like receptor pyrin domain-containing 3, associated speck-like protein containing a CARD, and procaspase-1 in H9c2 cells after hypoxia/re-oxygenation treatment, with or without acetylcysteine or nicotinamide adenine dinucleotide oxidized form (NAD + ) ( n = 3). NLRP3: NOD-like receptor pyrin domain-containing 3; ASC: Associated speck-like protein containing a CARD; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; H/R: Hypoxia/re-oxygenation; NAD: Nicotinamide adenine dinucleotide; NAD + : Nicotinamide adenine dinucleotide oxidized form.

Journal: World Journal of Cardiology

Article Title: Nicotinamide adenine dinucleotide inhibits the production of reactive oxygen species and myocardial cell pyroptosis caused by hypoxia/re-oxygenation injury

doi: 10.4330/wjc.v18.i1.114108

Figure Lengend Snippet: Western blot analysis of the protein levels of NOD-like receptor pyrin domain-containing 3, associated speck-like protein containing a CARD, and procaspase-1 in H9c2 cells after hypoxia/re-oxygenation treatment, with or without acetylcysteine or nicotinamide adenine dinucleotide oxidized form (NAD + ) ( n = 3). NLRP3: NOD-like receptor pyrin domain-containing 3; ASC: Associated speck-like protein containing a CARD; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; H/R: Hypoxia/re-oxygenation; NAD: Nicotinamide adenine dinucleotide; NAD + : Nicotinamide adenine dinucleotide oxidized form.

Article Snippet: NAD + (HY-B0445, MedChemExpress, NJ, United States) was dissolved in phosphate-buffered solution (PBS; KeyGEN BioTECH, Nanjing, China) to prepare a 100 mmol/L stock solution.

Techniques: Western Blot