Journal: STAR Protocols

Article Title: Protocol for differentiation and efficient AAV-mediated gene delivery to hiPSC-derived microglia for functional studies

doi: 10.1016/j.xpro.2026.104455

Figure Lengend Snippet: Stepwise overview of the transduction procedure Depending on viral titer, microplate format and number of wells to be transduced AAV can be pre-diluted or added straight to freshly made medium containing doxorubicin for subsequent overnight incubation.

Article Snippet: Doxorubicin hydrochloride , Thermo Fisher Scientific , J64000-MA.

Techniques: Transduction, Incubation

Journal: STAR Protocols

Article Title: Protocol for differentiation and efficient AAV-mediated gene delivery to hiPSC-derived microglia for functional studies

doi: 10.1016/j.xpro.2026.104455

Figure Lengend Snippet: Overview of AAV titration experiment and expected results AAV particles were diluted to final concentration in cell medium containing 300 nM doxorubicin and applied (using a full medium replacement) to hiMG at indicated concentrations (vg/mL). Fluorescence imaging of mScarlet expression was performed in live cultures 5 days post transduction. Optimal concentration was found to be around 6–8 × 10 8 vg/mL.

Article Snippet: Doxorubicin hydrochloride , Thermo Fisher Scientific , J64000-MA.

Techniques: Titration, Concentration Assay, Fluorescence, Imaging, Expressing, Transduction

Journal: STAR Protocols

Article Title: Protocol for preparing pH-sensitive Mg-DOX liposomes by remote loading for treatment of acidic tumors

doi: 10.1016/j.xpro.2026.104560

Figure Lengend Snippet: Preparation of MgAc 2 gradient liposomes and visual observation of the liposomes remote loaded with DOX (A) Platform illustrating the buffer exchange of MgAc 2 liposomes with 0.9% NaCl using a PD10 desalting column. (B) Photograph showing the color of MgAc 2 gradient liposomes, DOX·HCl and NaHCO 3 mixture stored at 25 °C for 5 min. (C) Photograph showing the color of the MgAc 2 gradient liposomes, DOX·HCl and NaHCO 3 mixture heated at 60 °C for 10 min.

Article Snippet: Doxorubicin Hydrochloride (DOX·HCl), USP grade , Shanghai yuanye Bio-Technology Co., Ltd. , CAS: 25316-40-9.

Techniques: Liposomes, Buffer Exchange

Journal: STAR Protocols

Article Title: Protocol for preparing pH-sensitive Mg-DOX liposomes by remote loading for treatment of acidic tumors

doi: 10.1016/j.xpro.2026.104560

Figure Lengend Snippet: Characterization of Mg-DOX liposomes for particle size and Zeta potential, phospholipid concentration, and DOX concentration (A) Size distribution of the Mg-DOX liposomes measured by DLS method. (B) Cryo-TEM micrograph of the Mg-DOX liposomes. Scale bar: 100 nm. (C) Standard curve of the Stewart’s assay of DSPC concentration. (D) Standard curve of the spectrophotometric determination of DOX·HCl concentration with the microplate reader.

Article Snippet: Doxorubicin Hydrochloride (DOX·HCl), USP grade , Shanghai yuanye Bio-Technology Co., Ltd. , CAS: 25316-40-9.

Techniques: Liposomes, Zeta Potential Analyzer, Concentration Assay