Journal: Materials Today Bio

Article Title: Identification of a myosin 1B-binding aptamer for fluorescence imaging and targeted therapy of esophageal squamous cell carcinoma

doi: 10.1016/j.mtbio.2026.102867

Figure Lengend Snippet: Selection and identification of DNA aptamers specific to ESCC. (A) Flow cytometry assays of the binding of the selected libraries to KYSE30 and Het-1A cells. (B) Flow cytometry assays of the binding of Z1-6 aptamer candidates to KYSE30 and Het-1A cells. (C) The binding signal-to-background ratio (SBR) calculation in B. Fluorescence intensity of random ssDNA was used as the background signal. (D) Confocal imaging of KYSE30 cells incubated with FAM-labeled Z1, Z2, and Z4 aptamers. The scale bar is 50 μm. (E) Determination of dissociation constant (K d ) of Z1, Z2, and Z4 aptamers for KYSE30 cells by flow cytometry. FAM-labeled random ssDNA was used as the negative control.

Article Snippet: To investigate the Dox loading capacity, Dox was fixed at 2 μM, and various aptamer-to-Dox ratios (0, 1:20, 1:15, 1:10, 1:7.5, 1:5, 1:3, and 1:2) were prepared as above mentioned, and Dox fluorescence was examined by F-7000 fluorescence spectrometer (Hitachi, Japan).

Techniques: Selection, Flow Cytometry, Binding Assay, Fluorescence, Imaging, Incubation, Labeling, Negative Control

Journal: Materials Today Bio

Article Title: Identification of a myosin 1B-binding aptamer for fluorescence imaging and targeted therapy of esophageal squamous cell carcinoma

doi: 10.1016/j.mtbio.2026.102867

Figure Lengend Snippet: Flow cytometry assays of the binding of Z1 (blue), Z2 (orange), and Z4 (green) aptamers to different cell lines. Random ssDNA (red) was used as the control probe. Aptamers and random ssDNA were labeled with FAM group for collecting fluorescence signals.

Article Snippet: To investigate the Dox loading capacity, Dox was fixed at 2 μM, and various aptamer-to-Dox ratios (0, 1:20, 1:15, 1:10, 1:7.5, 1:5, 1:3, and 1:2) were prepared as above mentioned, and Dox fluorescence was examined by F-7000 fluorescence spectrometer (Hitachi, Japan).

Techniques: Flow Cytometry, Binding Assay, Control, Labeling, Fluorescence

Journal: Materials Today Bio

Article Title: Identification of a myosin 1B-binding aptamer for fluorescence imaging and targeted therapy of esophageal squamous cell carcinoma

doi: 10.1016/j.mtbio.2026.102867

Figure Lengend Snippet: Fluorescence imaging of ESCC tissues with Cy5-labeled aptamer probes. (A) Representative images of ESCC and adjacent tissues stained with Cy5-labeled Z2 and Z4-6 aptamer probes. Cy5-labeled random ssDNA was used as the control probe. Scale bar is 50 μm. (B) Quantitative fluorescence intensity of ESCC and adjacent tissues stained with Cy5-labeled Z2 probes. (C) Quantitative fluorescence intensity of ESCC and adjacent tissues stained with Cy5-labeled Z4-6 probes. The asterisks indicate significance, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: To investigate the Dox loading capacity, Dox was fixed at 2 μM, and various aptamer-to-Dox ratios (0, 1:20, 1:15, 1:10, 1:7.5, 1:5, 1:3, and 1:2) were prepared as above mentioned, and Dox fluorescence was examined by F-7000 fluorescence spectrometer (Hitachi, Japan).

Techniques: Fluorescence, Imaging, Labeling, Staining, Control

Journal: Materials Today Bio

Article Title: Identification of a myosin 1B-binding aptamer for fluorescence imaging and targeted therapy of esophageal squamous cell carcinoma

doi: 10.1016/j.mtbio.2026.102867

Figure Lengend Snippet: Selective cytotoxicity of Z4-6-Dox. (A) Schematic illustration of construction of Z4-6-Dox. (B) Fluorescence spectrum of Dox incubated with various concentration of Z4-6. Dox was fixed at 2 μM. (C) Flow cytometry assays of binding of Cy5-labeled Z4-6 and Z4-6-Dox to KYSE30 cells. Confocal imaging of KYSE30 (D) and Het-1A (E) cells treated with 4 μM Dox and 0.8 μM Z4-6-Dox. Cell nuclei were stained with Hoechst33342. CCK-8 assays of KYSE30 (F) and Het-1A (G) cells treated with 0.8 μM Z4-6 and Z4-6-Dox, and 4 μM Dox. The asterisks indicate significance, ∗∗∗ P < 0.001, ns indicates no significance. (H) Calcein-AM/PI imaging of KYSE30 and Het-1A cells treated with 0.8 μM Z4-6 and Z4-6-Dox, and 4 μM Dox. The green indicates Calcein-AM-stained live cells, and the red indicates PI-stained dead cells. (I) The percentage of dead cells was calculated. Untreated cells are the controls. The asterisks indicate significance, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: To investigate the Dox loading capacity, Dox was fixed at 2 μM, and various aptamer-to-Dox ratios (0, 1:20, 1:15, 1:10, 1:7.5, 1:5, 1:3, and 1:2) were prepared as above mentioned, and Dox fluorescence was examined by F-7000 fluorescence spectrometer (Hitachi, Japan).

Techniques: Fluorescence, Incubation, Concentration Assay, Flow Cytometry, Binding Assay, Labeling, Imaging, Staining, CCK-8 Assay

Journal: Materials Today Bio

Article Title: Identification of a myosin 1B-binding aptamer for fluorescence imaging and targeted therapy of esophageal squamous cell carcinoma

doi: 10.1016/j.mtbio.2026.102867

Figure Lengend Snippet: In vivo fluorescence imaging with the Z4-6-Cy5 probe. Time-lapse fluorescence imaging of mice bearing KYSE30 tumors after injected with the Z4-6-Cy5 (A) and Random-Cy5 (C) probes. Fluorescence images of excised heart (1), liver (2), spleen (3), lung (4), kidney (5), tumor (6) tissues from mouse model injected with the Z4-6-Cy5 (B) and Random-Cy5 (D) probes. (E) The quantitative analysis of fluorescence intensity at tumor sites in mice treated with the Z4-6-Cy5 and Random-Cy5 probes. (F) The quantitative analysis of fluorescence intensity in excised tumors and major organs.

Article Snippet: To investigate the Dox loading capacity, Dox was fixed at 2 μM, and various aptamer-to-Dox ratios (0, 1:20, 1:15, 1:10, 1:7.5, 1:5, 1:3, and 1:2) were prepared as above mentioned, and Dox fluorescence was examined by F-7000 fluorescence spectrometer (Hitachi, Japan).

Techniques: In Vivo, Fluorescence, Imaging, Injection