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Fluorescence emission from the microfluidic device following 40 minutes of S. equi DNA LAMP amplification. There is equal fluorescence at 40 minutes whether LAMP detects 1 CFU or 100 CFU. CFU, colony forming unit; LAMP, loop‐mediated isothermal nucleic acid amplification

Journal: Journal of Veterinary Internal Medicine

Article Title: Detection of Streptococcus equi subsp. equi in guttural pouch lavage samples using a loop‐mediated isothermal nucleic acid amplification microfluidic device

doi: 10.1111/jvim.16105

Figure Lengend Snippet: Fluorescence emission from the microfluidic device following 40 minutes of S. equi DNA LAMP amplification. There is equal fluorescence at 40 minutes whether LAMP detects 1 CFU or 100 CFU. CFU, colony forming unit; LAMP, loop‐mediated isothermal nucleic acid amplification

Article Snippet: Logistic regression power analysis determined a sample size of 60 GPL specimens were enough for the study to demonstrate a difference between the 3 DNA amplification methods (power of 80% and α ≤ .05) (STATA 14.0).

Techniques: Fluorescence, Amplification

(A) Fluorescent by‐product of LAMP distinguished a positive vs negative result of S. equi DNA detection on dry storage microfluidic device (B) as seen via a smartphone screen. LAMP, loop‐mediated isothermal nucleic acid amplification

Journal: Journal of Veterinary Internal Medicine

Article Title: Detection of Streptococcus equi subsp. equi in guttural pouch lavage samples using a loop‐mediated isothermal nucleic acid amplification microfluidic device

doi: 10.1111/jvim.16105

Figure Lengend Snippet: (A) Fluorescent by‐product of LAMP distinguished a positive vs negative result of S. equi DNA detection on dry storage microfluidic device (B) as seen via a smartphone screen. LAMP, loop‐mediated isothermal nucleic acid amplification

Article Snippet: Logistic regression power analysis determined a sample size of 60 GPL specimens were enough for the study to demonstrate a difference between the 3 DNA amplification methods (power of 80% and α ≤ .05) (STATA 14.0).

Techniques: Amplification