Journal: Biogerontology

Article Title: Genomic and proteomic profiling of oxidative stress response in human diploid fibroblasts

doi: 10.1007/s10522-008-9157-3

Figure Lengend Snippet: Display of the proteomics data by the cytoscape network. Proteins detected only in H2O2 treated group (a, red) or control group (b, green) were imported into the Cytoscape 2.4.0 software for building the network. The nodes represent individual proteins and squares indicate gene ontology terms. The colors of the edges enhance the view of corresponding gene ontology groups in the network of microarray data

Article Snippet: Cy3 or Cy5 labeled first strand cDNAs were made from 40 μg of total RNA with Micromax Direct cDNA Microarray System (NEN Life Sciences, Boston, MA).

Techniques: Control, Software, Microarray

Journal: Biogerontology

Article Title: Genomic and proteomic profiling of oxidative stress response in human diploid fibroblasts

doi: 10.1007/s10522-008-9157-3

Figure Lengend Snippet: cDNA Microarray detection of genes changing expression in HDFs with H 2 O 2 treatment

Article Snippet: Cy3 or Cy5 labeled first strand cDNAs were made from 40 μg of total RNA with Micromax Direct cDNA Microarray System (NEN Life Sciences, Boston, MA).

Techniques: Microarray, Expressing, Functional Assay, Clinical Proteomics, Ubiquitin Proteomics, Binding Assay, Activity Assay, Coagulation, Translocation Assay, Derivative Assay, Sequencing, Phospho-proteomics, Protein Binding, Variant Assay, Virus, RNA Binding Assay

Journal: Biogerontology

Article Title: Genomic and proteomic profiling of oxidative stress response in human diploid fibroblasts

doi: 10.1007/s10522-008-9157-3

Figure Lengend Snippet: The network of genes increased or decreased in H2O2 treated cells. HDFs were treated with 600 μM H2O2 for 2 h. Microarray analyses were performed using RNA collected 72 h after H2O2 treatment. The nodes represent genes increasing (a, red) or decreasing (b, blue) in H2O2 treated cells. The color shades of nodes reflect the degree of changes, with darker color indicating more increase (red) or decrease (blue) than the lighter shade of color. The squares represent gene ontology terms. The colors of the edges enhance the view of corresponding gene ontology groups in the network of proteomic data

Article Snippet: Cy3 or Cy5 labeled first strand cDNAs were made from 40 μg of total RNA with Micromax Direct cDNA Microarray System (NEN Life Sciences, Boston, MA).

Techniques: Microarray

Journal: Biogerontology

Article Title: Genomic and proteomic profiling of oxidative stress response in human diploid fibroblasts

doi: 10.1007/s10522-008-9157-3

Figure Lengend Snippet: Display of the proteomics data by the cytoscape network. Proteins detected only in H2O2 treated group (a, red) or control group (b, green) were imported into the Cytoscape 2.4.0 software for building the network. The nodes represent individual proteins and squares indicate gene ontology terms. The colors of the edges enhance the view of corresponding gene ontology groups in the network of microarray data

Article Snippet: Cy3 or Cy5 labeled first strand cDNAs were made from 40 μg of total RNA with Micromax Direct cDNA Microarray System (NEN Life Sciences, Boston, MA).

Techniques: Software, Microarray

Journal: Biogerontology

Article Title: Genomic and proteomic profiling of oxidative stress response in human diploid fibroblasts

doi: 10.1007/s10522-008-9157-3

Figure Lengend Snippet: cDNA Microarray detection of genes changing expression in HDFs with H 2 O 2 treatment

Article Snippet: Cy3 or Cy5 labeled first strand cDNAs were made from 40 μg of total RNA with Micromax Direct cDNA Microarray System (NEN Life Sciences, Boston, MA).

Techniques: Microarray, Expressing, Functional Assay, Binding Assay, Activity Assay, Coagulation, Translocation Assay, Derivative Assay, Sequencing, Protein Binding, Variant Assay, RNA Binding Assay

Journal: Biogerontology

Article Title: Genomic and proteomic profiling of oxidative stress response in human diploid fibroblasts

doi: 10.1007/s10522-008-9157-3

Figure Lengend Snippet: The network of genes increased or decreased in H2O2 treated cells. HDFs were treated with 600 μM H2O2 for 2 h. Microarray analyses were performed using RNA collected 72 h after H2O2 treatment. The nodes represent genes increasing (a, red) or decreasing (b, blue) in H2O2 treated cells. The color shades of nodes reflect the degree of changes, with darker color indicating more increase (red) or decrease (blue) than the lighter shade of color. The squares represent gene ontology terms. The colors of the edges enhance the view of corresponding gene ontology groups in the network of proteomic data

Article Snippet: Cy3 or Cy5 labeled first strand cDNAs were made from 40 μg of total RNA with Micromax Direct cDNA Microarray System (NEN Life Sciences, Boston, MA).

Techniques: Microarray