Journal: Frontiers in microbiology

Article Title: S-layers at second glance? Altiarchaeal grappling hooks (hami) resemble archaeal S-layer proteins in structure and sequence.

doi: 10.3389/fmicb.2015.00543

Figure Lengend Snippet: FIGURE 1 | Electron micrographs of Ca. A. hamiconexum cells within the biofilm. (A) Scanning electron micrograph of dividing cells in the biofilm, showing particles close to the hami and the cells. Bar: 1 µm. (B) Thin-section of one cell, showing dark-stained areas. Bar. 200 nm.

Article Snippet: Zero loss filtered tomographic tilt series were collected in a range of −60◦ to +60◦, at increments of 2◦–2.5◦ and a defocus of 6–10μm using the Gatan Digital Micrograph Latitude software (Gatan Inc., Pleasanton, USA) The maximum cumulative dose was 150 e−/A2.

Techniques: Staining