Journal: Bioactive Materials

Article Title: Smart microenvironment-adaptive nanocatalytic hydrogel for sequential antibacterial, anti-inflammatory, and regenerative therapy of biofilm-infected wounds

doi: 10.1016/j.bioactmat.2026.02.043

Figure Lengend Snippet: Structural characterization of HC. (A) Schematic illustration of the synthesis of HC. (B, C) TEM images of Cu 5.4 O and HC. (D) Energy-dispersive X-ray spectroscopy (EDS) mapping images of C, N, Cu and O for HC. (E) Zeta potentials and hydrodynamic size distribution, and (F) XRD analysis of Cu 5.4 O, HAs and HC. (G, H) XPS spectra of Cu 2p of Cu 5.4 O and HC. (I) X-ray-induced Auger electron spectroscopy (XAES) spectra of the Cu 5.4 O. (J) Size stability of HC in different solvents (Water, PBS, FBS, DMEM) on days 3, 5, and 7 at a concentration of 200 μg/mL, with a sample size of n = 3 (mean ± SD). (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).

Article Snippet: X-ray diffraction (XRD) patterns were conducted on a Bruker D8 ADVANCE X-ray diffractometer using Cu-Kα radiation (λ = 1.5418 Å).

Techniques: Spectroscopy, Concentration Assay

Journal: International Journal of Pharmaceutics: X

Article Title: Gold-incorporated hyaluronic acid nanoparticles enhance ablative radiotherapy efficacy in lung cancer

doi: 10.1016/j.ijpx.2025.100480

Figure Lengend Snippet: Preparation and characteristics of Au-HA-functionalized SN38 NPs. (a) HA NPs are prepared by complexing SN38 with a human serum albumin (HSA)-based HA/PEI mixture. Simultaneously, Au NP-incorporated HA NPs (Au/HA NPs) are synthesized by integrating PEI-stabilized Au NPs into the HA/albumin complex. HA-functionalized SN38 NPs are prepared using a lyophilization–hydration method. (b) Transmission electron microscopy images of HA NPs and Au/HA NPs. Samples are negatively stained with 2 % uranyl acetate before imaging. Scale bar = 200 nm. (c) The average size and zeta potential of HA NPs and Au/HA NPs are analyzed using dynamic light scattering and aqueous electrophoresis. Each experimental group includes five samples ( n = 5). Data are expressed as the mean ± standard error of the mean. Statistical analyses are performed using the Mann–Whitney U test. (d) Full scan XPS spectrum of HA NPs and Au/HA NPs. (e) X-ray diffraction patterns of SN38, HA NP, and Au/HA NPs. (f) Storage stability of NPs as evaluated according to particle size (D) is determined by dissolving the re-lyophilized HA NPs and Au/HA NPs in 1 mL double-distilled water (ddH 2 O). Each experimental group include five samples ( n = 5). (g) Colloidal stability of NPs, as measured according to particle size (D), is assessed in Dulbecco's Modified Eagle Medium (Gibco) supplemented with 10 % ( v /v) fetal bovine serum to evaluate stability under physiologically relevant conditions. Each experimental group includes five samples (n = 5). (h) Representative and (i) quantitative cell surface CD44 expression posttreatment with SN38, HA NPs, and Au/HA NPs in A549, H226, and LLC cells as determined using flow cytometry. Each experimental group includes five samples (n = 5). Data are expressed as the mean ± standard error of the mean. Statistical significance is assessed using the one-way analysis of variance with Tukey's multiple comparisons test. * P < 0.05; ** P < 0.01; *** P < 0.001. Abbreviations: HA, hyaluronic acid; LLC, Lewis lung carcinoma; NP, nanoparticle; PEI, polyethyleneimine.

Article Snippet: Powder X-ray diffraction (XRD) patterns of SN38, HA NP, and Au/HA NP were recorded using a D2 PHASER X-ray diffractometer (Bruker Analytical X-Ray Solutions, Madison, WI, USA) equipped with a Cu Kα radiation source (λ = 1.5418 Å).

Techniques: Synthesized, Lyophilization, Transmission Assay, Electron Microscopy, Staining, Imaging, Zeta Potential Analyzer, Electrophoresis, MANN-WHITNEY, Modification, Expressing, Flow Cytometry