Journal: Molecular Medicine Reports

Article Title: Pathological mechanism of ferroptosis in a rat model of α-naphthyl isothiocyanate-induced chronic cholestasis

doi: 10.3892/mmr.2026.13802

Figure Lengend Snippet: Liver function of rats with chronic cholestasis is impaired and histopathological analysis of liver tissue reveals inflammation. The control group was fed a chow diet, and the ANIT and DFO-treated groups were intragastrically administered ANIT olive oil solution with or without DFO (n=8/group). (A) TBA, (B) ALP, (C) TBIL, (D) DBIL, (E) GGT, (F) LDL, (G) TCH, (H) TG, (I) ALT and (J) AST were measured. **P<0.01, ***P<0.001. (K) Hematoxylin and eosin staining (scale bar, 200 µm). Arrows indicate bile duct hyperplasia and inflammatory cell infiltration in the portal area. ANIT, α-naphthyl isothiocyanate; DFO, deferoxamine; TBA, total bile acid; ALP, alkaline phosphatase; TBIL, total bilirubin; DBIL, direct bilirubin; GGT, γ-glutamyl transferase; LDL, low-density lipoprotein; TCH, total cholesterol; TG, triglyceride; ALT, alanine aminotransferase; AST, aspartate aminotransferase.

Article Snippet: The DFO-treated group received daily DFO treatment (MedChemExpress; 100 mg/kg) ( ) for 4 weeks (weeks 9–12).

Techniques: Control, Staining

Journal: Molecular Medicine Reports

Article Title: Pathological mechanism of ferroptosis in a rat model of α-naphthyl isothiocyanate-induced chronic cholestasis

doi: 10.3892/mmr.2026.13802

Figure Lengend Snippet: Intrahepatic collagen fiber deposition is increased in rats with chronic cholestasis. Control group was fed a chow diet, whereas ANIT and DFO-treated groups were intragastrically administered ANIT olive oil solution with or without DFO (n=8/group). (A) Sirius red staining (scale bar, 200 µ m) and (B) collagen-positive area in the liver. (C) HYP levels in the liver. (D) Changes in α-SMA protein expression. The blots shown are representative of three independent experiments. The dotted line indicates that the lanes were non-adjacent on the original gel. (E) Relative expression levels of α-SMA. Results Data are presented as the mean ± SD. P-values were determined by one-way ANOVA. *P<0.05, ***P<0.001. ANIT, α-naphthyl isothiocyanate; DFO, deferoxamine; HYP, hydroxyproline; α-SMA, α-smooth muscle actin.

Article Snippet: The DFO-treated group received daily DFO treatment (MedChemExpress; 100 mg/kg) ( ) for 4 weeks (weeks 9–12).

Techniques: Control, Staining, Expressing

Journal: Molecular Medicine Reports

Article Title: Pathological mechanism of ferroptosis in a rat model of α-naphthyl isothiocyanate-induced chronic cholestasis

doi: 10.3892/mmr.2026.13802

Figure Lengend Snippet: Free iron ions are aberrantly increased in serum and liver samples from rats with chronic cholestasis. Control group was fed a chow diet, whereas ANIT and DFO-treated groups were intragastrically administered ANIT olive oil solution with or without DFO (n=8/group). (A) Serum iron, (B) liver iron and (C) liver Fe 2+ levels were detected. (D) Liver Prussian blue Fe 3+ positive area. Data are presented as the mean ± SD. P-values were determined by one-way ANOVA. *P<0.05, **P<0.01, ***P<0.001. (E) Prussian blue staining (scale bar, 200 µ m). ANIT, α-naphthyl isothiocyanate; DFO, deferoxamine.

Article Snippet: The DFO-treated group received daily DFO treatment (MedChemExpress; 100 mg/kg) ( ) for 4 weeks (weeks 9–12).

Techniques: Control, Staining

Journal: Molecular Medicine Reports

Article Title: Pathological mechanism of ferroptosis in a rat model of α-naphthyl isothiocyanate-induced chronic cholestasis

doi: 10.3892/mmr.2026.13802

Figure Lengend Snippet: Levels of lipid peroxidation and ferroptosis markers are increased in the liver of rats with chronic cholestasis and the liver ultrastructure is affected in a DFO-reversible manner. Control group was fed a chow diet, whereas ANIT and DFO-treated groups were intragastrically administered ANIT olive oil solution with or without DFO (n=8/group). Levels of (A) MDA, (B) GSH and (C) GSH-PX were detected. (D) Changes in ferroptosis marker protein expression. The blots shown are representative of three independent experiments. A dotted line indicates that the lanes were non-adjacent on the original gel. All target protein bands and their corresponding loading control bands shown side-by-side were derived from the same membrane. Relative expression levels of (E) ASCL4 and (F) COX2. Data are presented as the mean ± SD. P-values were determined by one-way ANOVA. *P<0.05, **P<0.01, ***P<0.001. (G) Transmission electron microscope sections, blue arrows indicate mitochondrial cristae, yellow arrows indicate outer mitochondrial membrane and green arrows point to mitochondria (scale bars, 5 and 2 µ m). DFO, deferoxamine; ANIT, α-naphthyl isothiocyanate; MDA, malondialdehyde; GSH, glutathione; GSH-PX, glutathione peroxidase; ASCL4, acyl-CoA synthetase long-chain family member 4; COX2, cyclooxygenase 2.

Article Snippet: The DFO-treated group received daily DFO treatment (MedChemExpress; 100 mg/kg) ( ) for 4 weeks (weeks 9–12).

Techniques: Control, Marker, Expressing, Derivative Assay, Membrane, Transmission Assay, Microscopy

Journal: Molecular Medicine Reports

Article Title: Pathological mechanism of ferroptosis in a rat model of α-naphthyl isothiocyanate-induced chronic cholestasis

doi: 10.3892/mmr.2026.13802

Figure Lengend Snippet: Abnormal expression of antioxidant and iron metabolism-related proteins in liver samples of rats with chronic cholestasis. Control group was fed a chow diet, whereas the ANIT and DFO-treated groups were intragastrically administered ANIT olive oil solution with or without DFO (n=8/group). (A) Changes in ferroptosis antioxidant-related protein expression. The blots shown are representative of three independent experiments. A dotted line indicates that the lanes were non-adjacent on the original gel. All target protein bands and their corresponding loading control bands shown side-by-side were derived from the same membrane. Relative expression levels of (B) Nrf2, (C) Keap1, (D) XCT, (E) HO-1 and (F) GPX4. (G) Changes in iron metabolism-related protein expression. The blots shown are representative of three independent experiments. A dotted line indicates that the lanes were non-adjacent on the original gel. All target protein bands and their corresponding loading control bands shown side-by-side were derived from the same membrane. Relative expression levels of (H) TFR1, (I) FPN1, (J) DMT1, (K) Steap3 and (L) FTH1. Data are presented as the mean ± SD. P-values were determined by one-way ANOVA. *P<0.05, **P<0.01, ***P<0.001. ANIT, α-naphthyl isothiocyanate; DFO, deferoxamine; Nrf2, nuclear factor erythroid-2-related factor 2; Keap1, Kelch-like ECH-associated protein 1, XCT, cystine/glutamate transporter; HO-1, heme oxygenase 1; GPX4, glutathione peroxidase 4; TFR1, transferrin receptor 1; FPN1, ferroportin 1; DMT1, divalent metal transporter 1; Steap3, six-transmembrane epithelial antigen of the prostate 3; FTH1, ferritin heavy chain 1.

Article Snippet: The DFO-treated group received daily DFO treatment (MedChemExpress; 100 mg/kg) ( ) for 4 weeks (weeks 9–12).

Techniques: Expressing, Control, Derivative Assay, Membrane