Journal: bioRxiv

Article Title: Reduced enteric BDNF-TrkB signaling drives glucocorticoid-mediated GI dysmotility

doi: 10.1101/2024.12.13.628260

Figure Lengend Snippet: Graphical representation of the mean ± standard error of the whole gut transit times (assayed using the carmine-red dye method) of two cohorts of mice, where the mice in the ‘Dexa’ cohort were dosed once with dexamethasone (5mg/Kg body weight), and the ‘Control’ cohort mice were dosed with Vehicle (10% DMSO in Saline). Mice in the Dexa cohort showed significantly longer whole gut transit times on average than mice in the control cohort. *p < 0.05. Student’s t-test.

Article Snippet: Mice in the control group were treated with vehicle (10% DMSO in saline, intraperitoneal route) and those in the DEXA group were treated with dexamethasone (MedChemExpress, Cat. No. HY-14648; 5mg/kg in 10% DMSO/body weight, intraperitoneal route) group.

Techniques: Control, Saline

Journal: bioRxiv

Article Title: Reduced enteric BDNF-TrkB signaling drives glucocorticoid-mediated GI dysmotility

doi: 10.1101/2024.12.13.628260

Figure Lengend Snippet: (A) Adult male mice dosed with dexamethasone (Dexa) and sacrificed 4 hours later showed a significant down regulation in the expression of stress and glucocorticoid-responsive Bdnf IV and Bdnf VI transcripts in their small intestinal LM-MP tissues, when compared to age- and sex-matched mice that were dosed with vehicle. *p < 0.05, **p < 0.01, Students’ t-test. (B) Small intestinal LM-MP tissues from adult male mice that were cultured with Dexa for 4 hours showed a significant down regulation in the expression of stress and glucocorticoid-responsive Bdnf IV and Bdnf VI transcripts in their small intestinal LM-MP tissues, when compared to tissues from age- and sex-matched mice that were cultured with vehicle. *p < 0.05, Students’ t-test. (C) Small intestinal LM-MP tissues from adult male mice that were cultured with Dexa for 4 hours showed a significant reduction in the abundance of BDNF protein as assessed by ELISA, when compared to tissues from age- and sex-matched mice that were cultured with vehicle. **p < 0.01, Students’ t-test. (D) Dexa-treated adult male mice that were also dosed with the synthetic and specific agonist of TrkB HIOS showed significant reduction in their whole gut transit time of orally gavaged carmine-red dye, and hence improved gut motility, when compared to age- and sex-matched mice that were dosed only with Dexa.

Article Snippet: Mice in the control group were treated with vehicle (10% DMSO in saline, intraperitoneal route) and those in the DEXA group were treated with dexamethasone (MedChemExpress, Cat. No. HY-14648; 5mg/kg in 10% DMSO/body weight, intraperitoneal route) group.

Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay