Journal: Biosensors

Article Title: Performance of Colorimetric Lateral Flow Immunoassays for Renal Function Evaluation with Human Serum Cystatin C

doi: 10.3390/bios15070445

Figure Lengend Snippet: The 1 μL sample volume with 5 μL AuNPs’ conjugated mAbs and 54 μL running buffer calibration curves in ( a ) OD values’ optimisation in regard to OD 10, OD 5, OD 2.5; ( b ) calibration curves with CN 95, CN 110, and CN140 (OD 5 AuNPs).

Article Snippet: A λ is the absorbance at the wavelength λ [ ]. (1) O D λ = A λ L = 1 L log 10 I 0 I In total, 40 OD AuNPs (40 nm, ab269942, Abcam, Cambridge, UK) were conjugated with the 89.5 μg/mL detector mAbs (Fitzgerald, 10-7887, Stratech, Ely, UK).

Techniques:

Journal: bioRxiv

Article Title: The hyper-attenuated RVFV 40Fp8 strain can be safely administered to pregnant ewes to protect them from a virulent challenge

doi: 10.1101/2025.01.22.634310

Figure Lengend Snippet: A. Evolution of rectal temperature in ewes after SC (light blue) or IV (dark blue) inoculation with 40Fp8 virus. The horizontal black dotted line marks hyperthermia. B. Serological determination of neutralizing antibody titers by TCID50 microneutralization assay (left panel) and competition ELISA anti-N (right panel). Both graphs show mean plus SD values. The dotted line depicts the sensitivity threshold (1/10 dilution). The grey shaded area depicts the doubtful range. C. Detection of IFNγ in plasma by capture ELISA. Blood samples taken at 14 or 21 days after SC or IV 40Fp8 inoculation were re-stimulated with recombinant RVFV Gn or N proteins or peptides derived from the Gn (#19 and #21) or Gc (#226 and #253) protein sequences. Each symbol denotes individual values (after subtraction of values derived from unstimulated plasma) and horizontal bars denote mean values.

Article Snippet: Upon incubation and washing steps a detector anti-bovine IFNγ mAb conjugated with biotin (MT307, Mabtech) was added.

Techniques: Virus, Microneutralization Assay, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Recombinant, Derivative Assay