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densitometry quantification  (Bio-Rad)


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    Structured Review

    Bio-Rad densitometry quantification
    Densitometry Quantification, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 36323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/densitometry+quantification+software/Image+Lab+Software/pm41397959-607-0-7
    Average 99 stars, based on 36323 article reviews
    densitometry quantification - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Software:

    Article Title: The double-hit protocol induces HFpEF and impairs myocardial ubiquitin-proteasome system performance in FVB/N mice
    Article Snippet: The bound secondary antibodies on the PVDF were then detected with the SuperSignal West Pico PLUS Chemiluminescent Substrates (ThermoScientific) and imaged with a ChemiDoc MP imaging system (Bio-Rad). .. Densitometry quantification was performed using the ImageLab software (Bio-Rad). .. RNA isolation, reverse transcription, and real time PCR (qPCR) Total RNA was extracted from left ventricular myocardium using TRI Reagent® (Molecular Research Center Inc., Cincinnati, OH).

    Article Title: Healthspan improvement and anti-aggregation effects induced by a marine-derived structural proteasome activator.
    Article Snippet: .. Densitometry analysis for the quantification of blots was performed with Bio-Rad’s Image Lab software 6.0.1 or ImageJ 1.52a (Bio-Rad Laboratories, Hercules, USA). ..

    Article Title: Comparative analysis of oral and intravenous iron therapy in rat models of inflammatory anemia and iron deficiency
    Article Snippet: The membrane was incubated either with human anti-TfR1-antibody (0.5 μg/ml, Zymed, Vienna, Austria), human anti-ferritin-antibody (2 μg/ml, Dako, Vienna, Austria), rabbit anti-rat-ferroportin-antibody, rabbit anti-NCOA4 antibody (1mg/ml, Abcam, Austria) or human anti-actin (2 μg/ml, Sigma, Germany) and further processed as described. (13) Actin hybridization was used to demonstrate equal protein loading onto gels. .. For quantification, densitometry data were acquired on a ChemiDoc Touch Imaging System (Bio-Rad) and analyzed with Quantity One software (Bio-Rad). ..

    Article Title: Histone methyltransferase PRDM9 promotes survival of drug-tolerant persister cells in glioblastoma
    Article Snippet: Detection was performed with Immobilon Western HRP Substrate Luminol-Peroxidase kit (MerckMillipore, Cat # WBKLS0500) and the ChemiDoc MP Imaging System (Bio-Rad). .. Densitometry quantification was done in ImageLab software (Bio-Rad). .. The following antibodies were purchased from Cell Signalling Technologies: GAPDH (1:2000, Cat# 97166), H3K4me1 (1:1000, Cat# 5326), H3K4me2 (1:1000, Cat# 9725), H3K4me3 (1:1000, Cat# 9751S), H3K27me1 (1:1000, Cat# 84932), H3K27me3 (1:1000, Cat# 9733), Histone H3 (1:2000, Cat# 4499), Cleaved Histone H3 (Thr22) (1:1000, Cat# 12576), DHCR24 (1:1000, Cat# 2033), HMGCS1 (1:1000, Cat# 36877S), SETD1B (1:1000, Cat# 44922S), Rabbit IgG HRP-linked (1:2000, Cat# 7074) and Mouse IgG HRP-linked (1:2000, Cat# 7076).

    Article Title: Histone methyltransferase PRDM9 promotes survival of drug-tolerant persister cells in glioblastoma.
    Article Snippet: Detection was performed with Immobilon Western HRP Substrate LuminolPeroxidase kit (MerckMillipore, Cat # WBKLS0500) and the ChemiDoc MP Imaging System (Bio-Rad). .. Densitometry quantification was done in ImageLab software (Bio-Rad). .. The following antibodies were purchased from Cell Signalling Technologies: GAPDH (1:2000, Cat# 97166), H3K4me1 (1:1000, Cat# 5326), H3K4me2 (1:1000, Cat# 9725), H3K4me3 (1:1000, Cat# 9751S), H3K27me1 (1:1000, Cat# 84932), H3K27me3 (1:1000, Cat# 9733), Histone H3 (1:2000, Cat# 4499), Cleaved Histone H3 (Thr22) (1:1000, Cat# 12576), DHCR24 (1:1000, Cat# 2033), HMGCS1 (1:1000, Cat# 36877S), SETD1B (1:1000, Cat# 44922S), Rabbit IgG HRP-linked (1:2000, Cat# 7074) and Mouse IgG HRP-linked (1:2000, Cat# 7076).

    Article Title: Shoc2 Deficiency Disrupts Lymphangiogenesis through mTOR-Mediated Mitochondrial Dysfunction
    Article Snippet: .. Quantification was performed using the densitometry analysis mode of Image Lab software (Bio-Rad, Hercules, CA, USA). ..

    Imaging:

    Article Title: Comparative analysis of oral and intravenous iron therapy in rat models of inflammatory anemia and iron deficiency
    Article Snippet: The membrane was incubated either with human anti-TfR1-antibody (0.5 μg/ml, Zymed, Vienna, Austria), human anti-ferritin-antibody (2 μg/ml, Dako, Vienna, Austria), rabbit anti-rat-ferroportin-antibody, rabbit anti-NCOA4 antibody (1mg/ml, Abcam, Austria) or human anti-actin (2 μg/ml, Sigma, Germany) and further processed as described. (13) Actin hybridization was used to demonstrate equal protein loading onto gels. .. For quantification, densitometry data were acquired on a ChemiDoc Touch Imaging System (Bio-Rad) and analyzed with Quantity One software (Bio-Rad). ..

    other:

    Article Title: The ubiquitin-like modifier FAT10 covalently modifies HUWE1 and strengthens the interaction of AMBRA1 and HUWE1.
    Article Snippet: For quantification ECL signals were analysed with densitometry calculations (ImageLab Software, BioRad, Basel, Switzerland) and the values normalized to the respective proteins in the lysate or to the loading control β-actin.

    Article Title: The ubiquitin-like modifier FAT10 covalently modifies HUWE1 and strengthens the interaction of AMBRA1 and HUWE1
    Article Snippet: For quantification ECL signals were analysed with densitometry calculations (ImageLab Software, BioRad, Basel, Switzerland) and the values normalized to the respective proteins in the lysate or to the loading control β-actin.



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    A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by <t>densitometry</t> and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).
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    A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by <t>densitometry</t> and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).
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    A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by <t>densitometry</t> and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).
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    Image Search Results


    A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).

    Journal: PLoS ONE

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    doi: 10.1371/journal.pone.0163958

    Figure Lengend Snippet: A, B : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from five male healthy donors, (H1 to H5) (A), and five females (H6 to H10) (B) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration was evaluated by the displayed linear regression. C, D : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (C) and HK cleavage (D), measured as in panel A. densito: densitometry in 10 7 arbitrary units; NS: non-significant; ****: p <10 −4 (Mann-Whitney-Wilcoxon test).

    Article Snippet: Using enhanced chemiluminescence (Clarity Western ECL TM substrate, Biorad, Hercules, USA) and densitometry scan quantification (ChemiDoc TM XRS+ System camera and Image Lab TM software, Bio-Rad, Hercules, USA), three molecular species were retained for data processing: native protein (N-HK, 120kDa), light chain (LC-HK, 56kDa), and cleaved light chain (cLC-HK, 46kDa).

    Techniques: Purification, Clinical Proteomics, SDS Page, Concentration Assay, MANN-WHITNEY

    A : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from nC1Inh-AE male patients (M1 to M5) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration by the displayed linear regression. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (B) and HK cleavage (C), measured as in panel A, compared to the healthy cohort studied in . D, E, F : as in A, B, C, concerning female patients (W1 to W5). densito: densitometry in 10 7 arbitrary units; ****: p <10 −4 (Mann-Whitney-Wilcoxon test); nC1Inh-AE: angioedema with normal C1 inhibitor.

    Journal: PLoS ONE

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    doi: 10.1371/journal.pone.0163958

    Figure Lengend Snippet: A : Purified human HK (20, 40, 80, and 110ng) and 0.5μL of plasma from nC1Inh-AE male patients (M1 to M5) were subjected to SDS-PAGE. The signal of HK native chain (N-HK), light chain (LC-HK), and cleaved light chain (cLC-HK) were quantified by densitometry and N-HK concentration by the displayed linear regression. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of plasma concentration of N-HK (B) and HK cleavage (C), measured as in panel A, compared to the healthy cohort studied in . D, E, F : as in A, B, C, concerning female patients (W1 to W5). densito: densitometry in 10 7 arbitrary units; ****: p <10 −4 (Mann-Whitney-Wilcoxon test); nC1Inh-AE: angioedema with normal C1 inhibitor.

    Article Snippet: Using enhanced chemiluminescence (Clarity Western ECL TM substrate, Biorad, Hercules, USA) and densitometry scan quantification (ChemiDoc TM XRS+ System camera and Image Lab TM software, Bio-Rad, Hercules, USA), three molecular species were retained for data processing: native protein (N-HK, 120kDa), light chain (LC-HK, 56kDa), and cleaved light chain (cLC-HK, 46kDa).

    Techniques: Purification, Clinical Proteomics, SDS Page, Concentration Assay, MANN-WHITNEY

    High-molecular-weight kininogen (HK) molecular pattern of plasma samples from healthy women taking or not estrogen/progestin combination (OP; Healthy+OP, n = 32; Healthy-OP, n = 30) and from nC1Inh-AE patients taking OP and ≥2 months after OP withdrawal (nC1INh-AE+/-OP, n = 13). A : Analysis of plasma samples (0.5μL/lane) from six patients (O1–O6) and the corresponding figures. N-HK concentration of P1 and P2 was evaluated by the displayed linear regression, kinin-forming activity was determined as in , pathological values were labelled in bold fonts. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of the plasma concentration of N-HK (B) and cleaved HK species (C), measured as in panel A. D, E : Details of the paired samples of panel B and C respectively analysed using the Wilcoxon matched-pairs signed rank test. densito: densitometry in 10 7 arbitrary units; NS : non-significant; *: p <0.05; **: p <0.01; ***: p <0.001; ****: p <10 −4 (non-parametric tests); N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively; nC1Inh-AE: angioedema with normal C1 inhibitor.

    Journal: PLoS ONE

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    doi: 10.1371/journal.pone.0163958

    Figure Lengend Snippet: High-molecular-weight kininogen (HK) molecular pattern of plasma samples from healthy women taking or not estrogen/progestin combination (OP; Healthy+OP, n = 32; Healthy-OP, n = 30) and from nC1Inh-AE patients taking OP and ≥2 months after OP withdrawal (nC1INh-AE+/-OP, n = 13). A : Analysis of plasma samples (0.5μL/lane) from six patients (O1–O6) and the corresponding figures. N-HK concentration of P1 and P2 was evaluated by the displayed linear regression, kinin-forming activity was determined as in , pathological values were labelled in bold fonts. B, C : Box-plot displaying 5–95% range ( italic fonts), median ( bold fonts), and interquartile values of the plasma concentration of N-HK (B) and cleaved HK species (C), measured as in panel A. D, E : Details of the paired samples of panel B and C respectively analysed using the Wilcoxon matched-pairs signed rank test. densito: densitometry in 10 7 arbitrary units; NS : non-significant; *: p <0.05; **: p <0.01; ***: p <0.001; ****: p <10 −4 (non-parametric tests); N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively; nC1Inh-AE: angioedema with normal C1 inhibitor.

    Article Snippet: Using enhanced chemiluminescence (Clarity Western ECL TM substrate, Biorad, Hercules, USA) and densitometry scan quantification (ChemiDoc TM XRS+ System camera and Image Lab TM software, Bio-Rad, Hercules, USA), three molecular species were retained for data processing: native protein (N-HK, 120kDa), light chain (LC-HK, 56kDa), and cleaved light chain (cLC-HK, 46kDa).

    Techniques: High Molecular Weight, Clinical Proteomics, Concentration Assay, Activity Assay

    A : High-molecular-weight kininogen (HK) molecular pattern of plasma samples (0.5μL/lane) from two nC1Inh-AE patients (W6, M6) less than 24h after the onset of an AE attack (Att) and at least two weeks after remission (Rem), analysed as in . B : Same as for A, with plasma samples from three patients (K1–K3) with ACEi-AE and low kinin catabolism (L-Cat) and three patients (M7–M9) with nC1Inh-AE and normal kinin catabolism (N-Cat). N-HK concentrations were evaluated by the displayed linear regressions, kinin-forming activity was determined as in , pathological values were labelled in bold font. densito: densitometry in 10 7 arbitrary units; N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively.

    Journal: PLoS ONE

    Article Title: Kininogen Cleavage Assay: Diagnostic Assistance for Kinin-Mediated Angioedema Conditions

    doi: 10.1371/journal.pone.0163958

    Figure Lengend Snippet: A : High-molecular-weight kininogen (HK) molecular pattern of plasma samples (0.5μL/lane) from two nC1Inh-AE patients (W6, M6) less than 24h after the onset of an AE attack (Att) and at least two weeks after remission (Rem), analysed as in . B : Same as for A, with plasma samples from three patients (K1–K3) with ACEi-AE and low kinin catabolism (L-Cat) and three patients (M7–M9) with nC1Inh-AE and normal kinin catabolism (N-Cat). N-HK concentrations were evaluated by the displayed linear regressions, kinin-forming activity was determined as in , pathological values were labelled in bold font. densito: densitometry in 10 7 arbitrary units; N-HK, LC-HK, cLC-HK: native chain, light chain, cleaved light chain of HK respectively.

    Article Snippet: Using enhanced chemiluminescence (Clarity Western ECL TM substrate, Biorad, Hercules, USA) and densitometry scan quantification (ChemiDoc TM XRS+ System camera and Image Lab TM software, Bio-Rad, Hercules, USA), three molecular species were retained for data processing: native protein (N-HK, 120kDa), light chain (LC-HK, 56kDa), and cleaved light chain (cLC-HK, 46kDa).

    Techniques: High Molecular Weight, Clinical Proteomics, Activity Assay