Journal: bioRxiv

Article Title: SAMHD1 Knockout iPSC model enables high lenti-viral transduction in myeloid cell types

doi: 10.1101/2025.02.04.636295

Figure Lengend Snippet: a. Schematic illustration of both iMacrophage induction protocols. Upper, iPSCs cultured in mTeSR1 media were induced into iHPC first with addition of media A and media B before induced into iMacrophage (iMac ST) with media IMDM with FBS, supplemented with MCSF, GMCSF and IL-3; lower, E8 Flex maintained iPSCs were cultured in E8 Flex media supplemented with BMP4, VEGF,SCF in 384 well round bottom plate for 4 days before induced into immature macrophage with StemPro34, supplemented with IL-3, MCSF,Glutamax, β-mercaptoethanol; the iMac Pre(cursors) were then transferred to 6 well plate into iMacrophage (iMac) with RPMI, FBS, Glutamax, MCSF, b. RNA sequencing data cluster analysis indicates duplicated samples in each stage express specific marker genes, c. Principle Component Analysis indicate iPSCs clusters together, away from iHPC, and iMac of both induction cluster together, as well as iMac precursors, d. Schematic illustration of sampling iPSC with barcodes across both inductions, e. iPSC, iMac and iMac ST three populations of the individual barcode counts.

Article Snippet: 300k/well macrophage of Wild Type and SAMHD1 KO were cultured with Media C [500 mL IMDM (Gibco), 10% defined FBS (Gibco), 5 mL Penicillin/Streptomycin (Gibco)), 20 ng/mL of hIL3 (Peprotech), 20 ng/mL of hGMCSF (Peprotech) and 20 ng/mL of hM-CSF (Peprotech)].

Techniques: Cell Culture, RNA Sequencing Assay, Marker, Sampling

Journal: STAR Protocols

Article Title: Protocol for assessing immune-target cell interactions using a single-cell cytotoxicity assay

doi: 10.1016/j.xpro.2024.103558

Figure Lengend Snippet:

Article Snippet: Fetal bovine serum, defined (FBS) , Gibco , Cat# A5256701.

Techniques: Isolation, Recombinant, Saline, Software