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Tcrα And Tcrβ Repertoire Deep Sequencing Data, supplied by Adaptive Biotechnologies Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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illumina-based deep sequencing data  (Illumina Inc)
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Illumina Inc illumina-based deep sequencing data
(A) Cryo-EM density map of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 spike trimer. Spike chains (S chain A, B or C) are colored either gray, cyan, or green. PRO-37587 Fab heavy (HC) and light (LC) chains are colored brown and beige, respectively. (B) Molecular model of PRO-37587 Fab bound to a single SARS-CoV-2 Omicron BA.1 RBD. PRO-37587 Fab backbone is depicted as cartoon with colors as shown in (A). SARS-CoV-2 RBD is depicted with a surface representation, with contacts colored red, non-contacts colored cyan, and glycans colored orange. Contacting residues on the RBD are highlighted. (C) Comparison of contacting residues for PRO-37587 Fab (PDB: 9NFT, left) and reference molecule S2X259 Fab (PDB: 7RAL, right). Backbone is depicted as a transparent cartoon and contacts on the Fabs are shown as sticks. Mutational differences between PRO-37587 and S2X259 Fabs that make contacts at the interface are highlighted. SARS-CoV-2 RBD is colored as in (B). (D) SARS-CoV-2 Omicron BA.1 RBD contacts (left) contrasted against <t>sequence</t> conservation (right). Contacts are highlighted with text and are colored as in (B). Sequence conservation is measured as the positional sequence entropy (in bits) of 18 different SARS-CoV-2 variants, including Omicron lineages (SARS-CoV-2 WIV04, D614G, Delta, Omicron BA.1, BA.2, BA.4, BA.5, JN.1, XBB.1.1, LB.1, XBB.1.5, EG.5.1, BQ.1.1, KP.2, XBB.1.16.1, BA.2.86, KP.3 and KP.3.1.1). A higher entropy (red) indicates a larger variation of mutations at that position, and a lower entropy (cyan) indicates less variation. (E) Sequence population of SARS-CoV-2 RBD contacting residues, as measured across the variants used in (D). (F) From left to right, model of SARS-CoV-2 Omicron BA.1 spike with PRO-37587 Fabs bound to all three RBDs, a close up of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 RBD with an overlay of SARS-CoV-2 pre-Omicron RBD (PDB: 7RAL), and a close-up of the altered helix conformation observed in our structure and previously reported. Molecules are colored as in (B), with SARS-CoV-2 pre-Omicron RBD reference colored orange.
Illumina Based Deep Sequencing Data, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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illumina-based deep sequencing data - by Bioz Stars, 2026-04
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workflow for processing illumina deep sequencing chip-seq data  (Illumina Inc)
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Illumina Inc workflow for processing illumina deep sequencing chip-seq data
(A) Cryo-EM density map of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 spike trimer. Spike chains (S chain A, B or C) are colored either gray, cyan, or green. PRO-37587 Fab heavy (HC) and light (LC) chains are colored brown and beige, respectively. (B) Molecular model of PRO-37587 Fab bound to a single SARS-CoV-2 Omicron BA.1 RBD. PRO-37587 Fab backbone is depicted as cartoon with colors as shown in (A). SARS-CoV-2 RBD is depicted with a surface representation, with contacts colored red, non-contacts colored cyan, and glycans colored orange. Contacting residues on the RBD are highlighted. (C) Comparison of contacting residues for PRO-37587 Fab (PDB: 9NFT, left) and reference molecule S2X259 Fab (PDB: 7RAL, right). Backbone is depicted as a transparent cartoon and contacts on the Fabs are shown as sticks. Mutational differences between PRO-37587 and S2X259 Fabs that make contacts at the interface are highlighted. SARS-CoV-2 RBD is colored as in (B). (D) SARS-CoV-2 Omicron BA.1 RBD contacts (left) contrasted against <t>sequence</t> conservation (right). Contacts are highlighted with text and are colored as in (B). Sequence conservation is measured as the positional sequence entropy (in bits) of 18 different SARS-CoV-2 variants, including Omicron lineages (SARS-CoV-2 WIV04, D614G, Delta, Omicron BA.1, BA.2, BA.4, BA.5, JN.1, XBB.1.1, LB.1, XBB.1.5, EG.5.1, BQ.1.1, KP.2, XBB.1.16.1, BA.2.86, KP.3 and KP.3.1.1). A higher entropy (red) indicates a larger variation of mutations at that position, and a lower entropy (cyan) indicates less variation. (E) Sequence population of SARS-CoV-2 RBD contacting residues, as measured across the variants used in (D). (F) From left to right, model of SARS-CoV-2 Omicron BA.1 spike with PRO-37587 Fabs bound to all three RBDs, a close up of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 RBD with an overlay of SARS-CoV-2 pre-Omicron RBD (PDB: 7RAL), and a close-up of the altered helix conformation observed in our structure and previously reported. Molecules are colored as in (B), with SARS-CoV-2 pre-Omicron RBD reference colored orange.
Workflow For Processing Illumina Deep Sequencing Chip Seq Data, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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workflow for processing illumina deep sequencing chip-seq data - by Bioz Stars, 2026-04
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deep rna-sequencing data  (Illumina Inc)
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Illumina Inc deep rna-sequencing data
(A) Cryo-EM density map of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 spike trimer. Spike chains (S chain A, B or C) are colored either gray, cyan, or green. PRO-37587 Fab heavy (HC) and light (LC) chains are colored brown and beige, respectively. (B) Molecular model of PRO-37587 Fab bound to a single SARS-CoV-2 Omicron BA.1 RBD. PRO-37587 Fab backbone is depicted as cartoon with colors as shown in (A). SARS-CoV-2 RBD is depicted with a surface representation, with contacts colored red, non-contacts colored cyan, and glycans colored orange. Contacting residues on the RBD are highlighted. (C) Comparison of contacting residues for PRO-37587 Fab (PDB: 9NFT, left) and reference molecule S2X259 Fab (PDB: 7RAL, right). Backbone is depicted as a transparent cartoon and contacts on the Fabs are shown as sticks. Mutational differences between PRO-37587 and S2X259 Fabs that make contacts at the interface are highlighted. SARS-CoV-2 RBD is colored as in (B). (D) SARS-CoV-2 Omicron BA.1 RBD contacts (left) contrasted against <t>sequence</t> conservation (right). Contacts are highlighted with text and are colored as in (B). Sequence conservation is measured as the positional sequence entropy (in bits) of 18 different SARS-CoV-2 variants, including Omicron lineages (SARS-CoV-2 WIV04, D614G, Delta, Omicron BA.1, BA.2, BA.4, BA.5, JN.1, XBB.1.1, LB.1, XBB.1.5, EG.5.1, BQ.1.1, KP.2, XBB.1.16.1, BA.2.86, KP.3 and KP.3.1.1). A higher entropy (red) indicates a larger variation of mutations at that position, and a lower entropy (cyan) indicates less variation. (E) Sequence population of SARS-CoV-2 RBD contacting residues, as measured across the variants used in (D). (F) From left to right, model of SARS-CoV-2 Omicron BA.1 spike with PRO-37587 Fabs bound to all three RBDs, a close up of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 RBD with an overlay of SARS-CoV-2 pre-Omicron RBD (PDB: 7RAL), and a close-up of the altered helix conformation observed in our structure and previously reported. Molecules are colored as in (B), with SARS-CoV-2 pre-Omicron RBD reference colored orange.
Deep Rna Sequencing Data, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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deep rna sequencing data  (GeneLAB GmbH)
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(A) Cryo-EM density map of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 spike trimer. Spike chains (S chain A, B or C) are colored either gray, cyan, or green. PRO-37587 Fab heavy (HC) and light (LC) chains are colored brown and beige, respectively. (B) Molecular model of PRO-37587 Fab bound to a single SARS-CoV-2 Omicron BA.1 RBD. PRO-37587 Fab backbone is depicted as cartoon with colors as shown in (A). SARS-CoV-2 RBD is depicted with a surface representation, with contacts colored red, non-contacts colored cyan, and glycans colored orange. Contacting residues on the RBD are highlighted. (C) Comparison of contacting residues for PRO-37587 Fab (PDB: 9NFT, left) and reference molecule S2X259 Fab (PDB: 7RAL, right). Backbone is depicted as a transparent cartoon and contacts on the Fabs are shown as sticks. Mutational differences between PRO-37587 and S2X259 Fabs that make contacts at the interface are highlighted. SARS-CoV-2 RBD is colored as in (B). (D) SARS-CoV-2 Omicron BA.1 RBD contacts (left) contrasted against <t>sequence</t> conservation (right). Contacts are highlighted with text and are colored as in (B). Sequence conservation is measured as the positional sequence entropy (in bits) of 18 different SARS-CoV-2 variants, including Omicron lineages (SARS-CoV-2 WIV04, D614G, Delta, Omicron BA.1, BA.2, BA.4, BA.5, JN.1, XBB.1.1, LB.1, XBB.1.5, EG.5.1, BQ.1.1, KP.2, XBB.1.16.1, BA.2.86, KP.3 and KP.3.1.1). A higher entropy (red) indicates a larger variation of mutations at that position, and a lower entropy (cyan) indicates less variation. (E) Sequence population of SARS-CoV-2 RBD contacting residues, as measured across the variants used in (D). (F) From left to right, model of SARS-CoV-2 Omicron BA.1 spike with PRO-37587 Fabs bound to all three RBDs, a close up of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 RBD with an overlay of SARS-CoV-2 pre-Omicron RBD (PDB: 7RAL), and a close-up of the altered helix conformation observed in our structure and previously reported. Molecules are colored as in (B), with SARS-CoV-2 pre-Omicron RBD reference colored orange.
Deep Rna Sequencing Data, supplied by GeneLAB GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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deep rna sequencing data - by Bioz Stars, 2026-04
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hiseq x10 sequencing platform whole-genome second-generation deep sequencing data (>30)  (Illumina Inc)
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Illumina Inc hiseq x10 sequencing platform whole-genome second-generation deep sequencing data (>30)
(A) Cryo-EM density map of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 spike trimer. Spike chains (S chain A, B or C) are colored either gray, cyan, or green. PRO-37587 Fab heavy (HC) and light (LC) chains are colored brown and beige, respectively. (B) Molecular model of PRO-37587 Fab bound to a single SARS-CoV-2 Omicron BA.1 RBD. PRO-37587 Fab backbone is depicted as cartoon with colors as shown in (A). SARS-CoV-2 RBD is depicted with a surface representation, with contacts colored red, non-contacts colored cyan, and glycans colored orange. Contacting residues on the RBD are highlighted. (C) Comparison of contacting residues for PRO-37587 Fab (PDB: 9NFT, left) and reference molecule S2X259 Fab (PDB: 7RAL, right). Backbone is depicted as a transparent cartoon and contacts on the Fabs are shown as sticks. Mutational differences between PRO-37587 and S2X259 Fabs that make contacts at the interface are highlighted. SARS-CoV-2 RBD is colored as in (B). (D) SARS-CoV-2 Omicron BA.1 RBD contacts (left) contrasted against <t>sequence</t> conservation (right). Contacts are highlighted with text and are colored as in (B). Sequence conservation is measured as the positional sequence entropy (in bits) of 18 different SARS-CoV-2 variants, including Omicron lineages (SARS-CoV-2 WIV04, D614G, Delta, Omicron BA.1, BA.2, BA.4, BA.5, JN.1, XBB.1.1, LB.1, XBB.1.5, EG.5.1, BQ.1.1, KP.2, XBB.1.16.1, BA.2.86, KP.3 and KP.3.1.1). A higher entropy (red) indicates a larger variation of mutations at that position, and a lower entropy (cyan) indicates less variation. (E) Sequence population of SARS-CoV-2 RBD contacting residues, as measured across the variants used in (D). (F) From left to right, model of SARS-CoV-2 Omicron BA.1 spike with PRO-37587 Fabs bound to all three RBDs, a close up of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 RBD with an overlay of SARS-CoV-2 pre-Omicron RBD (PDB: 7RAL), and a close-up of the altered helix conformation observed in our structure and previously reported. Molecules are colored as in (B), with SARS-CoV-2 pre-Omicron RBD reference colored orange.
Hiseq X10 Sequencing Platform Whole Genome Second Generation Deep Sequencing Data (>30), supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hiseq x10 sequencing platform whole-genome second-generation deep sequencing data (>30) - by Bioz Stars, 2026-04
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deep sequencing data  (Illumina Inc)
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Illumina Inc deep sequencing data
(A) Cryo-EM density map of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 spike trimer. Spike chains (S chain A, B or C) are colored either gray, cyan, or green. PRO-37587 Fab heavy (HC) and light (LC) chains are colored brown and beige, respectively. (B) Molecular model of PRO-37587 Fab bound to a single SARS-CoV-2 Omicron BA.1 RBD. PRO-37587 Fab backbone is depicted as cartoon with colors as shown in (A). SARS-CoV-2 RBD is depicted with a surface representation, with contacts colored red, non-contacts colored cyan, and glycans colored orange. Contacting residues on the RBD are highlighted. (C) Comparison of contacting residues for PRO-37587 Fab (PDB: 9NFT, left) and reference molecule S2X259 Fab (PDB: 7RAL, right). Backbone is depicted as a transparent cartoon and contacts on the Fabs are shown as sticks. Mutational differences between PRO-37587 and S2X259 Fabs that make contacts at the interface are highlighted. SARS-CoV-2 RBD is colored as in (B). (D) SARS-CoV-2 Omicron BA.1 RBD contacts (left) contrasted against <t>sequence</t> conservation (right). Contacts are highlighted with text and are colored as in (B). Sequence conservation is measured as the positional sequence entropy (in bits) of 18 different SARS-CoV-2 variants, including Omicron lineages (SARS-CoV-2 WIV04, D614G, Delta, Omicron BA.1, BA.2, BA.4, BA.5, JN.1, XBB.1.1, LB.1, XBB.1.5, EG.5.1, BQ.1.1, KP.2, XBB.1.16.1, BA.2.86, KP.3 and KP.3.1.1). A higher entropy (red) indicates a larger variation of mutations at that position, and a lower entropy (cyan) indicates less variation. (E) Sequence population of SARS-CoV-2 RBD contacting residues, as measured across the variants used in (D). (F) From left to right, model of SARS-CoV-2 Omicron BA.1 spike with PRO-37587 Fabs bound to all three RBDs, a close up of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 RBD with an overlay of SARS-CoV-2 pre-Omicron RBD (PDB: 7RAL), and a close-up of the altered helix conformation observed in our structure and previously reported. Molecules are colored as in (B), with SARS-CoV-2 pre-Omicron RBD reference colored orange.
Deep Sequencing Data, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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deep sequencing data - by Bioz Stars, 2026-04
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(A) Cryo-EM density map of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 spike trimer. Spike chains (S chain A, B or C) are colored either gray, cyan, or green. PRO-37587 Fab heavy (HC) and light (LC) chains are colored brown and beige, respectively. (B) Molecular model of PRO-37587 Fab bound to a single SARS-CoV-2 Omicron BA.1 RBD. PRO-37587 Fab backbone is depicted as cartoon with colors as shown in (A). SARS-CoV-2 RBD is depicted with a surface representation, with contacts colored red, non-contacts colored cyan, and glycans colored orange. Contacting residues on the RBD are highlighted. (C) Comparison of contacting residues for PRO-37587 Fab (PDB: 9NFT, left) and reference molecule S2X259 Fab (PDB: 7RAL, right). Backbone is depicted as a transparent cartoon and contacts on the Fabs are shown as sticks. Mutational differences between PRO-37587 and S2X259 Fabs that make contacts at the interface are highlighted. SARS-CoV-2 RBD is colored as in (B). (D) SARS-CoV-2 Omicron BA.1 RBD contacts (left) contrasted against sequence conservation (right). Contacts are highlighted with text and are colored as in (B). Sequence conservation is measured as the positional sequence entropy (in bits) of 18 different SARS-CoV-2 variants, including Omicron lineages (SARS-CoV-2 WIV04, D614G, Delta, Omicron BA.1, BA.2, BA.4, BA.5, JN.1, XBB.1.1, LB.1, XBB.1.5, EG.5.1, BQ.1.1, KP.2, XBB.1.16.1, BA.2.86, KP.3 and KP.3.1.1). A higher entropy (red) indicates a larger variation of mutations at that position, and a lower entropy (cyan) indicates less variation. (E) Sequence population of SARS-CoV-2 RBD contacting residues, as measured across the variants used in (D). (F) From left to right, model of SARS-CoV-2 Omicron BA.1 spike with PRO-37587 Fabs bound to all three RBDs, a close up of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 RBD with an overlay of SARS-CoV-2 pre-Omicron RBD (PDB: 7RAL), and a close-up of the altered helix conformation observed in our structure and previously reported. Molecules are colored as in (B), with SARS-CoV-2 pre-Omicron RBD reference colored orange.

Journal: bioRxiv

Article Title: Functional and structural characterization of a combination of pan-sarbecovirus antibodies with potent antiviral activity

doi: 10.1101/2025.03.30.646023

Figure Lengend Snippet: (A) Cryo-EM density map of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 spike trimer. Spike chains (S chain A, B or C) are colored either gray, cyan, or green. PRO-37587 Fab heavy (HC) and light (LC) chains are colored brown and beige, respectively. (B) Molecular model of PRO-37587 Fab bound to a single SARS-CoV-2 Omicron BA.1 RBD. PRO-37587 Fab backbone is depicted as cartoon with colors as shown in (A). SARS-CoV-2 RBD is depicted with a surface representation, with contacts colored red, non-contacts colored cyan, and glycans colored orange. Contacting residues on the RBD are highlighted. (C) Comparison of contacting residues for PRO-37587 Fab (PDB: 9NFT, left) and reference molecule S2X259 Fab (PDB: 7RAL, right). Backbone is depicted as a transparent cartoon and contacts on the Fabs are shown as sticks. Mutational differences between PRO-37587 and S2X259 Fabs that make contacts at the interface are highlighted. SARS-CoV-2 RBD is colored as in (B). (D) SARS-CoV-2 Omicron BA.1 RBD contacts (left) contrasted against sequence conservation (right). Contacts are highlighted with text and are colored as in (B). Sequence conservation is measured as the positional sequence entropy (in bits) of 18 different SARS-CoV-2 variants, including Omicron lineages (SARS-CoV-2 WIV04, D614G, Delta, Omicron BA.1, BA.2, BA.4, BA.5, JN.1, XBB.1.1, LB.1, XBB.1.5, EG.5.1, BQ.1.1, KP.2, XBB.1.16.1, BA.2.86, KP.3 and KP.3.1.1). A higher entropy (red) indicates a larger variation of mutations at that position, and a lower entropy (cyan) indicates less variation. (E) Sequence population of SARS-CoV-2 RBD contacting residues, as measured across the variants used in (D). (F) From left to right, model of SARS-CoV-2 Omicron BA.1 spike with PRO-37587 Fabs bound to all three RBDs, a close up of PRO-37587 Fab bound to SARS-CoV-2 Omicron BA.1 RBD with an overlay of SARS-CoV-2 pre-Omicron RBD (PDB: 7RAL), and a close-up of the altered helix conformation observed in our structure and previously reported. Molecules are colored as in (B), with SARS-CoV-2 pre-Omicron RBD reference colored orange.

Article Snippet: Illumina-based deep sequencing data from the resistance passaging experiments are available from the NCBI Sequence Read Archive (SRA), BioProject: PRJNA1234183, BioSample Accession Numbers: SAMN47134171-SAMN47134194.

Techniques: Cryo-EM Sample Prep, Comparison, Sequencing

Combination of GB-0669 and PRO-37587 increases barrier of resistance to SARS-CoV-2 Omicron BA.1 live virus escape. GB-0669 and PRO-37587 were tested in vitro as single agents and combination with SARS-CoV-2 Omicron BA.1 live virus for 10 passages. (A) Table indicating passage number (reported as P x , with x being the passage number) and associated mutations that resulted in the virus partially or completely escaping neutralizing activity of the mAbs. Mutation coordinates are relative to the SARS-CoV-2 Omicron BA.1 spike sequence. Complete and partial escape were determined by cytopathic effect (CPE) at each passage. CPE observed at an antibody concentration of 10 µg/ml or above was considered complete escape (indicated with red), while CPE observed at antibody concentrations between 1-10 µg/ml was considered partial escape (indicated as yellow). CPE observed at antibody concentrations < 1 µg/ml, or no CPE, was considered no escape (indicated as gray). (B) Table reporting EC50 (µg/ml) values of GB-0669 and PRO-37587 as single agent and in combination (GB-0669 + PRO-37587) against resistance passaged viruses harvested after emergence of resistance for GB-0669 and PRO-37587 as single agents (respectively at P3 [GB-0669 P3 virus] and P10 [PRO-37587 P10 virus]) or at P10 for the GB-0669 + PRO-37587 combination (GB-0669 + PRO-37587 P10 virus). Viruses passaged with no antibodies and harvested at P3 (untreated P3 virus) and P10 (untreated P10 virus) were used as controls. (C) Table reporting relative frequencies of mutations identified in the escape experiment outlined in (A) among publicly available SARS-CoV-2 genomes representative of the indicated time periods (March [Mar] 2023 - Mar 2024, or January [Jan] 2020 - Mar 2024). Mutation coordinates are referenced relative to the SARS-CoV-2 Omicron BA.1 spike protein. (D) Replication kinetics (TCID50/ml) of the PRO-37587 P10 virus and the respective control (untreated P10 virus). Results are shown as mean ± SD (representative of one experiment, two technical replicates). ** indicates p ≤ 0.01 when comparing PRO-37587 P10 virus and untreated P10 virus across the same timepoints.

Journal: bioRxiv

Article Title: Functional and structural characterization of a combination of pan-sarbecovirus antibodies with potent antiviral activity

doi: 10.1101/2025.03.30.646023

Figure Lengend Snippet: Combination of GB-0669 and PRO-37587 increases barrier of resistance to SARS-CoV-2 Omicron BA.1 live virus escape. GB-0669 and PRO-37587 were tested in vitro as single agents and combination with SARS-CoV-2 Omicron BA.1 live virus for 10 passages. (A) Table indicating passage number (reported as P x , with x being the passage number) and associated mutations that resulted in the virus partially or completely escaping neutralizing activity of the mAbs. Mutation coordinates are relative to the SARS-CoV-2 Omicron BA.1 spike sequence. Complete and partial escape were determined by cytopathic effect (CPE) at each passage. CPE observed at an antibody concentration of 10 µg/ml or above was considered complete escape (indicated with red), while CPE observed at antibody concentrations between 1-10 µg/ml was considered partial escape (indicated as yellow). CPE observed at antibody concentrations < 1 µg/ml, or no CPE, was considered no escape (indicated as gray). (B) Table reporting EC50 (µg/ml) values of GB-0669 and PRO-37587 as single agent and in combination (GB-0669 + PRO-37587) against resistance passaged viruses harvested after emergence of resistance for GB-0669 and PRO-37587 as single agents (respectively at P3 [GB-0669 P3 virus] and P10 [PRO-37587 P10 virus]) or at P10 for the GB-0669 + PRO-37587 combination (GB-0669 + PRO-37587 P10 virus). Viruses passaged with no antibodies and harvested at P3 (untreated P3 virus) and P10 (untreated P10 virus) were used as controls. (C) Table reporting relative frequencies of mutations identified in the escape experiment outlined in (A) among publicly available SARS-CoV-2 genomes representative of the indicated time periods (March [Mar] 2023 - Mar 2024, or January [Jan] 2020 - Mar 2024). Mutation coordinates are referenced relative to the SARS-CoV-2 Omicron BA.1 spike protein. (D) Replication kinetics (TCID50/ml) of the PRO-37587 P10 virus and the respective control (untreated P10 virus). Results are shown as mean ± SD (representative of one experiment, two technical replicates). ** indicates p ≤ 0.01 when comparing PRO-37587 P10 virus and untreated P10 virus across the same timepoints.

Article Snippet: Illumina-based deep sequencing data from the resistance passaging experiments are available from the NCBI Sequence Read Archive (SRA), BioProject: PRJNA1234183, BioSample Accession Numbers: SAMN47134171-SAMN47134194.

Techniques: Virus, In Vitro, Activity Assay, Mutagenesis, Sequencing, Concentration Assay, Control