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Journal: The Journal of Biological Chemistry
Article Title: CXCR4 Receptor Overexpression in Mesenchymal Stem Cells Facilitates Treatment of Acute Lung Injury in Rats
doi: 10.1074/jbc.M114.605063
Figure Lengend Snippet: Localization and migration of transplanted CXCR4-MSCs in the lungs. Lung tissue of ALI rats was harvested for immunocytochemistry with anti-CXCR4 and anti-Ki67 antibodies 2 weeks post MSC injection. A, GFP+ MSCs (green) were observed in the lung tissue in both cases, and the majority of GFP+ MSCs were Ki67-positive (red), corresponding to the proliferation potential of the cells. B, CXCR4 was strongly expressed (red) only in the lung of rats treated with CXCR4-MSCs. The white arrows highlight cells that are double-positive to CXCR4 and GFP. Nuclei were stained with DAPI (blue). Scale bar: 100 in A, 50 μm in B. C, quantitative analysis of percentages of Ki67+/GFP+/DAPI+ cells in the total number of GFP+/DAPI+ cells in lungs of ALI rats. D, quantitative analysis of the number of GFP+ MSCs (cells/mm2) in lungs of ALI rats. Symbols represent the mean ± S.D.; n = 5 rats per group, 5 sections per rat were selected, and 10 fields were photographed in each section. Cells were counted by Image J software. Comparison between groups of CXCR4-MSCs and GFP-MSCs. **, p < 0.01.
Article Snippet: Rat MSCs were cultured in
Techniques: Migration, Immunocytochemistry, Injection, Staining, Software