Journal: Poultry Science

Article Title: Multi-omics analysis reveals RBPJ-mediated regulation of EGF/ACTN2 / MYPN / COL21A1 in fibroblast during oviduct functional remodeling of duck

doi: 10.1016/j.psj.2026.106688

Figure Lengend Snippet: Identification of duck embryo fibroblasts and the effects of RBPJ overexpression on proliferation. (A) Immunofluorescence identification of decorin and vimentin in duck embryo fibroblasts. Target proteins (red, Alexa Fluor 488), nuclei (blue, Hochest 33342). Scale bar =100 μm. (B) Relative expression level of RBPJ mRNA, plot with Control as 1. (C) GAPDH and RBPJ protein band diagrams. (D) Quantified relative protein bands, plot with Control as 1. (E) Cell viability curve after RBPJ overexpression. (F) Staining maps of Group C and OE. (G) Quantitative plot of the proportion of EdU positive cells in two groups. pCD3.1 is the plasmid of group C and pCD3.1-RBPJ is the plasmid of group OE of RBPJ ( ⁎⁎ P < 0.01; ⁎⁎⁎ P < 0.001; ⁎⁎⁎⁎ P < 0.0001).

Article Snippet: To minimized non-specific binding, cells were blocked with BeyoFCTM FcR Blocking Solution (C1752S, Beyotime, China) for 15 minutes at RT. was After blocking, cells were incubated overnight at 4°Cwith the following primary antibodies diluted in Dilution Buffer: Vimentin (mouse monoclonal antibody, 1:100, 60330-1-Ig, Proteintech, China) or Decorin (mouse monoclonal antibody, 1:100, 66847-1-Ig, Proteintech, China).

Techniques: Over Expression, Immunofluorescence, Expressing, Control, Staining, Plasmid Preparation