Journal: Materials Today Bio

Article Title: Neuro-bone-skin tri-regeneration via a microenvironment-responsive PRP-loaded chitosan hydrogel for traumatic brain injury therapy

doi: 10.1016/j.mtbio.2026.102913

Figure Lengend Snippet: Pro-angiogenic and neuroregenerative effects with anti-apoptotic activity of CO@P hydrogel. A1-E1) Immunofluorescence of VEGF, CD31, DCX, Neun and Caspase3 in different groups. For panoramic figures, scale bar = 1000 μm; for enlarged figures, scale bar = 200 μm. A2-E2) Fluorescence intensity of VEGF, CD31, DCX, Neun and Caspase3 in each group. Data were presented as mean ± SEM N = 3 per group. ∗: P < 0.05 compared with Sham group; #: P < 0.05 compared with TBI group; Δ: P < 0.05 compared with CO@P group.

Article Snippet: Primary antibodies used included NeuN (1100 μg/mL), GFAP (800 μg/mL), Iba1 (700 μg/mL), iNOS (820 μg/mL), Arg1 (900 μg/mL), VEGF (1000 μg/mL), CD31 (600 μg/mL), Caspase-3 (700 μg/mL), DCX (600 μg/mL) (1:200 dilution; Proteintech, China).

Techniques: Activity Assay, Immunofluorescence, Fluorescence