Journal: Environmental toxicology

Article Title: Picrasidine I Regulates Apoptosis in Melanoma Cell Lines by Activating ERK and JNK Pathways and Suppressing AKT Signaling.

doi: 10.1002/tox.24404

Figure Lengend Snippet: FIGURE 4 | Influence of picrasidine I on intrinsic apoptotic pathway and mitochondrial membrane depolarization in melanoma cells. (A) Flow cytometric analysis of depolarized cells in HMY-1 and A2058 cell lines. (B) Quantitation of total depolarized cells. (C) Western blot analysis of TRADD, FADD, Fas, DR5, and DcR3, with β-actin as the internal control. (D) Western blot analysis of Bax, Bak, Bcl-2, Bcl-xL, t-bid, and Bim-L/S, with β-actin as the internal control. (E, F) Expression levels of apoptotic proteins associated with the extrinsic pathway in picrasidine I-treated HMY- 1 and A2058 cell lines. (G, H) Expression levels of apoptotic proteins associated with the intrinsic pathway in picrasidine I-treated HMY-1 and A2058. All data are presented as mean ± standard deviation (n = 3). *p < 0.05, indicating statistical significance compared with the control group.

Article Snippet: The primary antibodies against cyclin A2 #4656, cyclin D1 #2978, CDK2 #2546, CDK4 #12790, CDK6 #3136, TRADD #3684, FADD #2782, Fas #4233, DR5 #8074, DcR3 #4758, Bax #5023, Bcl- 2 #4223, Bcl- xL #2764, Bid #2002, Bim #2933, ccaspase 3 #9664, c- caspase 8 #9496, c- caspase 9 #9502, p- AKT #4060, AKT #2920, p- ERK #4370, ERK #4695, p38 #9212, pJNK #4668, JNK #9258, claspin #2800, and β- actin #4970 were purchased from Cell Signaling Technology, Danvers, MA, USA; antibody against p- p38 #44- 684G was purchased from 15227278, 0, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/tox.24404 by IN A SP - N E PA L , W iley O nline L ibrary on [04/09/2024].

Techniques: Membrane, Quantitation Assay, Western Blot, Control, Expressing, Standard Deviation