Journal: Renal Failure

Article Title: Efficacy of nicotiflorin in ameliorating septic acute kidney injury: the role of PINK1/parkin in mitochondrial restoration and oxidative stress reduction

doi: 10.1080/0886022X.2026.2624206

Figure Lengend Snippet: The effect of nicotiflorin on viability, apoptosis and oxidative stress in LPS-treated NRK-52E cells. NRK-52E were treated with/without LPS (10 µg/ml) and/or nicotiflorin (75 µg/ml) for 24 h. (A) CCK-8 assay was employed to assess cell viability. (B–C) Annexin V-FITC/propidium iodide staining combined with flow cytometry was used to analyze cell apoptosis. (D–E) DCFH-DA probe combined with flow cytometry was applied to measure ROS content. (F-G) MitoSOX Red superoxide indicator combined with flow cytometry was used to detect the production of mitochondrial superoxide. *** p < 0.001 vs. Control; ^ p < 0.05, ^^ p < 0.01, ^^^ p < 0.001 vs. Nicotiflorin; # p < 0.05, ## p < 0.01 vs. LPS. n = 3, measurement data expressed as mean ± standard deviation. Difference comparisons among multiple groups were analyzed using one-way analysis of variance. ROS: reactive oxygen species; CCK-8: cell counting kit 8; LPS: lipopolysaccharide.

Article Snippet: For measuring ROS content, the cells were suspended with PBS and incubated with DCFH-DA probe (HY-D0940, MedChemExpress, Monmouth Junction, NJ, USA) at 37 °C for 1 h away from light.

Techniques: CCK-8 Assay, Staining, Flow Cytometry, Control, Standard Deviation, Cell Counting