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data cut run data  (Jackson Laboratory)

 
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    Jackson Laboratory data cut run data
    Data Cut Run Data, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/data+cut+run+data/cut+data+data+run/pm41916278-261-177-192
    Average 86 stars, based on 1 article reviews
    data cut run data - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Staining:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    Membrane:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    Gel Extraction:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    DNA Purification:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    Chromatin Immunoprecipitation:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    DNA Sequencing:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    Multiplex Assay:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    Recombinant:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2

    Plasmid Preparation:

    Article Title: Enhancers that direct gene expression to central nervous system vascular endothelial cells in vivo.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER LunaScript RT Master Mix Kit New England BioLabs Cat. # E3025L RNase H New England BioLabs Cat. # M0297S Random 6-mer primers New England BioLabs Cat. # S1230S Novex pre-poured TBE Gels (10% acrylamide, 10 wells) Thermo Fisher Cat. # EC6275BOX GelStar Nucleic Acid Gel Stain Lonza Cat. # 50535 Spin-X Centrifuge Tube Filter CA membrane Corning Costar Cat. # 07-200-388 QIAquick Gel Extraction Kit Qiagen Cat. # 28706 Q5 High-Fidelity DNA Polymerase and Q5 High GC Enhancer New England BioLabs Cat. # M0491S CUT&RUN Assay Kit Cell Signaling Technology Cat. # 86652 DNA Purification Buffers and Spin Columns Cell Signaling Technology Cat. # 14209 SimpleChIP ChIP-seq DNA Library Prep Kit for the Illumina platform Cell Signaling Technology Cat. # 56795 AMPure XP Beads and Bead Reagents Beckman Coulter Life Sciences Cat. # A63880 Critical commercial assays DNA sequencing on a NextSeq 500 and on a NovaSeq X Plus Illumina N/A Oxford Nanopore DNA sequencing service Plasmidsaurus https://www.plasmidsaurus. com/home rAAV production Viral Tools core laboratory, Janelia Research Campus (HHMI) https://www.janelia.org/ support-team/viral-tools Deposited data CUT&RUN data (https://www.ncbi.nlm.nih. gov/sra) This paper ID PRJNA1277276 Experimental models: Organisms/strains C57BL6/J mice (pigmented) Jackson Laboratories JAX 000664 C57BL6/J mice (albino) Jackson Laboratories JAX 000058 B6J.129 R26-Cas9-GFP mice Jackson Laboratories JAX 026179 Tie2-GFP mice Jackson Laboratories JAX 008863 Oligonucleotides sgRNAs, DNA oligonucleotides, gene blocks Integrated DNA Technologies (IDT) N/A Synthetic 300-mer library Twist BioScience N/A SimpleChIP ChIP-seq Multiplex Oligonucleotides for the Illumina platform (Single Index Primers) Cell Signaling Technology Cat. # 29580 Recombinant DNA lacZ reporter plasmid Addgene, Kvon et al. 34 Cat. # 139098 pAAV-CAG-SV40NLSf-GFP-3xmiR122WPRE-HGHpA plasmid Addgene Cat. # 183775 NanoLuc Reporter Vector plasmid Promega Cat. # N1111 kiCAP-AAV-BI30 plasmid Addgene Cat # 183749 pUCmini-iCAP-PHP.B plasmid Addgene Cat. # 103002 pmU6-gRNA plasmid Addgene Cat. # 53187 pLentiCRISPRv2-blast plasmid Addgene Cat. # 98293 phH1-gRNA plasmid Addgene Cat. # 53186 ph7SK-gRNA plasmid Addgene Cat. # 53189 (Continued on next page) Neuron 114, 1–15.e1–e9, May 20, 2026 e2



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    EpiCypher data analysis cut run 77
    Chrom-Sig results on <t>GM12878</t> CTCF ChIP-seq and CUT&RUN datasets. (a) Browser views of CTCF binding motifs with orientation (blue triangles) and coverage tracks generated by piling up the original (before Chrom-Sig) and Chrom-Sig ‘pass’ or ‘fail’ reads, accompanied by the peaks called by SICER. (b) Venn diagram of the peaks called on the original and Chrom-Sig ‘pass’ reads pile-up reads using false discovery rate (FDR) of 0.1 and 5000 pseudo-reads, with boxplots of maximum peak intensity for each peak. (c) Number of peaks overlapping CTCF motifs, and top MEME result on the original GM12878 CTCF CUT&RUN data before Chrom-Sig. (d) Similar to panel c for Chrom-Sig ‘pass’ results.
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    Chrom-Sig results on GM12878 CTCF ChIP-seq and CUT&RUN datasets. (a) Browser views of CTCF binding motifs with orientation (blue triangles) and coverage tracks generated by piling up the original (before Chrom-Sig) and Chrom-Sig ‘pass’ or ‘fail’ reads, accompanied by the peaks called by SICER. (b) Venn diagram of the peaks called on the original and Chrom-Sig ‘pass’ reads pile-up reads using false discovery rate (FDR) of 0.1 and 5000 pseudo-reads, with boxplots of maximum peak intensity for each peak. (c) Number of peaks overlapping CTCF motifs, and top MEME result on the original GM12878 CTCF CUT&RUN data before Chrom-Sig. (d) Similar to panel c for Chrom-Sig ‘pass’ results.

    Journal: Bioinformatics

    Article Title: Chrom-Sig: de-noising 1D genomic profiles by signal processing methods

    doi: 10.1093/bioinformatics/btaf645

    Figure Lengend Snippet: Chrom-Sig results on GM12878 CTCF ChIP-seq and CUT&RUN datasets. (a) Browser views of CTCF binding motifs with orientation (blue triangles) and coverage tracks generated by piling up the original (before Chrom-Sig) and Chrom-Sig ‘pass’ or ‘fail’ reads, accompanied by the peaks called by SICER. (b) Venn diagram of the peaks called on the original and Chrom-Sig ‘pass’ reads pile-up reads using false discovery rate (FDR) of 0.1 and 5000 pseudo-reads, with boxplots of maximum peak intensity for each peak. (c) Number of peaks overlapping CTCF motifs, and top MEME result on the original GM12878 CTCF CUT&RUN data before Chrom-Sig. (d) Similar to panel c for Chrom-Sig ‘pass’ results.

    Article Snippet: As visually speculated for GM12878 CTCF CUT&RUN data (4DNFI2G71DR4), there were originally 55 251 peaks, of which 16 757 (42.7%) were recovered by Chrom-Sig ; it also identified a small number (17) of new peaks ( ).

    Techniques: ChIP-sequencing, Binding Assay, Generated