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4 6 diamidino 2 phenylindole  (Dojindo Labs)


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    Structured Review

    Dojindo Labs 4 6 diamidino 2 phenylindole
    4 6 Diamidino 2 Phenylindole, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution/Cellstain+DAPI+solution/pm42863997-63-16-24
    Average 96 stars, based on 495 article reviews
    4 6 diamidino 2 phenylindole - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: MicroRNA-33 inhibition ameliorates muscular dystrophy by enhancing skeletal muscle regeneration
    Article Snippet: DAPI solution , DOJINDO , D523.

    Article Title: Trajectory analysis reveals an uncommitted neuroblastic state in MYCN-driven neuroblastoma development.
    Article Snippet: They were then incubated with rabbit anti-MYCN (D1V2A) antibody (1:100, 84406, Cell Signaling Technology) in antibody diluent (1% BSA in 0.3% Triton X-100/D-PBS) overnight at 4 °C.


    Article Title: Spatial proteomics reveals prefrontal circuit diversity in socioemotional behaviour
    Article Snippet: The following antibodies were used: rabbit anti-HA (Cell Signaling Technology, C29F4), rabbit anti-Homer1 (Synaptic Systems, 160-003), guinea pig anti-VGLUT-1 (Synaptic Systems, 135-304), monoclonal α-Tubulin (Santa Cruz, sc-32293), rabbit anti-KIAA0100 (BLTP2) (MyBioSource, MBS8243055), rabbit anti-mCherry (Abcam, ab167453), rabbit anti-Neurexin-1α (Nittobo Medical, MSFR104630), chicken anti-GFP (Merck, AB16901), Alexa Fluor 488 Goat anti-Mouse (ThermoFisher, A32723), Alexa Fluor 488 Goat anti-Rabbit (ThermoFisher, A-11008), Alexa Fluor 488 Goat anti-Guinea pig (ThermoFisher, A11073), Alexa Fluor 488 Goat anti-Chicken (ThermoFisher, A-11039), Alexa Fluor 555 Goat anti-Rabbit (ThermoFisher, A21428), Alexa Fluor 555 Goat anti-Guinea pig (ThermoFisher, A21435), Alexa Fluor 647 Donkey anti-rabbit (ThermoFisher, A31573), Alexa Fluor 647 Goat anti-Chicken (ThermoFisher, A-21449), Alexa Fluor 647 Donkey anti-Guinea pig (Jackson ImmunoResearch, 706-605-148), Alexa Fluor 647 Streptavidin (ThermoFisher, S21374), StarBright Blue 700 anti-Mouse (BioRad, 12004159), StarBright Blue 700 anti-Rabbit (BioRad, 12004162), CF770 anti-Mouse (Biotium, 20077), CF770 anti-Rabbit (Biotium, 20078) and DAPI solution (Dojindo, 340-07971).

    Staining:

    Article Title: Thiol methyltransferase METTL7B coordinates lipid metabolism and promotes tumor progression in pancreatic cancer
    Article Snippet: Samples were then incubated at 4°C overnight with primary antibodies against METTL7B (Atlas Antibodies, HPA038644, 1:200 dilution), PDIA3 (Sigma-Aldrich, AMAB90988, 1:200 dilution), or CD29 (BD, 610467, 1:200 dilution) diluted in BSA-T. After washing three times with PBS-T, samples were incubated at room temperature for 1 hour with Alexa Fluor 488-labeled anti-rabbit IgG secondary antibody (Thermo Fisher Scientific, A21206, 1:1000 dilution), Alexa Fluor 555-labeled anti-mouse IgG secondary antibody (Thermo Fisher Scientific, A31570, 1:1000 dilution), or Alexa Fluor 647-labeled anti-mouse IgG secondary antibody (Thermo Fisher Scientific, A31571, 1:1000 dilution) diluted in BSA-T, followed by three washes with PBS-T. For LD staining, samples were incubated with 0.1 μM Lipi-Blue (Dojindo, LD01) or Lipi-Deep Red (Dojindo, LD04) in PBS at 37°C for 1 hour. .. For nuclear staining, samples were incubated with 1 μg/mL DAPI solution (Dojindo, D523) in PBS for 10 minutes at room temperature. .. After washing three times with PBS, samples were mounted with ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific, P36934) for imaging.

    Article Title: Nuclear enlargement after confined migration in cancer cells
    Article Snippet: .. The cells were then washed again with PBS and stained with DAPI solution (Dojindo, D523) in water (1/5000) for 30 minutes at room temperature. .. After additional washing with Milli-Q water, 20 μL of DAPI Fluoromount-GR (Southern Biotech) was applied to the cells.

    Incubation:

    Article Title: Thiol methyltransferase METTL7B coordinates lipid metabolism and promotes tumor progression in pancreatic cancer
    Article Snippet: Samples were then incubated at 4°C overnight with primary antibodies against METTL7B (Atlas Antibodies, HPA038644, 1:200 dilution), PDIA3 (Sigma-Aldrich, AMAB90988, 1:200 dilution), or CD29 (BD, 610467, 1:200 dilution) diluted in BSA-T. After washing three times with PBS-T, samples were incubated at room temperature for 1 hour with Alexa Fluor 488-labeled anti-rabbit IgG secondary antibody (Thermo Fisher Scientific, A21206, 1:1000 dilution), Alexa Fluor 555-labeled anti-mouse IgG secondary antibody (Thermo Fisher Scientific, A31570, 1:1000 dilution), or Alexa Fluor 647-labeled anti-mouse IgG secondary antibody (Thermo Fisher Scientific, A31571, 1:1000 dilution) diluted in BSA-T, followed by three washes with PBS-T. For LD staining, samples were incubated with 0.1 μM Lipi-Blue (Dojindo, LD01) or Lipi-Deep Red (Dojindo, LD04) in PBS at 37°C for 1 hour. .. For nuclear staining, samples were incubated with 1 μg/mL DAPI solution (Dojindo, D523) in PBS for 10 minutes at room temperature. .. After washing three times with PBS, samples were mounted with ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific, P36934) for imaging.

    Article Title: Trajectory analysis reveals an uncommitted neuroblastic state in MYCN-driven neuroblastoma development
    Article Snippet: They were then incubated with rabbit anti-MYCN (D1V2A) antibody (1:100, 84406, Cell Signaling Technology) in antibody diluent (1% BSA in 0.3% Triton X-100/D-PBS) overnight at 4 °C. .. This was followed by incubation with an Alexa Fluor 594-labeled goat anti-rabbit IgG secondary antibody (1:500, A-11012, Thermo Fisher Scientific) and DAPI solution (1:1000, D523, Dojindo) for 2 h at RT. .. Slides were mounted with Prolong Gold Antifade Mountant ( P10144 , Thermo Fisher Scientific) and imaged using an inverted microscope (BX41, EVIDENT) with the VS129 virtual slide system (EVIDENT).



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    Image Search Results


    Journal: iScience

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa

    doi: 10.1016/j.isci.2026.117730

    Figure Lengend Snippet:

    Article Snippet: Finally, sections were counterstained with Diamidin-2-phenylindol (DAPI, ACD Bio-Techne GmbH) for 30 seconds at RT and mounted directly using ProLong Gold antifade mounting medium (Thermo Fisher Scientific, USA).

    Techniques: Plasmid Preparation, Virus, Indirect ELISA, Recombinant, RNAscope, Multiplex Assay, Software, Negative Control, Confocal, Laser-Scanning Microscopy

    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing