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counterstaining with dapi  (Beyotime)


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    Structured Review

    Beyotime counterstaining with dapi
    Counterstaining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+counterstaining/DAPI/10__1007_slash_s13402___026___01300___8-124-14-18
    Average 99 stars, based on 32901 article reviews
    counterstaining with dapi - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis
    Article Snippet: .. After blocking, sections were incubated with transferase dUTP-mediated nick-end labeling (TUNEL) reaction mixture (50 μL/section) overnight at 37 °C, followed by DAPI counterstaining (C1005, Beyotime). ..

    Incubation:

    Article Title: Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis
    Article Snippet: .. After blocking, sections were incubated with transferase dUTP-mediated nick-end labeling (TUNEL) reaction mixture (50 μL/section) overnight at 37 °C, followed by DAPI counterstaining (C1005, Beyotime). ..

    Article Title: Macrophage-mimetic liposomes co-delivering ceria and Ac2-26 peptide for penumbra protection in ischemic stroke.
    Article Snippet: Samples were incubated overnight at 4 °C with primary antibodies against ZO-1 (Invitrogen, 61-7300, 1:50), NeuN (Abcam, ab104224, 1:400), or Nrf2 (Proteintech, 16396-1-AP, 1:500). .. After washing, samples were incubated with fluorophore-conjugated secondary antibodies (Invitrogen, 1:500) for 2 h at room temperature, followed by DAPI counterstaining (Beyotime, China). .. Imaging was performed using an Olympus FV3000 confocal laser scanning microscope.

    Labeling:

    Article Title: Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis
    Article Snippet: .. After blocking, sections were incubated with transferase dUTP-mediated nick-end labeling (TUNEL) reaction mixture (50 μL/section) overnight at 37 °C, followed by DAPI counterstaining (C1005, Beyotime). ..

    TUNEL Assay:

    Article Title: Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis
    Article Snippet: .. After blocking, sections were incubated with transferase dUTP-mediated nick-end labeling (TUNEL) reaction mixture (50 μL/section) overnight at 37 °C, followed by DAPI counterstaining (C1005, Beyotime). ..

    Article Title: Targeted neural stem cell-derived extracellular vesicles loaded with Sinomenine alleviate diabetic peripheral neuropathy via WNT5a/TRPV1 pathway modulation
    Article Snippet: Cells were fixed with 4% paraformaldehyde (Yeasen, #60536ES60) for 15 min, permeabilized with 0.25% Triton X-100 for 20 min, and blocked with 5% BSA (Yeasen, #36101ES25). .. Staining was carried out using a TUNEL kit (Beyotime, #C1086), followed by DAPI counterstaining (Beyotime, #C1002). ..

    Article Title: TNIP3 Attenuates Colitis by Inhibiting the TAB1/NF-κB/NLRP3 Signaling Pathway in Macrophages.
    Article Snippet: Background Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, is characterized by chronic, recurrent, and non-specific intestinal inflammation.. This study aimed to elucidate the mechanism of TNIP3 (TNFAIP3interacting protein 3) in modulating macrophage (Mφ) polarization and intestinal epithelial barrier function in IBD.. Methods LPS-induced RAW 264.7 cells and DSS-induced mouse models were used to investigate the role of TNIP3 in IBD.

    Staining:

    Article Title: Targeted neural stem cell-derived extracellular vesicles loaded with Sinomenine alleviate diabetic peripheral neuropathy via WNT5a/TRPV1 pathway modulation
    Article Snippet: Cells were fixed with 4% paraformaldehyde (Yeasen, #60536ES60) for 15 min, permeabilized with 0.25% Triton X-100 for 20 min, and blocked with 5% BSA (Yeasen, #36101ES25). .. Staining was carried out using a TUNEL kit (Beyotime, #C1086), followed by DAPI counterstaining (Beyotime, #C1002). ..

    Fluorescence:

    Article Title: Ginkgetin Alleviates Doxorubicin-Induced Heart Failure by Regulating Mitochondrial Dysfunction Through the AMPK/Sirt1/NF-κB Signaling Pathway
    Article Snippet: Samples were permeabilized with 0.1% Triton X-100 for 10 minutes prior to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining ( C10617 , Invitrogen) at 37 °C for 1 hour. .. After DAPI counterstaining for 5 minutes and mounting with P0126 (Beyotime), apoptotic cells were quantified in five randomly selected fields under a fluorescence microscope. ..

    Microscopy:

    Article Title: Ginkgetin Alleviates Doxorubicin-Induced Heart Failure by Regulating Mitochondrial Dysfunction Through the AMPK/Sirt1/NF-κB Signaling Pathway
    Article Snippet: Samples were permeabilized with 0.1% Triton X-100 for 10 minutes prior to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining ( C10617 , Invitrogen) at 37 °C for 1 hour. .. After DAPI counterstaining for 5 minutes and mounting with P0126 (Beyotime), apoptotic cells were quantified in five randomly selected fields under a fluorescence microscope. ..



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    (a) Actin (green) and <t>DAPI</t> (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.
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    Image Search Results


    Age-dependent impairment in myelin phagocytosis and lipid droplet clearance in vitro and in vivo (Ai-ii) In vitro myelin phagocytosis assay with primary peritoneal macrophages from young (1-month-old) and aged (12-month-old) mice. (Ai) Representative immunofluorescence cytochemistry images (myelin basic protein (MBP, green), Iba1 (red), and DAPI (blue) of macrophages from young and aged mice incubated with myelin debris for 2, 4, 16, or 24 h. Scale bar: 20μm. (Aii) Quantification of macrophages containing phagocytosed myelin (% of MBP-positive Iba1- macrophages) at early (2–4 h) and late (16–24 h) time points. (Bi-iii) Delayed metabolism of phagocytosed myelin by aged macrophages. (Bi) Experimental scheme showing removal of myelin from culture medium after 16 h, and assessment of intracellular myelin metabolism by accumulation of Oil-Red-O (ORO) lipid droplets at 24 and 48 h. As an experimental control, representative images of MBP (green), Iba1 (red), and DAPI (blue) (Scale bar: 20μm) in young and aged macrophages showing comparable levels of phagocytosed MBP-immunoreactive myelin in young and aged macrophages at 16 h, and absence of MBP-immunoreactive myelin at 24 h and 48 h, (Bii) ORO and hematoxylin staining of young and aged macrophages showing ORO+ lipid droplet accumulation (red, arrowheads). (Biii) Quantification of ORO+ lipid–containing macrophages following myelin incubation and withdrawal, demonstrating impaired lipid clearance in aged compared to young macrophages. Scale bar: 3μm. (Ci-iii) In vivo accumulation of ORO+ lipid droplets in young (2-month-old) and old (24-month-old) mice during cuprizone-induced demyelination. (Ci) Representative ORO-stained coronal brain sections from young and aged mice after 4 (CPZ4), 6 (CPZ6) and 8 (CPZ8) weeks of continuous cuprizone feeding, and CPZ6 followed by 4 weeks of recovery after cuprizone withdrawal (CPZ6+4). Scale bar: 10μm. Quantification of ORO+ lipid droplets in, (Cii) the corpus callosum (CC) and (Ciii) dorsal fornix (DF) between young and aged mice at CPZ0, CPZ4, CPZ6, CPZ6+4 and CPZ8. (n = 5 mice/group; Mean ± SEM, p<0.05, *p<0.01, **p<0.001, ***p<0.0001).

    Journal: bioRxiv

    Article Title: Age-related loss of brain demyelinating and remyelinating potential is overcome by microglia renewal

    doi: 10.64898/2026.06.15.732390

    Figure Lengend Snippet: Age-dependent impairment in myelin phagocytosis and lipid droplet clearance in vitro and in vivo (Ai-ii) In vitro myelin phagocytosis assay with primary peritoneal macrophages from young (1-month-old) and aged (12-month-old) mice. (Ai) Representative immunofluorescence cytochemistry images (myelin basic protein (MBP, green), Iba1 (red), and DAPI (blue) of macrophages from young and aged mice incubated with myelin debris for 2, 4, 16, or 24 h. Scale bar: 20μm. (Aii) Quantification of macrophages containing phagocytosed myelin (% of MBP-positive Iba1- macrophages) at early (2–4 h) and late (16–24 h) time points. (Bi-iii) Delayed metabolism of phagocytosed myelin by aged macrophages. (Bi) Experimental scheme showing removal of myelin from culture medium after 16 h, and assessment of intracellular myelin metabolism by accumulation of Oil-Red-O (ORO) lipid droplets at 24 and 48 h. As an experimental control, representative images of MBP (green), Iba1 (red), and DAPI (blue) (Scale bar: 20μm) in young and aged macrophages showing comparable levels of phagocytosed MBP-immunoreactive myelin in young and aged macrophages at 16 h, and absence of MBP-immunoreactive myelin at 24 h and 48 h, (Bii) ORO and hematoxylin staining of young and aged macrophages showing ORO+ lipid droplet accumulation (red, arrowheads). (Biii) Quantification of ORO+ lipid–containing macrophages following myelin incubation and withdrawal, demonstrating impaired lipid clearance in aged compared to young macrophages. Scale bar: 3μm. (Ci-iii) In vivo accumulation of ORO+ lipid droplets in young (2-month-old) and old (24-month-old) mice during cuprizone-induced demyelination. (Ci) Representative ORO-stained coronal brain sections from young and aged mice after 4 (CPZ4), 6 (CPZ6) and 8 (CPZ8) weeks of continuous cuprizone feeding, and CPZ6 followed by 4 weeks of recovery after cuprizone withdrawal (CPZ6+4). Scale bar: 10μm. Quantification of ORO+ lipid droplets in, (Cii) the corpus callosum (CC) and (Ciii) dorsal fornix (DF) between young and aged mice at CPZ0, CPZ4, CPZ6, CPZ6+4 and CPZ8. (n = 5 mice/group; Mean ± SEM, p<0.05, *p<0.01, **p<0.001, ***p<0.0001).

    Article Snippet: This was followed by a three-probe amplification steps (AMP1-AMP3), fluorophore conjugation (Opal 570) and DAPI counterstaining (ACD, CA).

    Techniques: In Vitro, In Vivo, Phagocytosis Assay, Immunofluorescence, Incubation, Control, Staining

    (a) Actin (green) and DAPI (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.

    Journal: Bioengineering & Translational Medicine

    Article Title: Extracellular matrix microarchitecture modulates cellular behavior and extracellular vesicle phenotypes in biomimetic tendon models

    doi: 10.1002/btm2.70134

    Figure Lengend Snippet: (a) Actin (green) and DAPI (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.

    Article Snippet: Cellular morphology was assessed via actin staining (Invitrogen, Cat. No. A12379) and nuclear counterstaining with DAPI (Fisher Scientific, Cat. No. H‐1200‐10) ( n = 3).

    Techniques: Staining, Derivative Assay, Control