counterstaining with dapi (Beyotime)
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Counterstaining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+counterstaining/DAPI/pm41981465-107-32-35
Average 99 stars, based on 32308 article reviews
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Blocking Assay:Article Title: Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis Article Snippet: .. After blocking, sections were incubated with transferase dUTP-mediated nick-end labeling (TUNEL) reaction mixture (50 μL/section) overnight at 37 °C, followed by Incubation:Article Title: Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis Article Snippet: .. After blocking, sections were incubated with transferase dUTP-mediated nick-end labeling (TUNEL) reaction mixture (50 μL/section) overnight at 37 °C, followed by Article Title: Macrophage-mimetic liposomes co-delivering ceria and Ac2-26 peptide for penumbra protection in ischemic stroke Article Snippet: Samples were incubated overnight at 4 °C with primary antibodies against ZO-1 (Invitrogen, 61-7300, 1:50), NeuN (Abcam, ab104224, 1:400), or Nrf2 (Proteintech, 16396-1-AP, 1:500). .. After washing, samples were incubated with fluorophore-conjugated secondary antibodies (Invitrogen, 1:500) for 2 h at room temperature, followed by Article Title: Macrophage-mimetic liposomes co-delivering ceria and Ac2-26 peptide for penumbra protection in ischemic stroke. Article Snippet: Samples were incubated overnight at 4 °C with primary antibodies against ZO-1 (Invitrogen, 61-7300, 1:50), NeuN (Abcam, ab104224, 1:400), or Nrf2 (Proteintech, 16396-1-AP, 1:500). .. After washing, samples were incubated with fluorophore-conjugated secondary antibodies (Invitrogen, 1:500) for 2 h at room temperature, followed by Labeling:Article Title: Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis Article Snippet: .. After blocking, sections were incubated with transferase dUTP-mediated nick-end labeling (TUNEL) reaction mixture (50 μL/section) overnight at 37 °C, followed by TUNEL Assay:Article Title: Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis Article Snippet: .. After blocking, sections were incubated with transferase dUTP-mediated nick-end labeling (TUNEL) reaction mixture (50 μL/section) overnight at 37 °C, followed by Article Title: Targeted neural stem cell-derived extracellular vesicles loaded with Sinomenine alleviate diabetic peripheral neuropathy via WNT5a/TRPV1 pathway modulation Article Snippet: Cells were fixed with 4% paraformaldehyde (Yeasen, #60536ES60) for 15 min, permeabilized with 0.25% Triton X-100 for 20 min, and blocked with 5% BSA (Yeasen, #36101ES25). .. Staining was carried out using a TUNEL kit (Beyotime, #C1086), followed by Article Title: TNIP3 Attenuates Colitis by Inhibiting the TAB1/NF-κB/NLRP3 Signaling Pathway in Macrophages. Article Snippet: Background Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, is characterized by chronic, recurrent, and non-specific intestinal inflammation.. This study aimed to elucidate the mechanism of TNIP3 (TNFAIP3interacting protein 3) in modulating macrophage (Mφ) polarization and intestinal epithelial barrier function in IBD.. Methods LPS-induced RAW 264.7 cells and DSS-induced mouse models were used to investigate the role of TNIP3 in IBD. Staining:Article Title: Targeted neural stem cell-derived extracellular vesicles loaded with Sinomenine alleviate diabetic peripheral neuropathy via WNT5a/TRPV1 pathway modulation Article Snippet: Cells were fixed with 4% paraformaldehyde (Yeasen, #60536ES60) for 15 min, permeabilized with 0.25% Triton X-100 for 20 min, and blocked with 5% BSA (Yeasen, #36101ES25). .. Staining was carried out using a TUNEL kit (Beyotime, #C1086), followed by Fluorescence:Article Title: Ginkgetin Alleviates Doxorubicin-Induced Heart Failure by Regulating Mitochondrial Dysfunction Through the AMPK/Sirt1/NF-κB Signaling Pathway Article Snippet: Samples were permeabilized with 0.1% Triton X-100 for 10 minutes prior to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining ( C10617 , Invitrogen) at 37 °C for 1 hour. .. After Microscopy:Article Title: Ginkgetin Alleviates Doxorubicin-Induced Heart Failure by Regulating Mitochondrial Dysfunction Through the AMPK/Sirt1/NF-κB Signaling Pathway Article Snippet: Samples were permeabilized with 0.1% Triton X-100 for 10 minutes prior to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining ( C10617 , Invitrogen) at 37 °C for 1 hour. .. After |
