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FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow <t>cytometry</t> (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.
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FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow <t>cytometry</t> (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.
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FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow <t>cytometry</t> (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.
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FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow <t>cytometry</t> (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.
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FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow cytometry (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.

Journal: ACS Catalysis

Article Title: Ultrahigh-Throughput Activity Engineering of Promiscuous Amidases through a Fluorescence-Activated Cell Sorting Assay

doi: 10.1021/acscatal.5c01903

Figure Lengend Snippet: FACS-based detection of amidase activity. ( A ) Overview of the FACS-based assay principle. Fluorescence histograms of E. coli BL21­(DE3)-Gold cells expressing GST only as a negative control ( B ) or co-expressing GST and the amidase Pd Amd ( C ) or Sa Amd ( D ), respectively. Amidase activity in whole cells towards 1 was measured by flow cytometry (FL3-UV: λ ex = 375 nm/λ em = 440 nm); 1 and 1-GSH do not exhibit fluorescence.

Article Snippet: Flow cytometry analysis was conducted with a Partec CyFlow space device (Sysmex Partec GmbH, Görlitz, Germany).

Techniques: Activity Assay, Fluorescence, Expressing, Negative Control, Flow Cytometry

Comparison of flow cytometry variables among groups

Journal: Journal of Clinical Laboratory Analysis

Article Title: Association of lymphocyte subsets with mortality in severe COVID‐19 pneumonia patients

doi: 10.1002/jcla.24046

Figure Lengend Snippet: Comparison of flow cytometry variables among groups

Article Snippet: Multicolor flow cytometry device (PARTEK CYFLOW SPACE, Germany) and EXBIO antibody kits (Spain) were used for lymphocytes immunophenotyping.

Techniques: Comparison, Flow Cytometry, Cell Counting

Assessments of relationships between flow cytometry variables with ICU admission and mortality

Journal: Journal of Clinical Laboratory Analysis

Article Title: Association of lymphocyte subsets with mortality in severe COVID‐19 pneumonia patients

doi: 10.1002/jcla.24046

Figure Lengend Snippet: Assessments of relationships between flow cytometry variables with ICU admission and mortality

Article Snippet: Multicolor flow cytometry device (PARTEK CYFLOW SPACE, Germany) and EXBIO antibody kits (Spain) were used for lymphocytes immunophenotyping.

Techniques: Flow Cytometry