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cycloleucine cl  (Tokyo Chemical Industry)
90
Tokyo Chemical Industry cycloleucine cl
Betaine treatment enhanced OGD/R treated primary neuronal death and CL treatment attenuated OGD/R-treated primary neuronal death. (A–C) Primary neurons were treated with 8 mM Betaine or 20mM CL and then cultured under OGD/R conditions or normoxia. Cell death was quantified by CCK8 assay or by microscopy for PI-positive cells. Quantifications of the percentage of dead cells were shown. (mean ± SEM; n = 6–20; * p < 0.05, *** p < 0.001 versus OGD/R). (D–E) Representative images of Western blots of primary neuron treated with 8 mM Betaine or 20 mM CL. Cleaved-caspase3 levels were determined by Western blot (mean ± SEM; n = 3; * p < 0.05 versus OGD/R). (F) A proposed model for Alkbh5 and Fto in cerebral ischemia-reperfusion injury. CCK8, Cell Counting Kit-8; CL, <t>cycloleucine;</t> OGD/R, glucose oxygen deprivation/reoxygenation; PI, propidium iodide; SEM, standard error of the mean.
Cycloleucine Cl, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycloleucine+cl/cycloleucine/pmc07222229-98-11-14
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cycloleucine cl - by Bioz Stars, 2026-10
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1-amino-cyclopentane-l[´cl]carboxylic acid (cycloleucine)  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc 1-amino-cyclopentane-l[´cl]carboxylic acid (cycloleucine)
Betaine treatment enhanced OGD/R treated primary neuronal death and CL treatment attenuated OGD/R-treated primary neuronal death. (A–C) Primary neurons were treated with 8 mM Betaine or 20mM CL and then cultured under OGD/R conditions or normoxia. Cell death was quantified by CCK8 assay or by microscopy for PI-positive cells. Quantifications of the percentage of dead cells were shown. (mean ± SEM; n = 6–20; * p < 0.05, *** p < 0.001 versus OGD/R). (D–E) Representative images of Western blots of primary neuron treated with 8 mM Betaine or 20 mM CL. Cleaved-caspase3 levels were determined by Western blot (mean ± SEM; n = 3; * p < 0.05 versus OGD/R). (F) A proposed model for Alkbh5 and Fto in cerebral ischemia-reperfusion injury. CCK8, Cell Counting Kit-8; CL, <t>cycloleucine;</t> OGD/R, glucose oxygen deprivation/reoxygenation; PI, propidium iodide; SEM, standard error of the mean.
1 Amino Cyclopentane L[´Cl]Carboxylic Acid (Cycloleucine), supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycloleucine+cl/1+amino+cyclopentane+l+%C2%B4cl+carboxylic+acid++cycloleucine+/10__1016_slash_s0021___9258_ascii40_17_ascii41_33286___6-28-0-8
Average 90 stars, based on 1 article reviews
1-amino-cyclopentane-l[´cl]carboxylic acid (cycloleucine) - by Bioz Stars, 2026-10
90/100 stars
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Betaine treatment enhanced OGD/R treated primary neuronal death and CL treatment attenuated OGD/R-treated primary neuronal death. (A–C) Primary neurons were treated with 8 mM Betaine or 20mM CL and then cultured under OGD/R conditions or normoxia. Cell death was quantified by CCK8 assay or by microscopy for PI-positive cells. Quantifications of the percentage of dead cells were shown. (mean ± SEM; n = 6–20; * p < 0.05, *** p < 0.001 versus OGD/R). (D–E) Representative images of Western blots of primary neuron treated with 8 mM Betaine or 20 mM CL. Cleaved-caspase3 levels were determined by Western blot (mean ± SEM; n = 3; * p < 0.05 versus OGD/R). (F) A proposed model for Alkbh5 and Fto in cerebral ischemia-reperfusion injury. CCK8, Cell Counting Kit-8; CL, cycloleucine; OGD/R, glucose oxygen deprivation/reoxygenation; PI, propidium iodide; SEM, standard error of the mean.

Journal: Therapeutic Advances in Chronic Disease

Article Title: N 6 -methyladenosine demethylases Alkbh5/Fto regulate cerebral ischemia-reperfusion injury

doi: 10.1177/2040622320916024

Figure Lengend Snippet: Betaine treatment enhanced OGD/R treated primary neuronal death and CL treatment attenuated OGD/R-treated primary neuronal death. (A–C) Primary neurons were treated with 8 mM Betaine or 20mM CL and then cultured under OGD/R conditions or normoxia. Cell death was quantified by CCK8 assay or by microscopy for PI-positive cells. Quantifications of the percentage of dead cells were shown. (mean ± SEM; n = 6–20; * p < 0.05, *** p < 0.001 versus OGD/R). (D–E) Representative images of Western blots of primary neuron treated with 8 mM Betaine or 20 mM CL. Cleaved-caspase3 levels were determined by Western blot (mean ± SEM; n = 3; * p < 0.05 versus OGD/R). (F) A proposed model for Alkbh5 and Fto in cerebral ischemia-reperfusion injury. CCK8, Cell Counting Kit-8; CL, cycloleucine; OGD/R, glucose oxygen deprivation/reoxygenation; PI, propidium iodide; SEM, standard error of the mean.

Article Snippet: Neurons were cultured in vitro for neuron standard medium supplemented with cycloleucine (CL) [A1063, TCI (Shanghai) Chemical Industry Development Co., Ltd.] (10 mM, 20 mM, 40 mM) and betaine (61962, Sigma) (4 mM, 8 mM, 16 mM) from 24 h before OGD to the end of the experiment.

Techniques: Cell Culture, CCK-8 Assay, Microscopy, Western Blot, Cell Counting