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Omega Bio Tek mircoelute cyclepure kit
Mircoelute Cyclepure Kit, supplied by Omega Bio Tek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cyclepure+kit/microelute+cycle+pure+kit/pmc11536340-193-0-3
Average 90 stars, based on 1 article reviews
mircoelute cyclepure kit - by Bioz Stars, 2026-09
90/100 stars

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Amplification:

Article Title: Probiotic Escherichia coli inhibits biofilm formation of pathogenic E. coli via extracellular activity of DegP
Article Snippet: .. The degP gene was amplified by PCR with degPfd and degPrv primers (Supplementary Table ) using Pfu polymerase at 98 °C for 30 s, with 35 cycles of 98 °C for 30 s, 54 °C for 30 s, and 72 °C for 1 min, as well as a final extension of 72 °C for 5 min followed by DNA purification using a CyclePure kit (Omega Bio-Tek, Norcross, GA). .. The pCA24N plasmid and degP PCR products were digested with restriction enzymes BseRI and HindIII (New England Biolabs, Ipswich, MA, USA) and purified.

Polymerase Chain Reaction:

Article Title: Probiotic Escherichia coli inhibits biofilm formation of pathogenic E. coli via extracellular activity of DegP
Article Snippet: .. The degP gene was amplified by PCR with degPfd and degPrv primers (Supplementary Table ) using Pfu polymerase at 98 °C for 30 s, with 35 cycles of 98 °C for 30 s, 54 °C for 30 s, and 72 °C for 1 min, as well as a final extension of 72 °C for 5 min followed by DNA purification using a CyclePure kit (Omega Bio-Tek, Norcross, GA). .. The pCA24N plasmid and degP PCR products were digested with restriction enzymes BseRI and HindIII (New England Biolabs, Ipswich, MA, USA) and purified.

Article Title: Differential effects of two earthworm species on Fusarium wilt of strawberry
Article Snippet: Earthworms can reduce soil-borne diseases via diverse mechanisms.. The differential effects of different earthworm species on soil-borne diseases, and the mechanisms that account for the differences, however, have been largely ignored.. To assess the effects of different earthworm species on Fusarium wilt in replanted strawberry and the underlying mechanisms involves, a greenhouse experiment in which plants were placed in soil with earthworms (either Metaphire guillemi (M) or Eisenia fetida (E)) or without earthworms (CK) was carried out.

Article Title: Construction and analysis of a SSH cDNA library of Eucalyptus grandis × Eucalyptus urophylla 9224 induced by Cylindrocladium quinqueseptatum
Article Snippet: Leaf blight caused by Cylindrocladium quinqueseptatum Boedijn & Reitsma is a major disease occurring in Eucalyptus plantlets and saplings worldwide.. Current studies on the resistance to this disease in eucalypti have focused primarily on analyzing the relationship between the disease and the metabolic products from the anatomic structure of Eucalyptus leaves and their physiological responses to pathogen invasion.. There have been no reports on the mechanisms of molecular responses.

DNA Purification:

Article Title: Probiotic Escherichia coli inhibits biofilm formation of pathogenic E. coli via extracellular activity of DegP
Article Snippet: .. The degP gene was amplified by PCR with degPfd and degPrv primers (Supplementary Table ) using Pfu polymerase at 98 °C for 30 s, with 35 cycles of 98 °C for 30 s, 54 °C for 30 s, and 72 °C for 1 min, as well as a final extension of 72 °C for 5 min followed by DNA purification using a CyclePure kit (Omega Bio-Tek, Norcross, GA). .. The pCA24N plasmid and degP PCR products were digested with restriction enzymes BseRI and HindIII (New England Biolabs, Ipswich, MA, USA) and purified.

Purification:

Article Title: Differential effects of two earthworm species on Fusarium wilt of strawberry
Article Snippet: Earthworms can reduce soil-borne diseases via diverse mechanisms.. The differential effects of different earthworm species on soil-borne diseases, and the mechanisms that account for the differences, however, have been largely ignored.. To assess the effects of different earthworm species on Fusarium wilt in replanted strawberry and the underlying mechanisms involves, a greenhouse experiment in which plants were placed in soil with earthworms (either Metaphire guillemi (M) or Eisenia fetida (E)) or without earthworms (CK) was carried out.

DNA Extraction:

Article Title: Establishment and Maintenance of Gnotobiotic American Cockroaches ( Periplaneta americana )
Article Snippet: Cycle Pure kit; D6493 may also be used; includes buffers (purifying, wash, elution) Hybridization oven UVP 95–0330–01 we use a hybridization oven for preheating elution buffer, but a water bath could probably also be used Laminar flow biological safety cabinet NuAire, Inc. NU-425–400 Protocol refers to this as “laminar flow hood” for brevity peracetic acid stock solution (32%) Sigma-Aldrich 269336 Petroleum jelly Vaseline n/a RsaI New England BioLabs R0167 Includes CutSmart (digestion) buffer Secondary container n/a n/a a plastic container with a lid (such as a Kritter Keeper) works well for this (25cm long x 15cm wide x 22cm high); it should be large enough to fit BHI slants and test tubes spectrophotometer ThermoFisher ND-2000 Catalog info is for NanoDrop2000 thermal shaker Eppendorf EP5386000028 Thermomixer R Tris-EDTA Fisher BP1338–1 10 nm Tris, 1 mM EDTA, pH 8 Woodchip bedding P.J. .. Murphy Forest Products Sani-Chips proteinase K buffer Omega Bio-Tek PD061 included in Omega Biotek’s bacterial DNA extraction kit (“TL buffer”) binding buffer Omega Bio-Tek PD099 included in Omega Biotek’s bacterial DNA extraction kit (“HBC” buffer) wash buffer Omega Bio-Tek PDR044 included in Omega Biotek’s bacterial DNA extraction kit (“DNA wash” buffer) elution buffer Omega Bio-Tek PDR048 included in Omega Biotek’s bacterial DNA extraction kit purifying buffer Omega Bio-Tek PDR042 included in Omega Biotek’s CyclePure kit (“CP” buffer) lysozyme Omega Bio-Tek n/a included in Omega Biotek’s bacterial DNA extraction kit glass beads Omega Bio-Tek n/a included in Omega Biotek’s bacterial DNA extraction kit Open in a separate window 6. ..

Binding Assay:

Article Title: Establishment and Maintenance of Gnotobiotic American Cockroaches ( Periplaneta americana )
Article Snippet: Cycle Pure kit; D6493 may also be used; includes buffers (purifying, wash, elution) Hybridization oven UVP 95–0330–01 we use a hybridization oven for preheating elution buffer, but a water bath could probably also be used Laminar flow biological safety cabinet NuAire, Inc. NU-425–400 Protocol refers to this as “laminar flow hood” for brevity peracetic acid stock solution (32%) Sigma-Aldrich 269336 Petroleum jelly Vaseline n/a RsaI New England BioLabs R0167 Includes CutSmart (digestion) buffer Secondary container n/a n/a a plastic container with a lid (such as a Kritter Keeper) works well for this (25cm long x 15cm wide x 22cm high); it should be large enough to fit BHI slants and test tubes spectrophotometer ThermoFisher ND-2000 Catalog info is for NanoDrop2000 thermal shaker Eppendorf EP5386000028 Thermomixer R Tris-EDTA Fisher BP1338–1 10 nm Tris, 1 mM EDTA, pH 8 Woodchip bedding P.J. .. Murphy Forest Products Sani-Chips proteinase K buffer Omega Bio-Tek PD061 included in Omega Biotek’s bacterial DNA extraction kit (“TL buffer”) binding buffer Omega Bio-Tek PD099 included in Omega Biotek’s bacterial DNA extraction kit (“HBC” buffer) wash buffer Omega Bio-Tek PDR044 included in Omega Biotek’s bacterial DNA extraction kit (“DNA wash” buffer) elution buffer Omega Bio-Tek PDR048 included in Omega Biotek’s bacterial DNA extraction kit purifying buffer Omega Bio-Tek PDR042 included in Omega Biotek’s CyclePure kit (“CP” buffer) lysozyme Omega Bio-Tek n/a included in Omega Biotek’s bacterial DNA extraction kit glass beads Omega Bio-Tek n/a included in Omega Biotek’s bacterial DNA extraction kit Open in a separate window 6. ..

Sequencing:

Article Title: Comparison of seven hepatitis B virus (HBV) nucleic acid testing assays in selected samples with discrepant HBV marker results from United States blood donors.
Article Snippet: BACKGROUND: Sensitive triplex nucleic acid tests (NATs) are implemented for blood donation screening worldwide.. Assays have variable ability to detect lowlevel hepatitis B virus (HBV) DNA.. At borderline DNA detection levels, where Poisson distribution impacts results, distinguishing true-positive from false-positive results is challenging.



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6. Quality control of sterility

Journal: Journal of visualized experiments : JoVE

Article Title: Establishment and Maintenance of Gnotobiotic American Cockroaches ( Periplaneta americana )

doi: 10.3791/61316

Figure Lengend Snippet: 6. Quality control of sterility

Article Snippet: Murphy Forest Products Sani-Chips proteinase K buffer Omega Bio-Tek PD061 included in Omega Biotek’s bacterial DNA extraction kit (“TL buffer”) binding buffer Omega Bio-Tek PD099 included in Omega Biotek’s bacterial DNA extraction kit (“HBC” buffer) wash buffer Omega Bio-Tek PDR044 included in Omega Biotek’s bacterial DNA extraction kit (“DNA wash” buffer) elution buffer Omega Bio-Tek PDR048 included in Omega Biotek’s bacterial DNA extraction kit purifying buffer Omega Bio-Tek PDR042 included in Omega Biotek’s CyclePure kit (“CP” buffer) lysozyme Omega Bio-Tek n/a included in Omega Biotek’s bacterial DNA extraction kit glass beads Omega Bio-Tek n/a included in Omega Biotek’s bacterial DNA extraction kit Open in a separate window 6.

Techniques: Modification, DNA Extraction, Binding Assay, DNA Purification, Hybridization, Spectrophotometry