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The number of informative reads (log 2 ) identified by the mNGS assay used to establish the presence or absence of a target is inversely proportional to <t>PCR</t> <t>cycle</t> thresholds (C t value). Each point represents a DHM sample with colour and shape indicating true positive (TP; blue square), false positive (FP; red triange), and false negative (FN; pink circle) results when comparing mNGS assay and PCR test results. The best fit line considers only TP samples and only targets with >5 TP samples are shown. The red horizontal line denotes a C t value of 35.
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The number of informative reads (log 2 ) identified by the mNGS assay used to establish the presence or absence of a target is inversely proportional to PCR cycle thresholds (C t value). Each point represents a DHM sample with colour and shape indicating true positive (TP; blue square), false positive (FP; red triange), and false negative (FN; pink circle) results when comparing mNGS assay and PCR test results. The best fit line considers only TP samples and only targets with >5 TP samples are shown. The red horizontal line denotes a C t value of 35.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Metagenomics enables parallel detection of 176 clinically relevant targets from faecal samples

doi: 10.3389/fcimb.2026.1759322

Figure Lengend Snippet: The number of informative reads (log 2 ) identified by the mNGS assay used to establish the presence or absence of a target is inversely proportional to PCR cycle thresholds (C t value). Each point represents a DHM sample with colour and shape indicating true positive (TP; blue square), false positive (FP; red triange), and false negative (FN; pink circle) results when comparing mNGS assay and PCR test results. The best fit line considers only TP samples and only targets with >5 TP samples are shown. The red horizontal line denotes a C t value of 35.

Article Snippet: We explored the relationship between the Seegene PCR cycle threshold (C t ) values obtained by Microba Laboratories and the number of informative sequencing reads identified by the mNGS assay ( ).

Techniques: